Antibiofilm and antimicrobial activity of <scp><i>Lactobacillus</i></scp> cell free supernatant against <scp><i>Pseudomonas aeruginosa</i></scp> isolated from burn wounds

Asadzadegan, Reza; Haratian, Negar; Sadeghi, Mahsa; Maroufizadeh, Saman; Mobayen, Mohammadreza; Sedigh Ebrahim Saraei, Hadi; Hasannejad‐Bibalan, Meysam

doi:10.1111/iwj.14305

Cited by 5 publications

(1 citation statement)

References 73 publications

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“…aimed to study the influence of cell‐free supernatants (CFS) obtained from the culture of probiotic Lactobacillus strains on the pathogenicity of P. aeruginosa . [ 221 ] When the agar/skim milk culture medium was inoculated with CFS, there was no clearance zone around the P. aeruginosa colony after 1 day of culture, in contrast to the control group that received no CFS. These findings suggest that CFS mitigated the pathogenicity of P. aeruginosa by either inhibiting the activity of proteases secreted by P. aeruginosa or, as the authors of that paper believe, inhibiting the protease production by P. aeruginosa .…”

Section: Controlling the Protease Expression And Activitymentioning

confidence: 99%

Approaches to Control and Monitor Protease Levels in Chronic Wounds

Kolahreez,

Ghasemi‐Mobarakeh,

Liebner

et al. 2024

Advanced Therapeutics

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Some wounds do not follow the typical healing trajectory and show very little, if any, progress. They dreadfully threaten the patients' quality of life and may even lead to limb amputation and death. Several interrelated systemic and local factors may cause a delay in wound healing. The microenvironment of these wounds could be different from wounds that heal spontaneously or with standard treatment. As discussed in the present work, proteases’ levels or activity could be elevated in some cases of chronic ulcers; infection could aggravate the situation. A detailed overview of the proteases’ effect on wound healing is presented in this paper. Assuming untimely or irregularly excessive proteolytic activity plays a pathological role, some researchers targeted attenuation of protease level as a strategy to promote healing. This could be done via different approaches, including physical removal, inhibiting their activity, and controlling their expression directly through gene silencing or indirectly, for instance, by resolving infection. Apart from targeting protease modulation as a therapeutic strategy, many studies have examined the protease state to gain insights into the underlying mechanisms by which a remedy, dressing, or specific therapy has affected the healing process. This article aims to provide an overview of the studies in these two categories. Of course, various proteases are involved in the healing process, but in each study, usually, only one or some of them were investigated; matrix metalloproteinase‐2 (MMP‐2), MMP‐9, and human neutrophil elastase (HNE) are among them. Furthermore, in some cases, cumulative proteolytic activity has been evaluated in terms of gelatinolytic, collagenolytic, or caseinolytic activity, which is also absolutely beneficial. Some research groups have considered the potential of proteases as a diagnostic or prognostic biomarker and have tried to develop some sensors or indicators; this topic has also been covered in the present work.This article is protected by copyright. All rights reserved

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Section: Controlling the Protease Expression And Activitymentioning

confidence: 99%

Approaches to Control and Monitor Protease Levels in Chronic Wounds

Kolahreez,

Ghasemi‐Mobarakeh,

Liebner

et al. 2024

Advanced Therapeutics

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Antibacterial mechanism of Lactiplantibacillus plantarum SHY96 cell-free supernatant against Listeria monocytogenes revealed by metabolomics and potential application on chicken breast meat preservation

Liang,

Wang,

Zhou

et al. 2025

Food Chemistry: X

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Dipeptides from Lactiplantibacillus plantarum limit Pseudomonas aeruginosa pathogenesis

Narasimulu,

Baburajan,

Saravanan

et al. 2024

Journal of Applied Microbiology

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Aims Lactobacilli have drawn a great deal of attention due to the heterogeneity of their cells, as well as their remarkable ability to adapt to a range of harsh conditions. One such adaptation response by Lactiplantibacillus plantarum was forming a V-shaped cell structure in low acidic pH conditions. This study aims to identify low pH induced-biofilm formation in L. plantarum and Limosilactobacillus reuteri and their antagonistic interactions with an opportunistic pathogen, Pseudomonas aeruginosa PAO1. Methods and Results Dual species bacterial interactions revealed a Lactobacilli biofilm-mediated suppression of PAO1 biofilms and pyocyanin pigment. Furthermore, filtrates from Lactobacilli grown at low acidic pH (pH 3.5) effectively arrest the pathogenesis of P. aeruginosa PAO1 including biofilm, swarming motility, and pyocyanin formation. Liquid chromatography-mass spectroscopy analysis revealed the presence of several Lactobacilli secreted metabolites and dipeptides that exhibited strong LasR receptor antagonistic interactions in molecular docking analysis. Prolyl-tyrosine showed the best lasR antagonistic and PAO1 biofilm inhibitory activities of the identified dipeptides. Conclusion Overall, the results will contribute to the development of anti-infective drugs against P. aeruginosa using Lactobacilli secreted dipeptides. Furthermore, the investigation identifies several dipeptides that can be extracted from V-shaped Lactobacilli for antimicrobial protein therapy against P. aeruginosa and other pathogens.

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Antibiofilm and antimicrobial activity of Lactobacillus cell free supernatant against Pseudomonas aeruginosa isolated from burn wounds

Cited by 5 publications

References 73 publications

Approaches to Control and Monitor Protease Levels in Chronic Wounds

Approaches to Control and Monitor Protease Levels in Chronic Wounds

Antibacterial mechanism of Lactiplantibacillus plantarum SHY96 cell-free supernatant against Listeria monocytogenes revealed by metabolomics and potential application on chicken breast meat preservation

Dipeptides from Lactiplantibacillus plantarum limit Pseudomonas aeruginosa pathogenesis

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