Antibiofilm Activity of Epoxy/Ag-TiO2 Polymer Nanocomposite Coatings against Staphylococcus Aureus and Escherichia Coli

Abstract

Dispersion of functional inorganic nano-fillers like TiO2 within polymer matrix is known to impart excellent photobactericidal activity to the composite. Epoxy resin systems with Ag + ion doped TiO2 can have combination of excellent biocidal characteristics of silver and the photocatalytic properties of TiO2. The inorganic antimicrobial incorporation into an epoxy polymeric matrix was achieved by sonicating laboratory-made nano-scale anatase TiO2 and Ag-TiO2 into the industrial grade epoxy resin. The resulting epoxy composite had ratios of 0.5-2.0 wt% of nano-filler content. The process of dispersion of Ag-TiO2 in the epoxy resin resulted in concomitant in situ synthesis of silver nanoparticles due to photoreduction of Ag + ion. The composite materials were characterized by DSC and SEM. The glass transition temperature (Tg) increased with the incorporation of the nanofillers over the neat polymer. The materials synthesized were coated on glass petri dish. Anti-biofilm property of coated material due to combined release of biocide, and photocatalytic activity under static conditions in petri dish was evaluated against Staphylococcus aureus ATCC6538 and Escherichia coli K-12 under UV irradiation using a crystal violet binding assay. Prepared composite showed significant inhibition of biofilm development in both the organisms. Our studies indicate that the effective dispersion and optimal release of biocidal agents was responsible for anti-biofilm activity of the surface. The reported thermoset coating materials can be used as bactericidal surfaces either in industrial or healthcare settings to reduce the microbial loads. OPEN ACCESSCoatings 2015, 5 96

Antibacterial, Antibiofilm, Antiquorum Sensing, Antimotility, and Antioxidant Activities of Green Fabricated Ag, Cu, TiO2, ZnO, and Fe3O4 NPs via Protoparmeliopsis muralis Lichen Aqueous Extract against Multi-Drug-Resistant Bacteria

“…It is worth mentioning that the lower effect of Fe 3 O 4 NPs on biofilm formation may be a result of the chelation mechanisms of iron by biofilm structure. , Antibiofilm activity of copper NPs was reported as higher values of 90.58% for P. aeruginosa PACI02 clinical pathogens . Nanocomposite by 1.5 wt % of Ag-TiO 2 had complete reduction in biofilm of S. aureus and E. coli during 24 h treatment . ZnO NPs demonstrated an antibiofilm property on E. coli O157:H7, methicillin-resistance S. aureus (MRSA), methicillin-sensitive S. aureus (MSSA), and P. aureuginosa PAO1 strains at 1, 5, 5, and 1 mM concentrations .…”

Recent progress on Ag/TiO2 photocatalysts: photocatalytic and bactericidal behaviors

“…Despite all merits previously presented, photocatalytic water treatment, assisted by nanosized nanoparticles, shows some drawbacks that limit its practical applications. In fact, the difficulty of separation and recovery of Ag/TiO 2 nanoparticles from aqueous solutions during the photocatalytic process, in addition to the difficulty to apply on continuous flow systems pushed many researchers to immobilize silver-doped TiO 2 nanoparticles on different materials such as carbonaceous nanomaterials (Chen et al 2014;Lee et al 2015;Noreen et al 2019), magnetic materials (Tedsree et al 2017;Mehrvar 2018;Scott et al 2019), polymers, and biopolymers materials (Singh et al 2014;Rtimi et al 2015;Santhosh and Natarajan 2015;Jbeli et al 2018;Li et al 2018b;Haghighat et al 2019). This section aims to present the different supports used to overcome the problems related to the recovery of the Ag/TiO 2 nanoparticles after the photocatalysis process.…”

Investigating the effects of zinc oxide and titanium dioxide nanoparticles on the formation of biofilm and persister cells in Klebsiella pneumoniae

“…The study by Curtis et al on L. monocytogenes also concluded that MazEF is also activated under antibiotic stress and thus exhibits long‐term resilience [27]. In 2013, the study by Santosh et al demonstrated the anti‐biofilm activity of epoxy/Ag‐TiO 2 polymers against E. coli and Staphylococcus strains, but the exact mechanism was not defined [28].…”