Antibiofilm Properties of Antiseptic Agents Used on Pseudomonas aeruginosa Isolated from Diabetic Foot Ulcers

Barrigah-Benissan, Koko; Ory, Jérôme; Dunyach-Rémy, Catherine; Pouget, Cassandra; Lavigne, Jean‐Philippe; Sotto, Albert

doi:10.3390/ijms231911270

Cited by 11 publications

(8 citation statements)

References 61 publications

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“…The aim of the study was to compare the efficacy of low hypochlorite-containing solutions vs. polyhexanide-containing antiseptic. The reason behind this agenda was the contradictory results concerning the antimicrobial (including antibiofilm) potential of these earlier antiseptics ( Barrigah-Benissan et al., 2022 ; Severing et al., 2022 ). The inclusion of the polyhexanide-base antiseptic, served as the control setting of experiments because antimicrobial/antibiofilm potential of PHMB is generally accepted and recognized in the in vitro and clinical studies) ( To et al., 2016 ; Kramer et al., 2018 ).…”

Section: Discussionmentioning

confidence: 99%

Comparison of antibiofilm activity of low-concentrated hypochlorites vs polyhexanide-containing antiseptic

Paleczny

Junka²,

Krzyżek³

et al. 2023

Front. Cell. Infect. Microbiol.

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Chronic wound infection is highly associated with morbidity and endangers the patient's life. Therefore, wound care products must have a potent antimicrobial and biofilm-eradicating effect. In this work, the antimicrobial/antibiofilm activity of two low-concentrated chlorine-based and releasing solutions was investigated on a total of 78 strains of methicillin-resistant Staphylococcus aureus, Pseudomonas aeruginosa, and Candida albicans, using the cohesive spectrum of in vitro settings, including microtiter plate models, biofilm-oriented antiseptic test, cellulose-based biofilm model, biofilm bioreactors and Bioflux model. The antiseptic containing polyhexamethylene biguanide was used in the character of usability control of performed tests. The results obtained by static biofilm models indicate that low-concentrated chlorine-based and releasing solutions display none to moderate antibiofilm activity, while data obtained by means of the Bioflux model, providing flow conditions, indicate the moderate antibiofilm activity of substances compared with the polyhexanide antiseptic. Considering in vitro data presented in this manuscript, the earlier reported favorable clinical results of low-concentrated hypochlorites should be considered rather an effect of their rinsing activity combined with low cytotoxicity but not the antimicrobial effect per se. For the treatment of heavily biofilm-infected wounds, polyhexanide should be considered the agent of choice because of its higher efficacy against pathogenic biofilms.

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Section: Discussionmentioning

confidence: 99%

Comparison of antibiofilm activity of low-concentrated hypochlorites vs polyhexanide-containing antiseptic

Paleczny

Junka²,

Krzyżek³

et al. 2023

Front. Cell. Infect. Microbiol.

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“…In our practice, we use a solution of sodium hypochlorite/hypochlorous acid <0.08% (NaOCl/HOCl). The NaOCl has been proven in vitro to have efficiency against the biofilm formed by Staphylococcus aureus (SA), Staphylococcus epidermidis (SE), Pseudomonas aeruginosa (PA) and other wound pathogens, which are commonly encountered in DSWI 2,16,17 . As described in 1915 by Henry Drysdale Dakin, the Dakin solution classically contains 0.5% buffered sodium hypochlorite 18 .…”

Section: Discussionmentioning

confidence: 99%

“…The NaOCl has been proven in vitro to have efficiency against the biofilm formed by Staphylococcus aureus (SA), Staphylococcus epidermidis (SE), Pseudomonas aeruginosa (PA) and other wound pathogens, which are commonly encountered in DSWI. 2 , 16 , 17 As described in 1915 by Henry Drysdale Dakin, the Dakin solution classically contains 0.5% buffered sodium hypochlorite. 18 As the active ingredients in this solution can have a toxic effect on different cells like fibroblasts, macrophages, or stratum corneum, lower doses of this antiseptic solution need to be used in open wounds, as the toxicity appears to be dose‐related.…”

Section: Discussionmentioning

confidence: 99%

The role of negative pressure wound therapy with instillation and dwell time in the treatment of deep sternal wound infections—A retrospective cohort study

Bota

Taqatqeh

Bönke

et al. 2023

Health Science Reports

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Background and Aims: Negative pressure wound therapy (NPWT) has gained a central role in the treatment of deep sternal wound infections (DSWIs) after median thoracotomy. Our study aims at proving the safety of using NPWT with instillation and dwell time (NPWTi-d) in the treatment of DSWI.Methods: We retrospectively evaluated the patients who were treated at our institution between March 2018 and November 2021 for DSWI after radical sternectomy using NPWT or NPWTi-d. The NPWTi-d was applied to start the first postoperative day using 75 mmHg negative pressure for 3 h, followed by instillation of sodium hypochlorite <0.08% with a 3-min dwell time. Results:The NPWTi-d group showed a shorter length of stay (29.39 ± 12.09 vs. 39.54 ± 17.07 days; p = 0.049), a shorter elapsed time between the debridement and the flap coverage (7.18 ± 4.27 vs. 11.86 ± 7.7 days; p = 0.003) and less operative or nonoperative dressing changes (1.73 ± 1.14 vs. 2.68 ± 56; p < 0.001). The in-hospital mortality was 8.2%, with no significant differences between the two groups (p = 1). Conclusion:NPWTi-d can be safely employed in the treatment of DSWI. Further prospective randomized studies need to establish the role of NPWTi-d in the control of infection and biofilm as well as in wound healing.

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Section: Experimental Assessment Of Anti-biofilm Activitymentioning

confidence: 99%

“…In vitro studies have demonstrated efficacy of PHMB on a Gram-negative bacteria and Gram-positive bacteria, [43][44][45][46][47] fungi, [48][49][50] certain viruses, 51,52 and single-celled parasites (amoeba). 53,54 PHMB has also been shown to show antibiofilm activity on biofilms, 31,47,48,[55][56][57][58] including wound biofilms. 59,60 Octenidine has also been demonstrated to have antibiofilm activity in in vitro biofilm model assays, 61 and a number of studies have shown HOCl to exhibit antibiofilm activity.…”

Section: Experimental Assessment Of Anti-biofilm Activitymentioning

confidence: 99%

Effectiveness of a polyhexamethylene biguanide-containing wound cleansing solution using experimental biofilm models

Rippon

Rogers

Westgate³

et al. 2023

J Wound Care

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Objective: Antiseptics are widely used in wound management to prevent or treat wound infections, and have been shown to have antibiofilm efficacy. The objective of this study was to assess the effectiveness of a polyhexamethylene biguanide (PHMB)-containing wound cleansing and irrigation solution on model biofilm of pathogens known to cause wound infections compared with a number of other antimicrobial wound cleansing and irrigation solutions. Method: Staphylococcus aureus and Pseudomonas aeruginosa single-species biofilms were cultured using microtitre plate and Centers for Disease Control and Prevention (CDC) biofilm reactor methods. Following a 24-hour incubation period, the biofilms were rinsed to remove planktonic microorganisms and then challenged with wound cleansing and irrigation solutions. Following incubation of the biofilms with a variety of concentrations of the test solutions (50%, 75% or 100%) for 20, 30, 40, 50 or 60 minutes, remaining viable organisms from the treated biofilms were quantified. Results: The six antimicrobial wound cleansing and irrigation solutions used were all effective in eradicating Staphylococcus aureus biofilm bacteria in both test models. However, the results were more variable for the more tolerant Pseudomonas aeruginosa biofilm. Only one of the six solutions (sea salt and oxychlorite/NaOCl-containing solution) was able to eradicate Pseudomonas aeruginosa biofilm using the microtitre plate assay. Of the six solutions, three (a solution containing PHMB and poloxamer 188 surfactant, a solution containing hypochlorous acid (HOCl) and a solution containing NaOCl/HOCl) showed increasing levels of eradication of Pseudomonas aeruginosa biofilm microorganisms with increasing concentration and exposure time. Using the CDC biofilm reactor model, all six cleansing and irrigation solutions, except for the solution containing HOCl, were able to eradicate Pseudomonas aeruginosa biofilms such that no viable microorganisms were recovered. Conclusion: This study demonstrated that a PHMB-containing wound cleansing and irrigation solution was as effective as other antimicrobial wound irrigation solutions for antibiofilm efficacy. Together with the low toxicity, good safety profile and absence of any reported acquisition of bacterial resistance to PHMB, the antibiofilm effectiveness data support the alignment of this cleansing and irrigation solution with antimicrobial stewardship (AMS) strategies.

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Antibiofilm Properties of Antiseptic Agents Used on Pseudomonas aeruginosa Isolated from Diabetic Foot Ulcers

Cited by 11 publications

References 61 publications

Comparison of antibiofilm activity of low-concentrated hypochlorites vs polyhexanide-containing antiseptic

Comparison of antibiofilm activity of low-concentrated hypochlorites vs polyhexanide-containing antiseptic

The role of negative pressure wound therapy with instillation and dwell time in the treatment of deep sternal wound infections—A retrospective cohort study

Effectiveness of a polyhexamethylene biguanide-containing wound cleansing solution using experimental biofilm models

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