Antibacterial stilbenes from the tubers of Bletilla striata

Jiang, Sai; Chen, Changfen; Ma, Xiao‐Pan; Wang, Mengyun; Wang, Wei; Yu, Xia; Ni, Zhaohui; Ming-kai, WU; Pan, Weidong

doi:10.1016/j.fitote.2019.104350

Cited by 28 publications

(12 citation statements)

References 30 publications

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“…In traditional medicine, B. striata has been widely used for thousands of years to stop bleeding mainly from the stomach and lungs and for detumescence. Modern pharmacology research has further proven that B. striata fights against bacteria (Li et al 2014;Guo et al 2016;Jiang et al 2019), influenza A virus (Shi et al 2017), fibrosis (Wang et al 2014) and tumorigenesis (Zhan et al 2014) and promotes wound (Diao et al 2008;Luo et al 2010) and oral ulcer healing (Liao et al 2019). Additionally, B. striata is used as a hemostatic agent since it promotes rapid blood coagulation (Hung and Wu 2016;Zhang et al 2017).…”

Section: Introductionmentioning

confidence: 99%

Selection of Reference Genes for qRT-PCR in Bletilla striata under Heat Stress

Liang¹

2021

IJAB

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Bletilla striata (Thunb.) Reihb.f., a traditional Chinese herbal medicine, has attracted increasing attention because of its wide range of applicability to the medical field and chemical industry. B. striata has been identified to be particularly sensitive to high temperatures. Thus, the study of temperature stress on gene transcription is of interest in the field. Use of reliable reference genes is essential for qRT-PCR analysis of genes. However, little information regarding suitable reference genes for the genus Bletilla has been published. In this study, the sequences of seven potential reference genes in B. striata were obtained via a homology cloning strategy. These genes were as follows: glyceraldehydes-3-phosphate dehydrogenase (GAPDH), 18S ribosomal RNA (18S), elongation factor 1 alpha (EF1α), α-tubulin (TUA), β-tubulin (TUB), ubiquitin (UBI), and NAC domain protein (NAC). We then evaluated the stability levels of these transcripts in different tissues (root, tuber, and leaf) exposed to high temperature using three conventional software and comprehensive RefFinder algorithms. The results indicated that 18S and TUB were the best internal control genes among different periods of heat treatment and that a combination of 18S and UBI was the best in different tissues. Altogether, 18S and UBI were identified to be the best reference genes for all the samples, while NAC and TUA were the least stable reference genes. The results will be useful for studies on target gene expression in plants of the Bletilla genus. © 2021 Friends Science Publishers© 2021 Friends Science Publishers© 2021 Friends Science Publishers© 2021 Friends Science Publishers© 2021 Friends Science Publishers© 2021 Friends Science Publishers© 2021 Friends Science Publishers© 2021 Friends Science Publishers© 2021 Friends Science Publishers© 2021 Friends Science Publishers© 2021 Friends Science Publishers© 2021 Friends Science Publishers© 2021 Friends Science Publishers© 2021 Friends Science Publishers© 2021 Friends Science Publishers© 2021 Friends Science Publishers© 2021 Friends Science Publishers© 2021 Friends Science Publishers© 2021 Friends Science Publishers© 2021 Friends Science Publishers© 2021 Friends Science Publishers© 2021 Friends Science Publishers© 2021 Friends Science Publishers© 2021 Friends Science Publishers © 2021 Friends Science Publishers© 2021 Friends Science Publishers© 2021 Friends Science Publishers© 2021 Friends Science Publishers© 2021 Friends Science Publishers© 2021 Friends Science Publishers© 2021 Friends Science Publishers©

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Section: Introductionmentioning

confidence: 99%

Selection of Reference Genes for qRT-PCR in Bletilla striata under Heat Stress

Liang¹

2021

IJAB

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“…Rchb.f. (Orchidaceae) [74], a plant used by traditional Chinese medicine to treat hematemesis, tuberculosis, malignant ulcers, traumatic bleeding, and cold [75], 21 compounds were isolated and evaluated for their antimicrobial activities against MRSA S. aureus ATCC 43300, B. subtilis ATCC 6051, S. aureus ATCC 6538, and E. coli ATCC 11775 (Figure 9, Table 13). Compounds 18a, 19a, 19c, 20, 21, and 23 were shown to be active against S. aureus ATCC 6538 (MIC values ranging from 6 to 52 µ g/mL).…”

Section: Combretastatins and Their Analoguesmentioning

confidence: 99%

“…Compounds 18a, 19a, 19c, 20, 21, and 23 were shown to be active against S. aureus ATCC 6538 (MIC values ranging from 6 to 52 µ g/mL). The methoxy groups seemed to decrease the antibacterial activity (compound 19a vs 19c), and the phenanthrenes were shown to be better than the corresponding dihydrophenanthrenes against MRSA S. aureus (compound 18a versus 19a) [74]. Sciryagarol I and II (Figure 9) are two cis-stilbenoids isolated from the tubers of Scirpus yagara Ohwi (perennial Cyperaceae species), which is used in traditional Chinese medicine.…”

Section: Combretastatins and Their Analoguesmentioning

confidence: 99%

Stilbenoids: A Natural Arsenal against Bacterial Pathogens

et al. 2020

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The escalating emergence of resistant bacterial strains is one of the most important threats to human health. With the increasing incidence of multi-drugs infections, there is an urgent need to restock our antibiotic arsenal. Natural products are an invaluable source of inspiration in drug design and development. One of the most widely distributed groups of natural products in the plant kingdom is represented by stilbenoids. Stilbenoids are synthesised by plants as means of protection against pathogens, whereby the potential antimicrobial activity of this class of natural compounds has attracted great interest in the last years. The purpose of this review is to provide an overview of recent achievements in the study of stilbenoids as antimicrobial agents, with particular emphasis on the sources, chemical structures, and the mechanism of action of the most promising natural compounds. Attention has been paid to the main structure modifications on the stilbenoid core that have expanded the antimicrobial activity with respect to the parent natural compounds, opening the possibility of their further development. The collected results highlight the therapeutic versatility of natural and synthetic resveratrol derivatives and provide a prospective insight into their potential development as antimicrobial agents.

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“…Bletilla striata, a perennial medicinal herb of the Orchidaceae family, is primarily distributed across Hubei, Sichuan, Yunnan, Shaanxi, Guizhou, Jiangxi, and Jiangsu Provinces of China (Chen et al 2019, Jiang et al 2019. As a traditional Chinese medicine, it has been used in curing alimentary canal mucosal damage, ulcers, bruises, and burns for thousands of years (Xu et al 2019.…”

Section: Introductionmentioning

confidence: 99%

Establishment of a Rapid Breeding System for Bletilla Striata

Liu

et al. 2020

Preprint

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BackgroundBletilla striata (Thunb. ex A. Murray) Rchb. f., a species of perennial herb of orchidaceae has remarkable effects and high economic value; thus it has been intensively studied by many scholars. Although this herb has numerous seeds, the germination rate is exceptionally low, which leads to decreased of germplasm resources and increased market demands year by year. ResultsWith the aim of addressing this issue, the present study included the aseptic germination system and the direct seeding technology system. On the basis of Murashige and Skoog (MS) medium, 2.0 mg/L 6-benzylaminopurine (6-BA) and 1.0 mg/L naphthylacetic acid (NAA) were added prior to seed germination, 70 g/L banana juice and 0.5 mg/L NAA were added when rooting, after which the seedlings were transplanted into a mixed substrate of humus, river sand and bark (3:1:1). The direct seeding system consists of a seedling base and substrate treatment, sowing and seedling raising, seedling growth and transplanting, etc. Turfy soil, huangjiang residue and river sand were selected as the substrate. The results revealed that the germination rate was increased to 91.8%, while the plantlet regeneration was increased to 82.0%. Following 180 days of cultivation, the plants could be transplanted as finished seedlings.ConclusionsThe establishment of this B. striata seedling system provides a safe, rapid, reliable production technology route for its industrial development.

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Antibacterial stilbenes from the tubers of Bletilla striata

Cited by 28 publications

References 30 publications

Selection of Reference Genes for qRT-PCR in Bletilla striata under Heat Stress

Selection of Reference Genes for qRT-PCR in Bletilla striata under Heat Stress

Stilbenoids: A Natural Arsenal against Bacterial Pathogens

Establishment of a Rapid Breeding System for Bletilla Striata

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