Antibiograma rápido en Microbiología Clínica

Rosselló, Gabriel Alberto March; Pérez, María Jesús Verdú

doi:10.1016/j.eimc.2014.11.014

Cited by 13 publications

(4 citation statements)

References 87 publications

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“…It takes approximately 12 hr for a conventional PCR to perform, and this is partitioned into three important steps. The first step is DNA extraction, which is followed by the second step consisting of DNA amplification in a thermocycler, and then the detection of amplified DNA materials in gel electrophoresis (Rosselló & Pérez, 2016). this method is that you need to extract the DNA of so many different bacteria from different environmental sample matrices to be able to compare (Franklin, Aga, et al, 2016).…”

Section: Polymerase Chain Reactionmentioning

confidence: 99%

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The incidence of antibiotic resistance within and beyond the agricultural ecosystem: A concern for public health

2020

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The agricultural ecosystem creates a platform for the development and dissemination of antimicrobial resistance, which is promoted by the indiscriminate use of antibiotics in the veterinary, agricultural, and medical sectors. This results in the selective pressure for the intrinsic and extrinsic development of the antimicrobial resistance phenomenon, especially within the aquaculture‐animal‐manure‐soil‐water‐plant nexus. The existence of antimicrobial resistance in the environment has been well documented in the literature. However, the possible transmission routes of antimicrobial agents, their resistance genes, and naturally selected antibiotic‐resistant bacteria within and between the various niches of the agricultural environment and humans remain poorly understood. This study, therefore, outlines an overview of the discovery and development of commonly used antibiotics; the timeline of resistance development; transmission routes of antimicrobial resistance in the agro‐ecosystem; detection methods of environmental antimicrobial resistance determinants; factors involved in the evolution and transmission of antibiotic resistance in the environment and the agro‐ecosystem; and possible ways to curtail the menace of antimicrobial resistance.

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Section: Polymerase Chain Reactionmentioning

confidence: 99%

“…The MALDI-TOF MS identifies bacteria, yeast, and filamentous molds in a couple of minutes using protein analysis (Rosselló & Pérez, 2016). During antibiogram, MALDI-TOF MS predicts whether a bacteria harbors enzyme that hydrolyzes antibiotics, such as ESBLs and carbapenemases in approximately three hours.…”

Section: Maldi-tof Mass Spectrometrymentioning

confidence: 99%

The incidence of antibiotic resistance within and beyond the agricultural ecosystem: A concern for public health

2020

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“…In enterococci, seven Enterococcus faecalis and three Enterococcus faecium were tested against Ampicillin, Vancomycin and Ciprofloxacin at concentrations of 32, 16 The results of susceptibility obtained at 35 • C from colonies grown in culture plates by means of the commercial methods VITEK2 (bioMérieux, Marcy l'Etoile, France), MicroScan (Siemens, Tarrytown, NY, USA) and E-test (Liofilchem, Roseto Degli Abruzzi, Italy), applying the criteria published by the EUCAST, 9 were considered as the gold standard. Finally, results obtained by ATPbioluminescence were compared to the gold standard according to FDA criteria 10 ; agreements and disagreements among the susceptibility values obtained were classified as agreements, very major errors (false susceptibility), major errors (false resistance), or minor errors (susceptible/resistant versus intermediate susceptibility).…”

Section: Validation Proceduresmentioning

confidence: 99%

A two-hour antibiotic susceptibility test by ATP-bioluminescence

Rosselló

Urries

Rodríguez

et al. 2016

Enfermedades Infecciosas y Microbiología Clínica

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“…Several AST methods have been established, such as the disk diffusion method based on solid-plate AST, the broth dilution method for liquid AST, and polymerase chain reactionbased methods [7]. Recently, numerous rapid AST methods based on microfluidic systems with microscopic analysis have been developed to reduce the time for AST and to increase the accuracy, but the inoculum effects of those methods have not been studied [8][9][10][11][12].…”

Section: Introductionmentioning

confidence: 99%

Microscopic Analysis of Bacterial Inoculum Effect Using Micropatterned Biochip

2021

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Antimicrobial resistance has become a major problem in public health and clinical environments. Against this background, antibiotic susceptibility testing (AST) has become necessary to cure diseases in an appropriate and timely manner as it indicates the necessary concentration of antibiotics. Recently, microfluidic based rapid AST methods using microscopic analysis have been shown to reduce the time needed for the determination of the proper antibiotics. However, owing to the inoculum effect, the accurate measurement of the minimal inhibitory concentration (MIC) is difficult. We tested four standard bacteria: Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, and Enterococcus faecalis, against five different antibiotics: piperacillin, cefotaxime, amikacin, levofloxacin, and ampicillin. The results showed that overall, the microfluidic system has a similar inoculum effect compared to the conventional AST method. However, due to the different testing conditions and determination protocols of the growth of the microfluidic based rapid AST, a few results are not identical to the conventional methods using optical density. This result suggests that microfluidic based rapid AST methods require further research on the inoculum effect for practical use in hospitals and can then be used for effective antibiotic prescriptions.

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Antibiograma rápido en Microbiología Clínica

Cited by 13 publications

References 87 publications

The incidence of antibiotic resistance within and beyond the agricultural ecosystem: A concern for public health

The incidence of antibiotic resistance within and beyond the agricultural ecosystem: A concern for public health

A two-hour antibiotic susceptibility test by ATP-bioluminescence

Microscopic Analysis of Bacterial Inoculum Effect Using Micropatterned Biochip

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