Antibiofilm potential of flavonoids extracted fromMoringa oleiferaseed coat againstStaphylococcus aureus,Pseudomonas aeruginosaandCandida albicans

Onsare, Jemimah Gesare; Arora, Daljit Singh

doi:10.1111/jam.12701

Cited by 106 publications

(58 citation statements)

References 34 publications

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“…Due to the presence of major compounds in the leaves of S. obtusifolium and S. cumini that are known to have antifungal activity, such as total flavonoids (Onsare & Arora 2014;Yixi et al 2015) and saponins (Sparg et al 2004;Coleman et al 2010), respectively, and in view of the potential sustainability of their use Note that the column 'polyphenols' refers to a general measurement of all polyphenolic compounds in the sample, including, for instance, flavonoids. In this case, the same compound might have been quantified in both 'total flavonoids' and 'polyphenols' analyses.…”

Section: Discussionmentioning

confidence: 99%

“…Likely, the predominance of flavonoids in the Nb fraction may have favored its antiCandida properties. Flavonoids extracted from different plant species were found to have strong inhibitory effects on the formation and metabolic activity of C. albicans biofilms or planktonic cells (Onsare & Arora 2014), which means that this phytochemical class could be a marker for antifungal activity.…”

Section: Discussionmentioning

confidence: 99%

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Antifungal potential ofSideroxylon obtusifoliumandSyzygium cuminiand their mode of action againstCandida albicans

Pereira

Freires

Castilho

et al. 2016

Pharmaceutical Biology

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Context The emergence of resistant pathogens and toxicity of antifungals have encouraged an active search for novel candidates to manage Candida biofilms. Objective In this study, the little known species Sideroxylon obtusifolium T.D. Penn (Sapotacea) and Syzygium cumini (L.) Skeels (Myrtaceae), from the Caatinga biome in Brazil were chemically characterized and explored for their antifungal potential against C. albicans. Materials and methods We determined the effects of hydroalcoholic extracts/fractions upon fungal growth (minimum inhibitory and fungicidal concentrations, MIC/MFC), biofilm morphology (scanning electron microscopy) and viability (confocal laser scanning microscopy), proposed their mode of action (sorbitol and ergosterol assays), and finally investigated their effects against macrophage and keratinocyte cells in a cell-based assay. Data were analysed using one-way analysis of variance with Tukey-Kramer post-test (a ¼ 0.05). Results The n-butanol (Nb) fraction from S. obtusifolium and S. cumini extract (Sc) showed flavonoids (39.11 6 6.62 mg/g) and saponins (820.35 6 225.38 mg/g), respectively, in their chemical composition and demonstrated antifungal activity, with MICs of 62.5 and 125 lg/mL, respectively. Nb and Sc may complex with ergosterol as there was a 4-16-fold increase in MICs in the presence of exogenous ergosterol, leading to disrupted permeability of cell membrane. Deleterious effects were observed on morphology and viability of treated biofilms from concentrations as low as their MICs and higher. Sc was not toxic to macrophages and keratinocytes at these concentrations (p > 0.05), unlike Nb. Conclusions Nb and Sc demonstrated considerable antifungal activity and should be further investigated as potential alternative candidates to treat Candida biofilms. ARTICLE HISTORY

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Antifungal potential ofSideroxylon obtusifoliumandSyzygium cuminiand their mode of action againstCandida albicans

Pereira

Freires

Castilho

et al. 2016

Pharmaceutical Biology

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“…Catechins of tea displayed strong reduction of viable cells during biofilm formation of Candida albicans (Evensen and Braun, 2009). A fraction rich in flavonoids extracted from Moringa oleifera seed coat also showed antibiofilm activity against this yeast (Onsare and Arora, 2014).…”

Section: Antibiofilm Activitymentioning

confidence: 95%

Phytochemical investigation and antimicrobial assessment of Bellis sylvestris leaves

Scognamiglio

Buommino

Coretti

et al. 2016

Phytochemistry Letters

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“…Stock solutions were prepared and the minimum inhibitory concentration was determined using a range of concentrations, i.e., from 0.05 to 2% (equivalent to 0.5–20 mg ml −1 ) for aqueous extract, from 0.001 to 0.5% (0.01–5 mg ml −1 ) (organic) and from 0.001 to 1% (0.01–10 mg ml −1 ) (phytoconstituents) against test organisms, as described earlier by Onsare and Arora (2015). The MICs were compared with standard antibiotics (gentamicin and chloramphenicol) and amphotericin B, taken as positive controls.…”

Section: Phytochemical Analysis Of Gymnema Sylvestrementioning

confidence: 59%

In vitro antimicrobial potential of extracts and phytoconstituents from Gymnema sylvestre R.Br. leaves and their biosafety evaluation

Arora

Sood

2017

AMB Expr

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The in vitro antimicrobial screening of Gymnema sylvestre leaves against 13 test pathogens established its broad spectrum activity with average inhibition zone ranging from 14 to 23 mm. The antimicrobial activity of the classically- optimized aqueous extract was enhanced up to 1.45 folds, when subjected to statistical optimization using Response Surface Methodology (RSM) and was thermostable. Ethyl acetate was found to be the best organic extractant with Klebsiella pneumoniae 1 (31.5 mm) and Staphylococcus epidermidis (25.5 mm) being the most sensitive among Gram negative and Gram positive bacteria, respectively. Among the major group of phytoconstituents detected, tannins were the most abundant followed by flavonoids and phytosterols, while triterpenes were absent. Flavonoids and cardiac glycosides exhibited a broad range of antimicrobial potential, with inhibition zone ranging from 13 to 35 mm, where Candida albicans was the most sensitive organism. Ethyl acetate extract showed better potency with lowest Minimum inhibitory concentration (0.1–1 mg ml−1) than the aqueous extract (1–3 mg ml−1) and all partially purified phytoconstituents (0.1–10 mg ml−1). The ethyl acetate extract and flavonoids were highly potent, as they exhibited a total activity potency ranging from 41.4 to 1045 ml g−1. Time kill studies revealed their microbicidal action, where ethyl acetate extract had a kill time from 0 to 12 h. However, among phytoconstituents, flavonoids were the most effective (0–8 h). The MIC and time kill study was also compared to that of standard antibiotics. These findings indicate that Gymnema sylvestre can be a potential source for development of leading metabolites against pathogens of clinical importance like Pseudomonas aeruginosa, Candida albicans, Escherichia coli, Staphylococcus aureus etc. They were neither mutagenic nor cytotoxic, as revealed by Ames and MTT assay.Electronic supplementary materialThe online version of this article (doi:10.1186/s13568-017-0416-z) contains supplementary material, which is available to authorized users.

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Antibiofilm potential of flavonoids extracted fromMoringa oleiferaseed coat againstStaphylococcus aureus,Pseudomonas aeruginosaandCandida albicans

Abstract: Antibiofilm potential and biosafety of plant-based flavonoids from M. oleifera seed coat reveal a prospective active principle that could be of use in biofilm-associated menace.

Cited by 106 publications

References 34 publications

Antifungal potential ofSideroxylon obtusifoliumandSyzygium cuminiand their mode of action againstCandida albicans

Antifungal potential ofSideroxylon obtusifoliumandSyzygium cuminiand their mode of action againstCandida albicans

Phytochemical investigation and antimicrobial assessment of Bellis sylvestris leaves

In vitro antimicrobial potential of extracts and phytoconstituents from Gymnema sylvestre R.Br. leaves and their biosafety evaluation

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