Antibiogram and Biofilm Development among Klebsiella pneumoniae from Clinical Isolates

Abstract

Objectives: This study was aimed to evaluate antibiotic resistance pattern and biofilm formation in K. pneumoniae strains isolated from different clinical specimens and to study on association of drug resistance pattern with biofilm formation. Methods: A total of 944 clinical samples from patients attending Sahid Gangalal National Heart Center were processed from September 2019 to March 2020 to identify possible bacterial pathogens following standard microbiological procedures. K. pneumonaie isolates were further subjected to antibiotic susceptibility testing using modified Kirby Bauer disc diffusion technique. Biofilm formation was evaluated by tissue culture plate technique. Results: Of the total 944 samples, 15.47% (146) samples showed bacterial growth, among which 23.97% (35) were K. pneumoniae. Out of 35 K. pneumoniae isolates, 45.71% (16) were multidrug-resistant and 42.86% (15) were extensively drug-resistant. Sixty percent (21) of K. pneumoniae feebly produced biofilm. Significant association was observed between biofilm production and exhibition of multidrug resistance (p value<0.05). Conclusion: Prevalence of antibiotics resistant K. pneumoniae in hospital setting is high and alarming. Significant association between drug resistance pattern and biofilm production implicates need of an immediate response to limit growth and spread of drug resistant microbes in clinical settings.

Identification and Characterization of Plasmids and Genes from Carbapenemase-Producing Klebsiella pneumoniae in Makkah Province, Saudi Arabia

“…The study showed that MEM was considered more effective than IMI, which 33% of samples resistant to IMI (MIC of >1024 µg/mL). These results are consistent with a study by Subash et al, who reported that the sensitivity of Gram-negative bacilli to MEM is more than IMI due to the overuse of the latter antibiotic (for many years) as an empirical treatment option [ 34 ].…”

Antibacterial and Antibiofilm Activity of the Mixed Lactobacilli Against the Most Bacterial Etiology of Otitis Media

“…Biofilms defined as bacterial aggregations attached to a surface and to each other and embedded in a self-produced structure composed of proteins (for example, fibrin), polysaccharides (for example, alginate), and extracellular DNA [6]. Biofilm is an important feature that aid pathogens avoiding host immune responses, survive at high levels of antibiotics, and establish a chronic infection.…”