Antibiofilm and Antiquorum Sensing Potential of Lactiplantibacillus plantarum Z057 against Vibrio parahaemolyticus

Han, Xiangpeng; Chen, Qingying; Zhang, Xingguo; Chen, Xiaolan; Luo, Dongsheng; Qin, Zhong

doi:10.3390/foods11152230

Cited by 10 publications

(4 citation statements)

References 56 publications

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“…The formylchromone 6B3FC suppressed the expression of opaR , luxS , vmrA , and tdh ( Figure 5 ). In the bacterium, V. parahaemolyticus , related to quorum sensing genes ( opaR and luxS) are crucial regulators of bacterial biofilm formation ( Han et al., 2022 ). Two key regulatory elements, opaR and aphA , are responsible for maintaining the integrity of the luxS QS system.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial and antibiofilm activities of formylchromones against Vibrio parahaemolyticus and Vibrio harveyi

Sathiyamoorthi,

Lee,

Tan

et al. 2023

Front. Cell. Infect. Microbiol.

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Gram-negative Vibrio species are major foodborne pathogens often associated with seafood intake that causes gastroenteritis. On food surfaces, biofilm formation by Vibrio species enhances the resistance of bacteria to disinfectants and antimicrobial agents. Hence, an efficient antibacterial and antibiofilm approach is urgently required. This study examined the antibacterial and antivirulence effects of chromones and their 26 derivatives against V. parahaemolyticus and V. harveyi. 6-Bromo-3-formylchromone (6B3FC) and 6-chloro-3-formylchromone (6C3FC) were active antibacterial and antibiofilm compounds. Both 6B3FC and 6C3FC exhibited minimum inhibitory concentrations (MICs) of 20 µg/mL for planktonic cell growth and dose-dependently inhibited biofilm formation. Additionally, they decreased swimming motility, protease activity, fimbrial agglutination, hydrophobicity, and indole production at 20 µg/mL which impaired the growth of the bacteria. Furthermore, the active compounds could completely inhibit the slimy substances and microbial cells on the surface of the squid and shrimp. The most active compound 6B3FC inhibited the gene expression associated in quorum sensing and biofilm formation (luxS, opaR), pathogenicity (tdh), and membrane integrity (vmrA) in V. parahaemolyticus. However, toxicity profiling using seed germination and Caenorhabditis elegans models suggests that 6C3FC may have moderate effect at 50 µg/mL while 6B3FC was toxic to the nematodes 20-100 µg/mL. These findings suggest chromone analogs, particularly two halogenated formylchromones (6B3FC and 6C3FC), were effective antimicrobial and antibiofilm agents against V. parahaemolyticus in the food and pharmaceutical sectors.

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Section: Discussionmentioning

confidence: 99%

Antimicrobial and antibiofilm activities of formylchromones against Vibrio parahaemolyticus and Vibrio harveyi

Sathiyamoorthi,

Lee,

Tan

et al. 2023

Front. Cell. Infect. Microbiol.

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“…According to Han et al, the anti-QS abilities of L. plantarum Z057 against V. parahaemolyticus were found to be remarkable. It was observed that Z057-E could effectively impede EPS production as well as inhibit the QS signaling molecule AI-2 and extracellular protein secretions [68]. Many studies have been dedicated to interactions of lactobacilli with QS signals from Gram-positive bacteria besides than their ability to inhibit QS signaling in Gram-negative bacteria via interactions with various AHL types.…”

Section: Anti-virulence Treatment Strategies 41 Secondary Metabolites...mentioning

confidence: 99%

Section: Anti-virulence Treatment Strategiesmentioning

confidence: 99%

New Strategies for Biocontrol of Bacterial Toxins and Virulence: Focusing on Quorum-Sensing Interference and Biofilm Inhibition

Zhang,

et al. 2023

Toxins

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The overuse of antibiotics and the emergence of multiple-antibiotic-resistant pathogens are becoming a serious threat to health security and the economy. Reducing antimicrobial resistance requires replacing antibiotic consumption with more biocontrol strategies to improve the immunity of animals and humans. Probiotics and medicinal plants have been used as alternative treatments or preventative therapies for a variety of diseases caused by bacterial infections. Therefore, we reviewed some of the anti-virulence and bacterial toxin-inhibiting strategies that are currently being developed; this review covers strategies focused on quenching pathogen quorum sensing (QS) systems, the disruption of biofilm formation and bacterial toxin neutralization. It highlights the probable mechanism of action for probiotics and medicinal plants. Although further research is needed before a definitive statement can be made on the efficacy of any of these interventions, the current literature offers new hope and a new tool in the arsenal in the fight against bacterial virulence factors and bacterial toxins.

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“…Deleting the 01,912 genes of P. marina increased the colanic acid and exopolysaccharide levels, thus promoting the settlement of mussels [ 6 ]. OmpW, a prominent OMP of EPS [ 29 , 37 ], has been proven to regulate biofilm formation in other bacteria [ 28 , 38 ]. The interaction between OmpW and biofilm and how the outer membrane regulator impact OmpW remains unclear.…”

Section: Introductionmentioning

confidence: 99%

Two-Component System Response Regulator ompR Regulates Mussel Settlement through Exopolysaccharides

Wang

Zhang

et al. 2023

IJMS

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The outer membrane protein (OMP) is a kind of biofilm matrix component that widely exists in Gram-negative bacteria. However, the mechanism of OMP involved in the settlement of molluscs is still unclear. In this study, the mussel Mytilus coruscus was selected as a model to explore the function of ompR, a two-component system response regulator, on Pseudoalteromonas marina biofilm-forming capacity and the mussel settlement. The motility of the ΔompR strain was increased, the biofilm-forming capacity was decreased, and the inducing activity of the ΔompR biofilms in plantigrades decreased significantly (p < 0.05). The extracellular α-polysaccharide and β-polysaccharide of the ΔompR strain decreased by 57.27% and 62.63%, respectively. The inactivation of the ompR gene decreased the ompW gene expression and had no impact on envZ expression or c-di-GMP levels. Adding recombinant OmpW protein caused the recovery of biofilm-inducing activities, accompanied by the upregulation of exopolysaccharides. The findings deepen the understanding of the regulatory mechanism of bacterial two-component systems and the settlement of benthic animals.

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Antibiofilm and Antiquorum Sensing Potential of Lactiplantibacillus plantarum Z057 against Vibrio parahaemolyticus

Cited by 10 publications

References 56 publications

Antimicrobial and antibiofilm activities of formylchromones against Vibrio parahaemolyticus and Vibrio harveyi

Antimicrobial and antibiofilm activities of formylchromones against Vibrio parahaemolyticus and Vibrio harveyi

New Strategies for Biocontrol of Bacterial Toxins and Virulence: Focusing on Quorum-Sensing Interference and Biofilm Inhibition

Two-Component System Response Regulator ompR Regulates Mussel Settlement through Exopolysaccharides

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