Antibiofilm and antivirulence properties of chitosan-polypyrrole nanocomposites to Pseudomonas aeruginosa

Khan, Fazlurrahman; Manivasagan, Panchanathan; Pham, Dung Thuy Nguyen; Oh, Junghwan; Kim, Shin‐Kwon; Kim, Young‐Mog

doi:10.1016/j.micpath.2019.01.033

Cited by 56 publications

(33 citation statements)

References 72 publications

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“…The crystal violet method was used for the quantitative estimation of biofilm formation in the presence and absence of the compound, following the procedure described earlier [8]. The minimum concentration of F-AuNP that inhibited P. aeruginosa biofilm formation (minimum biofilm inhibition concentration: MBIC) was also determined.…”

Section: Methodsmentioning

confidence: 99%

“…The minimum concentration at which F-AuNPs exhibited eradication effect on pre-formed matured biofilm (minimum biofilm eradication concentration: MBEC) was also determined. The first step was to allow the formation of biofilm for 24 h by incubating P. aeruginosa in TSB without F-AuNPs in the 96 well microtiter plate, as discussed earlier [8]. Briefly, after incubation, the planktonic cells were removed and attached biofilm cells were washed thrice with fresh TSB media.…”

Section: Methodsmentioning

confidence: 99%

“…Visualization of the cell morphology and biofilm architecture was carried out by using microscopes such as the scanning electron microscopy (SEM) and fluorescence microscopy. The procedure used for the SEM sample preparation was adopted as discussed earlier [8,32]. Briefly, the cell culture was allowed to grow in TSB media on the surface of nylon membranes (0.5 × 0.5 cm) placed in a 24 well microtiter plate in the presence and absence of F-AuNPs (256 µg/mL).…”

Section: Methodsmentioning

confidence: 99%

“…With the rapid pace of emergence and spread of P. aeruginosa with biofilm-forming ability, current anti-biofilm and anti-virulence approaches have mainly targeted the following: (1) attachment of planktonic cells, (2) cell-to-cell communication networks and regulatory systems, and (3) eradication of pre-existing matured biofilm structures [6,7]. Furthermore, these modern anti-biofilm approaches highly favor treatments which are bioactive, cost-effective, and less toxic [8,9,10,11].…”

Section: Introductionmentioning

confidence: 99%

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Fucoidan-Stabilized Gold Nanoparticle-Mediated Biofilm Inhibition, Attenuation of Virulence and Motility Properties in Pseudomonas aeruginosa PAO1

et al. 2019

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The emergence of antibiotic resistance in Pseudomonas aeruginosa due to biofilm formation has transformed this opportunistic pathogen into a life-threatening one. Biosynthesized nanoparticles are increasingly being recognized as an effective anti-biofilm strategy to counter P. aeruginosa biofilms. In the present study, gold nanoparticles (AuNPs) were biologically synthesized and stabilized using fucoidan, which is an active compound sourced from brown seaweed. Biosynthesized fucoidan-stabilized AuNPs (F-AuNPs) were subjected to characterization using UV-visible spectroscopy, Fourier transform infrared spectroscopy (FTIR), field emission transmission electron microscopy (FE-TEM), dynamic light scattering (DLS), and energy dispersive X-ray diffraction (EDX). The biosynthesized F-AuNPs were then evaluated for their inhibitory effects on P. aeruginosa bacterial growth, biofilm formation, virulence factor production, and bacterial motility. Overall, the activities of F-AuNPs towards P. aeruginosa were varied depending on their concentration. At minimum inhibitory concentration (MIC) (512 µg/mL) and at concentrations above MIC, F-AuNPs exerted antibacterial activity. In contrast, the sub-inhibitory concentration (sub-MIC) levels of F-AuNPs inhibited biofilm formation without affecting bacterial growth, and eradicated matured biofilm. The minimum biofilm inhibition concentration (MBIC) and minimum biofilm eradication concentration (MBEC) were identified as 128 µg/mL. Furthermore, sub-MICs of F-AuNPs also attenuated the production of several important virulence factors and impaired bacterial swarming, swimming, and twitching motilities. Findings from the present study provide important insights into the potential of F-AuNPs as an effective new drug for controlling P. aeruginosa-biofilm-related infections.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Fucoidan-Stabilized Gold Nanoparticle-Mediated Biofilm Inhibition, Attenuation of Virulence and Motility Properties in Pseudomonas aeruginosa PAO1

et al. 2019

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“…The impacts of UA-CS NPs on the cell morphology of bacteria was checked under scanning electron microscopy (SEM) as described earlier with slight modification [84]. Briefly, the overnight grown cells were diluted (1:100) in their respective growth media and treated with sub-MIC of UA-CS NPs.…”

Section: Analysis Of Cell Morphology Of Bacterial Cells Treated By Uamentioning

confidence: 99%

Bactericidal Activity of Usnic Acid-Chitosan Nanoparticles against Persister Cells of Biofilm-Forming Pathogenic Bacteria

Khan

Kim

2020

Marine Drugs

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The present study aimed to prepare usnic acid (UA)-loaded chitosan (CS) nanoparticles (UA-CS NPs) and evaluate its antibacterial activity against biofilm-forming pathogenic bacteria. UA-CS NPs were prepared through simple ionic gelification of UA with CS, and further characterized using Fourier transform infrared spectroscopy, X-ray diffraction, and field-emission transmission electron microscopy. The UA-CS NPs presented a loading capacity (LC) of 5.2%, encapsulation efficiency (EE) of 24%, and a spherical shape and rough surface. The maximum release of UA was higher in pH 1.2 buffer solution as compared to that in pH 6.8 and 7.4 buffer solution. The average size and zeta potential of the UA-CS NPs was 311.5 ± 49.9 nm in diameter and +27.3 ± 0.8 mV, respectively. The newly prepared UA-CS NPs exhibited antibacterial activity against persister cells obtained from the stationary phase in batch culture, mature biofilms, and antibiotic-induced gram-positive and gram-negative pathogenic bacteria. Exposure of sub-inhibitory concentrations of UA-CS NPs to the bacterial cells resulted in a change in morphology. The present study suggests an alternative method for the application of UA into nanoparticles. Furthermore, the anti-persister activity of UA-CS NPs may be another possible strategy for the treatment of infections caused by biofilm-forming pathogenic bacteria.

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Fabrication of Conductive Polypyrrole Doped Chitosan Thin Film for Sensitive Detection of Sulfite in Real Food and Biological Samples

2020

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In this study, we reported electrochemical synthesis of conductive polypyrrole‐chitosan (PPY‐CHI) thin film for sensitive detection of sulfite in real samples. The synthesized PPY‐CHI film was characterized in terms of surface morphology, optical property, binding energy, conductivity and electrochemical properties. The synthesized copolymeric PPY‐CHI film displayed good electrocatalytic behaviour towards oxidation of sulfite. The synthesized PPY‐CHI film was used for sulfite detection using differential pulse voltammetric technique with detection limit, sensitivity and linearity of 0.21 μM (S/N=3), 15.28 μA μM cm−2 and 50–1100 μM respectively. In addition, the current responses of PPY‐CHI film towards sulfite were repeatable, reproducible response and unaffected by selected electroactive interferents. Finally, the synthesized PPY‐CHI was successfully and satisfactorily applied for determination of sulfite in real food and biological samples. The results obtained from this study highly placed PPY‐CHI film as a promising sensor for sensitive and accurate detection of sulfite in food and biological samples for human health protection.

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Antibiofilm and antivirulence properties of chitosan-polypyrrole nanocomposites to Pseudomonas aeruginosa

Cited by 56 publications

References 72 publications

Fucoidan-Stabilized Gold Nanoparticle-Mediated Biofilm Inhibition, Attenuation of Virulence and Motility Properties in Pseudomonas aeruginosa PAO1

Fucoidan-Stabilized Gold Nanoparticle-Mediated Biofilm Inhibition, Attenuation of Virulence and Motility Properties in Pseudomonas aeruginosa PAO1

Bactericidal Activity of Usnic Acid-Chitosan Nanoparticles against Persister Cells of Biofilm-Forming Pathogenic Bacteria

Fabrication of Conductive Polypyrrole Doped Chitosan Thin Film for Sensitive Detection of Sulfite in Real Food and Biological Samples

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