Antibiofilm surfaces based on the immobilization of a novel recombinant antimicrobial multidomain protein using self-assembled monolayers

Abstract

The constant increase of microorganisms resistant to antibiotics has been classified as a global health emergency, which is especially challenging when biofilms are formed. Here, novel biofunctionalized gold surfaces with...

Functionalization of Polydimethylsiloxane with Diazirine-Based Linkers for Covalent Protein Immobilization

“…Biosensors, such as immunosensors and enzyme electrodes, heavily rely on immobilized proteins and are extensively used for medical diagnostics, food analysis, and environmental monitoring . For biomaterials, immobilized biomolecules can be used to regulate cell adhesion, enhance device biocompatibility, and provide antifouling effects, including reducing microbial adhesion, among other applications. − For example, the immobilization of antimicrobial peptides and proteins onto biomaterial surfaces offers various advantages in preventing bacterial adhesion and biofilm-associated infections due to their wide range of activity, high efficacy, and low propensity for developing resistance. − Additionally, various microfluidic devices also require the attachment of proteins for applications in immunoassays, protein microarrays, cell studies, and functionalized devices such as blood oxygenators. , However, challenges still remain in ensuring consistent and stable activity of biomolecules on surfaces while also preventing their denaturation and undesirable interactions with the surface. , Furthermore, in order to obtain the greatest level of sensitivity and specificity in detection and response, a large amount of desired biomolecules need to be immobilized uniformly over the material with outward presentation of the active site . Various methods have been developed to improve biomolecule immobilization on solid surfaces, which can be divided into three categories: physical adsorption, electrostatic interaction, and covalent bonding. , The first two methods are relatively simple to apply; however, their effects are often temporary and reversible due to the dissociation of immobilized molecules from the surface. , Alternatively, covalent tethering with specific functional groups can be used for immobilization, and this binding process is usually irreversible due to the stable nature of the bond.…”