Antibacterial Synergistic Effect of Extracts of the Organs of Capparis Spinosa and in Combination With Antibiotics.

Fatima-Zahra, Ennacerie; Filali, FouziaRhazi; Moukrad, Najia; Dra., AbdelazizEd

doi:10.21474/ijar01/5445

Cited by 8 publications

(9 citation statements)

References 16 publications

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“…This observation is in accordance with the findings of Fatma and coworkers, where they discovered that a synergy occurred with Oxacillin antibiotics, a synergistic potential also observed with Ampicillin, Chlorteracycline, Trimethoprim, Augmentine, and Doxicycline. 24 Furthermore, our results agreed with the discovery of Ennacerie et al, 25 who published that the association between extracts and antibiotics showed an increased level of antibacterial potency compared with lone antibiotics. The antibiotics susceptibility test results revealed that S. aureus was the isolate most affected by the test antibiotics, while E. coli showed the highest changes among the test microorganisms.…”

Section: Morgani)supporting

confidence: 88%

Inhibitory Effects of Radish Peeled Root Extracts against Some Pathogenic Bacteria Suha M. Abed*, Lina Q. Yasin, Waqas S. Mahmood

2021

TJNPR

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cell lysis.⁶ The present study was aimed at investigating the antibacterial activities of R. sativus extracts against pathogenic bacteria and detecting differences in virulence factor expression before and after exposure to the crude extracts. Materials and Methods Bacteria and cultural conditionsThe pathogenic bacteria were collected from different clinical cases including urinary tract infection, pharyngitis, otitis media, wounds, and lower respiratory tract infection using cotton swabs or universal sterile cups depending on the specimens. These samples were immediately transported to the laboratory under the supervision of physicians. Bacteria were isolated from the clinical samples and identified using microscopic, and cultural characterization. Biochemical tests under aerobic conditions were performed which included Indole test to investigate the production of indole; methyl red test to investigate sugar fermentation with acid production; Vogus-Proscauer test for acetone compound detection; utilization of citrate as a sole source of carbon and formation of sodium carbonate and urease test to indicate the hydrolysis of urea and formation of ammonium. In addition, an oxidase test was used to investigate the production of cytochrome; catalase and fermentation tests for sugar fermentation; motility screening, and coagulase enzyme tests were carried out in two ways (slide and tube methods) as described by Mahmoodn, 2019. 7 Confirmation of the identity of bacterial isolatesVitek compact technique using automated Vitek device (BioMérieux, Marcy l'Etoile France) was used to confirm the identity of bacterial isolates. Each isolate was cultured on nutrient agar and incubated overnight at 37°C. Bacteria were suspended in 2.5 mL of a 0.45% sodium chloride solution. The suspension used was adjusted to a McFarland standard of 0.5 using a Densicheck 8 . The cards were automatically filled by a vacuum device, sealed, and inserted into the

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Section: Morgani)supporting

confidence: 88%

Inhibitory Effects of Radish Peeled Root Extracts against Some Pathogenic Bacteria Suha M. Abed*, Lina Q. Yasin, Waqas S. Mahmood

2021

TJNPR

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“…For each strain two antibiotics to which they are resistant are tested. The test is carried out on a solid medium according to the protocol described by Ennacerie and his collaborators [9,14,15]. Regarding the negative test, it is presented by a disk impregnated with distilled water or DMSO according to the type of aqueous or ethanolic extract.…”

Section: Synergistic Antibacterial Interactions Between Extracts and Antibiotics: Disk Diffusion Methodsmentioning

confidence: 99%

“…MIC is the minimum concentration of the extract that inhibits the growth of 90% of the bacterial population after incubation for 18 to 24 hours at 37 °C. The determination of the MICs of the plant extracts with respect to the bacterial strains is carried out according to the microtitration technique on microplates described by Ennacerie and his collaborators [7,8,9]. After incubation, the bacterial viability indicator, [2, 3, 5] -triphenyl-2H-tetrazolium chloride (TTC), is added to each well.…”

Section:  Determination Of the Minimum Inhibitory Concentration (Mic)mentioning

confidence: 99%

Design, Development and Characterizations of Acyclovir Osmotic Tablets

Fouzia¹

2018

JPPR

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Study contextual: Faced with the global problem of antimicrobial resistance, and the use of traditional medicine for the research of antibacterial biomolecules. Aim: our work focused on the valorization of a medicinal plant Dittrichia viscosa which has many therapeutic and culinary virtues worldwide. Methods: To do this, a phytochemical screening of the leafy stems of the plant is carried out according to a set of physicochemical reactions, as well as an in vitro evaluation of the antibacterial activity, of the aqueous and ethanolic extracts against multi-resistant bacterial strains, by microdilution technique on microplates. An evaluation of the synergistic interaction between extracts and weakened antibiotics against pathogenic bacteria was also highlighted in this study. Results: The tests revealed the richness of Dittrichia viscosa species by tannins, flavonoids, saponosides, sterols and triterpenes. As for the antibacterial effect, the MICs range from 0.858±0.29 to 66.66 ± 0.00 mg / ml and the MBCs from 4.300 ± 1.01 to 11.610 ± 2.31 mg / ml is an interesting antibacterial activity. Regarding the combination of extracts with antibiotics tested, it revealed a synergistic action inducing an amplification of the antibacterial power of Penicillin, Imipenem and Erythromycin with a rate that reaches 471%. Conclusion: The results of this study show that the aqueous and ethanolic extracts of Dittrichia viscosa have interesting and promising antibacterial activity in the pharmaceutical, food and cosmetic industries.

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“…The dry matter is then milled and kept away from moisture until the extracts are prepared. Preparation methods of the extracts of the plant Two types of extracts, decoctat at 10% and ethanolic, were prepared from the powder of flower buds and fruits of Capparis spinosa according to the method described by Ennacerie et al [13] Dosage of total phenolic content Total phenolic content of extracts was determined; it was based on the method of folin ciocalteu method. The acid gallic was used as a standard.…”

Section: Plant Materialsmentioning

confidence: 99%

“…[6] Capparis spinosa is used also in pharmaceutical industry and modern medicine. [1][2][3][4][5][6][7] This plant has also attracted the attention of several researchers, for its many therapeutic virtues such as antimicrobial effect [13] , antiinflammatory [4] and antioxidant activity [14] antiallergic, anti-histamine, and antiviral roles. [11] These criteria allow Capparis spinosa to rank among the plants medicinal and aromatic the most appreciated.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the Antioxidant Activity and the Cytotoxicity of Extracts of Capparis Spinosa

Ennacerie

Filali

Moukrad

et al. 2018

Int. J. Pharm. Sci. Drug Res.

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The purpose of this study is to promote the plant spontaneous, harvested from the West of Morocco and streamline its use in quantifying the total phenolic content, assessing the antioxidant activity and testing the degree of toxicity on two types of eukaryotic cells plants and animals. The content, extracts aqueous and ethanolic flower buds and fruit, in phenolic compounds was determined according to the method Follin- Ciocalteu. The antioxidant activity was evaluated by two methods FRAP and DPPH. As for the cytotoxicity extracts, it has been evaluated by the test hemolysis and inhibition germination test of Lepidium sativum seeds. The different extracts of the two organs of this plant, have revealed richness in total polyphenols, especially those of the flower buds, as well as, an antioxidant activity, which is in the same order as that of vitamin C for the aqueous extract of flower buds. The effect hemolytic is shown positive for decoctat of flower buds. The ethanolic extract of fruits displayed an activity antimitotic expressed by the inhibition of elongation and growth seedlings of Lepedium sativum. The decoctat of flower exhibited an effect antigerminatif of moderate intensity which is reversible after rehydration of seed. Capparis Spinosa is a plant of quality pharmaceutical interesting for its activities antioxidant, antimitotic, healing, and for its wealth in phenolics compounds

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Antibacterial Synergistic Effect of Extracts of the Organs of Capparis Spinosa and in Combination With Antibiotics.

Cited by 8 publications

References 16 publications

Inhibitory Effects of Radish Peeled Root Extracts against Some Pathogenic Bacteria Suha M. Abed*, Lina Q. Yasin, Waqas S. Mahmood

Inhibitory Effects of Radish Peeled Root Extracts against Some Pathogenic Bacteria Suha M. Abed*, Lina Q. Yasin, Waqas S. Mahmood

Design, Development and Characterizations of Acyclovir Osmotic Tablets

Evaluation of the Antioxidant Activity and the Cytotoxicity of Extracts of Capparis Spinosa

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