Antibiofilm and Antiadhesive Activities of Different Synbiotics

Stefania, De Marco; Mir, Piccioni; Diana, Muradyan; Zadra, Claudia; Pagiotti, Rita; Pietrella, Donatella

doi:10.4172/2329-8901.1000182

Cited by 16 publications

(15 citation statements)

References 34 publications

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“…Other authors proved that metabolites from S. boulardii had a bacteriostatic effect on cells of S. aureus in amount of 0.1 mL and showed no activity against E. coli (Stefania et al, 2017). Individual sensitivity of test cultures to substances of saccharomycetes, observed by Stefania et al (2017), has been confirmed by our results. The polyresistant strain K. pneumoniae PR demonstrated higher sensitivity to biologically active substances of L. rhamnosus GG compared to disintegrates and metabolites from S. boulardii.…”

Section: Discussionsupporting

confidence: 82%

Evaluation of anti-microbial activity of filtrates of Lactobacillus rhamnosus and Saccharomyces boulardii against antibiotic-resistant gram-negative bacteria

Isayenko

Knysh

Kotsar

et al. 2019

Regul. Mech. Biosyst.

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The article presents the results of the first study on the influence of biologically active substances Lactobacillus rhamnosus GG ATCC 53103 and Saccharomyces boulardii, obtained according to the author`s method, on growth of gram-negative bacteria with broad medical resistance: Pseudomonas aeruginosa PR, Klebsiella pneumoniae PR, Lelliottia amnigena (Enterobacter amnigenus) PR using the spectrophotometric method. Disintegrates of L. rhamnosus GG and S. boulardii were obtained using low-frequency ultrasound processing of suspension of probiotic strains, and metabolites – through cultivation of lactobacteria and saccharomycetes in disintegrates of probiotic microorganisms. To samples of test-cultures with studied filtrates of disintegrates or metabolites we added growth medium and cultivated them (period of monitoring was 5- and 24-hours). Results of the studies were expressed as the percentage of inhibition of increment in polyresistant gram-negative bacteria under the impact of biologically active substances of probiotic microorganisms. Five-hour incubation of test-strains with the studied samples of lactobacteria led to inhibition of their growth properties by 85.6–96.7%. Growth of bacteria under the impact of substances of saccharomycetes was inhibted by 45.1–92.5%. Twenty-four hour exposure of the test-cultures in filtrates of L. rhamnosus GG and S. boulardii caused 100% inhibition of P. aeruginosa and L. amnigena polyresistant strains. Temporal interval of cultivation directly proportionally affected the extent of inhibition of growth of microorganisms: we determined direct correlation dependence within 0.789–0.991. Maximum inhibition of increment of the studied pathogens was observed under the influence of metabolites of lactobacteria, obtained by cultivating primary producers in their disintegrate. We determined a high level of anti-microbial activity of metabolites from L. rhamnosus GG and S. boulardii obtained by cultivation of probiotics in disintegrates against bacteria resistant to a broad range of preparations, which allows us to consider these substances as promising for development of anti-microbial preparations of a new generation against etiologically significant antibiotic-resistant gram-negative microorganisms.

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Section: Discussionsupporting

confidence: 82%

Evaluation of anti-microbial activity of filtrates of Lactobacillus rhamnosus and Saccharomyces boulardii against antibiotic-resistant gram-negative bacteria

Isayenko

Knysh

Kotsar

et al. 2019

Regul. Mech. Biosyst.

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“…As shown in Fig. 2, cell-free extract B in the composition of the cultivation medium at a concentration of 10 % does not have a significant effect on the optical density gain of the culture compared to PC 10 . An increase in the content of this extract in the composition of the cultivation medium to 30 % and 50 % leads to a significant increase of the culture optical density gain compared to the PC 30 and PC 50 (StIs are 19.5 % and 16.3 %, respectively).…”

Section: Resultsmentioning

confidence: 86%

“…One way to increase the survival of bacteria is to protect them by encapsulation or adhesion to a semi-permeable biocompatible dextranomer microsphere in the form of a biofilm [8]. The second way is to use growth promoting prebiotic ingredients: β-galacto-oligosaccharides, lactulose, fructo-oligosaccharides, inulin, isomaltose [10]. An alternative approach to increasing colonization of the gastrointestinal tract by beneficial microorganisms and enhancing the protective properties of the immune system is using the biological activity of the probiotics' derivatives [11][12][13].…”

mentioning

confidence: 99%

The effects of cell-free extracts derived from probiotic strains Bifidobacterium bifidum and Lactobacillus reuteri on the proliferation and biofilm formation by Lactobacillus reuteri in vitro

Knysh

2019

ZMJ

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The aim of the research was to investigate the ability of cell-free extracts, containing derivatives of probiotic strains Bifidobacterium bifidum and Lactobacillus reuteri, to influence the proliferation and biofilm formation by Lactobacillus reuteri in vitro; to evaluate the prospects for the creation of new metabiotics and means of increasing the overall productivity of probiotic cell biomass based on them.Materials and methods. Cell-free extracts were obtained from probiotic strains B. bifidum 1 and L. reuteri DSM 17938 by the authors' method. Probiotic strain L. reuteri DSM 17938 used as a test culture. The investigation of the proliferation and biofilm formation by L. reuteri was carried out by spectrophotometric method using a microtiter-plate reader "Lisa Scan EM" (Erba Lachema s.r.o., Czech Republic). Results.It has been established that the cell-free extract obtained from L. reuteri culture grown in its own disintegrate, supplemented with glycerol and glucose, is the only one of all studied, which stimulates both proliferation and biofilm formation by L. reuteri. The cell-free extracts, obtained from L. reuteri and B. bifidum disintegrates and from L. reuteri culture, grown in its own disintegrate, stimulate proliferation of test-culture to varying degrees (depending on the type of extract and its content in the culture medium), but have a significant inhibitory effect on the biofilm formation by L. reuteri. The extract, obtained from B. bifidum culture, grown in its own disintegrate, does not have a significant effect on proliferation and greatly suppresses the biofilm formation by L. reuteri. Conclusions.The results of the study allow us to positively evaluate the prospects for the creation of new metabiotics based on probiotic derivatives. Cell-free extract, obtained from L. reuteri culture, grown in its own disintegrate supplemented with glycerol and glucose can contribute to the survival and facilitate inoculation of the introduced probiotic in the gastrointestinal tract when used together.Derivative-containing extracts with a pronounced growth-stimulating effect can be the basis for creating the means of increasing the overall productivity of the probiotic cell biomass. The use of such means will increase the economic efficiency of probiotic cultures production. Obtained data induce further study of the biochemical composition, elucidation of the mechanism of cellfree extracts action and confirmation of their efficacy in vivo. Вплив безклітинних екстрактів, що отримані з пробіотичних штамів Bifidobacterium bifidum та Lаctobacillus reuteri, на проліферацію та біоплівкоутворення Lactobacillus reuteri in vitro О. В. Книш Мета роботи -дослідити здатність безклітинних екстрактів, що містять деривати пробіотичних штамів Bifidobacterium bifidum і Lactobacillus reuteri, впливати на проліферацію та біоплівкоутворення Lactobacillus reuteri in vitro; оцінити перспективи створення на їхній основі нових метабіотиків, засобів підвищення загальної продуктивності клітинної біомаси пробіотика. Матеріали та методи. ...

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“…Mamianas et al stated that L. plantarum has good antimicrobial effects against S. aureus, E. coli and Bacillus subtilis [33]. To date, only a few carbohydrates have been defined and reported as prebiotics, including inulin, lactulose, β-galacto-oligosaccharides, and fructooligosaccharides [29]. In the present study, raffinose, trehalose, riboflavin, citrate, inulin, and sorbitol was used as prebiotic.…”

Section: Discussionmentioning

confidence: 94%

“…Antibacterial activity (MIC and MBC) of cell-free supernatants of probiotics growth in absence or in presence of prebiotics against clinical isolates of MDR A. baumannii were shown in Table 3. The MIC was recorded as µl of bacterial culture supernatants at a concentration of 10 8 bacteria/ml [29]. All probiotics exhibited an antibacterial effect against MDR A. baumannii.…”

Section: Micro-broth Dilution Methodsmentioning

confidence: 99%

Antibacterial and Antibiofilm Effects of Synbiotics against Multidrug-Resistant bacteria: Acinetobacter baumannii and Enterococcus faecalis

Laal-Kargar

Dolatabadi

Mohtashami

2020

Preprint

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Background: Acinetobacter baumannii and Enterocoocus faecalis increase their resistance against antibiotic by producing biofilm. Antibiotic resistance has become a massive public health threat that require novel effective antibacterial and antibiofilm alternatives. The use of probiotics is interested to prevent and control certain infections. The objective of this study was to investigate the antibacterial and antibiofilm property of probiotics and synbiotics against multidrug-resistant A. baumannii and E. faecalis. Methods: The antimicrobial and the antibiofilm activities of cell- free supernatants of four strains of Lactobacillus against 20 clinical multi-drug resistant (MDR) isolates of Acinetobacter baumannii and Enterocoocus faecalis were determined in the presence of 0.3% of sorbitol, raffinose, citrate, trehalose, inulin, and riboflavin using well diffusion agar and micro-dilution method. Results: The cell- free supernatant of L. rhamnosus with citrate and trehalose showed the best antibacterial activity against MDR A. baumannii (28.8±2.1mm, 1.128 μL/mL), and L. rhamnosus with all of prebiotics against MDR E. faecalis (29.8±0 mm, 1.128 μL/mL) compare to probiotic alone. The prebiotics could improve the inhibitory effect of probiotics against the Gram-negative A. baumannii higher than Gram-positive E. faecalis. Biofilm formation was reduced in both pathogens in presence of synbiotics. L. plantarum with riboflavin and L. rhamnosus with or without inulin potently inhibits E. faecalis (50±0.86%) and A. baumannii (75±6.5%) biofilm formation, respectively. Conclusions: The results of current study support the antibiofilm activity of metabolites produced by synbiotics, and suggest their use as suitable adjuvants as well as biocontrol agents for treatment.

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Antibiofilm and Antiadhesive Activities of Different Synbiotics

Cited by 16 publications

References 34 publications

Evaluation of anti-microbial activity of filtrates of Lactobacillus rhamnosus and Saccharomyces boulardii against antibiotic-resistant gram-negative bacteria

Evaluation of anti-microbial activity of filtrates of Lactobacillus rhamnosus and Saccharomyces boulardii against antibiotic-resistant gram-negative bacteria

The effects of cell-free extracts derived from probiotic strains Bifidobacterium bifidum and Lactobacillus reuteri on the proliferation and biofilm formation by Lactobacillus reuteri in vitro

Antibacterial and Antibiofilm Effects of Synbiotics against Multidrug-Resistant bacteria: Acinetobacter baumannii and Enterococcus faecalis

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