Antibiotic and mammal IgG based lateral flow assay for simple and sensitive detection of Staphylococcus aureus

Zhao, Man; Yao, Xiaolin; Liu, Sijie; Zhang, Han; Wang, Lulu; Yin, Xuechi; Su, Lihong; Xu, Baocheng; Wang, Jianlong; Lan, Quanxue; Zhang, Daohong

doi:10.1016/j.foodchem.2020.127955

Cited by 47 publications

(14 citation statements)

References 36 publications

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“…In brief, three factors must have been highlighted: (i) in comparison with Ab molecules, the spectral detection reagent, VAN, has demonstrated leading superiorities such as low cost, easy accessibility, the ability to control the quality, and high stability; (ii) using commercial pig IgG as a detection reagent has not only demonstrated satisfactory specificity but also has avoided the slow and labor‐intensive monitoring of anti‐ S. aureus Abs; (iii) the fabricated sandwich procedure has a favorable LOD of 10 3 CFU/mL and has shown an acceptable anti‐interference presentation and high reliability for S. aureus recognition. As predicted, the settled VAN‐Au NPs based on sandwich LFA could have been possessed as a powerful screening device for monitoring S. aureus contamination in food safety and the environment (Zhao et al., 2021).…”

Section: Staphylococcus Aureusmentioning

confidence: 93%

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State of the art: Lateral flow assays toward the point‐of‐care foodborne pathogenic bacteria detection in food samples

Sohrabi

Majidi

Khaki

et al. 2022

Comp Rev Food Sci Food Safe

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Diverse chemicals and some physical phenomena recently introduced in nanotechnology have enabled scientists to develop useful devices in the field of food sciences. Concerning such developments, detecting foodborne pathogenic bacteria is now an important issue. These kinds of bacteria species have demonstrated severe health effects after consuming foods and high mortality related to acute cases. The most leading path of intoxication and infection has been through food matrices. Hence, quick recognition of foodborne bacteria agents at low concentrations has been required in current diagnostics. Lateral flow assays (LFAs) are one of the urgent and prevalently applied quick recognition methods that have been settled for recognizing diverse types of analytes. Thus, the present review has stressed on latest developments in LFAs‐based platforms to detect various foodborne pathogenic bacteria such as Salmonella, Listeria, Escherichia coli, Brucella, Shigella, Staphylococcus aureus, Clostridium botulinum, and Vibrio cholera. Proper prominence has been given on exactly how the labels, detection elements, or procedures have affected recent developments in the evaluation of diverse bacteria using LFAs. Additionally, the modifications in assays specificity and sensitivity consistent with applied food processing techniques have been discussed. Finally, a conclusion has been drawn for highlighting the main challenges confronted through this method and offered a view and insight of thoughts for its further development in the future.

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Section: Staphylococcus Aureusmentioning

confidence: 93%

“…Adapted by permission from Zhao et al. (2021). (d) Graphic demonstration of POCT of 16S rRNA of S. aureus founded on multiple biotin‐labeled DNA probes, LFA, and smartphone (Zheng et al., 2019).…”

Section: Staphylococcus Aureusmentioning

confidence: 99%

State of the art: Lateral flow assays toward the point‐of‐care foodborne pathogenic bacteria detection in food samples

Sohrabi

Majidi

Khaki

et al. 2022

Comp Rev Food Sci Food Safe

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Section: Resultsmentioning

confidence: 99%

“…Vancomycin (Van) is a kind of glycopeptide antibiotic and can selectively interact with Gram-positive bacteria, e.g., S. aureus, via a hydrogen bonding effect using its D-Ala-D-Ala-termini. 46 Based on this specific recognition, FCNBs are covalently modified with vancomycin to form the label probe, i.e., FCNBs-Van (details seen in the SI). The label probe would then bind with S. aureus and the antibody fixed at the T line to construct antibody−target−probe sandwich complexes, resulting in the generation of fluorescence at the T line.…”

Section: ■ Experimental Sectionmentioning

confidence: 99%

An Emerging Fluorescent Carbon Nanobead Label Probe for Lateral Flow Assays and Highly Sensitive Screening of Foodborne Toxins and Pathogenic Bacteria

Deng

Qian

et al. 2022

Anal. Chem.

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By virtue of the fascinating merits of low cost, rapid screening, and on-site detection, fluorescence lateral flow assays (FLFAs) have attracted considerable attention. Their detection limits are closely associated with the label probes used. The development of high-performance and robust phosphors remains a great challenge. Herein, we presented a new label probe, i.e., fluorescent carbon nanobeads (FCNBs), for FLFAs. Monodispersive, water-soluble, and highly emissive FCNBs were facilely prepared via a hydrothermal carbonization manner. Their abundant amino groups were beneficial for versatile surface functionalization. After being modified by biomolecules, the fabricated FCNB reporter probes were adopted for the construction of lateral flow test strips toward representative foodborne toxins, i.e., aflatoxin B1 (AFB1), and pathogenic bacteria, i.e., Staphylococcus aureus (S. aureus), respectively. The detection limits (0.01 ng/mL for AFB1 and 102 cfu/mL for S. aureus) were about 1 or 2 orders of magnitude lower than most reported methods. Furthermore, the proposed test strips were successfully applied for the quantitative, accurate, and rapid screening of AFB1 and S. aureus in food samples. This work provided a promising label probe for FLFAs and would open the opportunity to exploit a sensing platform by modifying different ligands onto the FCNBs.

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“…Although the molecularly imprinted polymer technique possessed a short detection time, it had lower sensitivity and a narrow linear range . Antibody-based immunoassay methods exhibited high specificity for the target, yet possess higher LOD values. − These methods also involve complications in antibody labeling, which are expensive and highly vulnerable to external environmental influences. To enhance the analytical performance, our research group previously developed a SERS aptasensor based on a AuNP-functionalized PDMS film for sensitive determination of S.…”

Section: Results and Discussionmentioning

confidence: 99%

Upconversion Nanoprobes Based on a Horseradish Peroxidase-Regulated Dual-Mode Strategy for the Ultrasensitive Detection of Staphylococcus aureus in Meat

Ouyang

Wang

Ahmad

et al. 2021

J. Agric. Food Chem.

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Staphylococcus aureus (S. aureus) is one of the foodborne pathogens that can cause infectious diseases and food poisoning. Herein, colorimetric and fluorescent dual-mode nanoprobes were developed for ultrasensitive detection of S. aureus to immediately respond to public health emergencies, reduce false positives, and improve measurement accuracy and persuasiveness. The nanoprobe consists of aptamer-labeled magnetic nanoparticles (apt-MNPs) as the capture signal probe and horseradish peroxidase and complementary DNA-functionalized upconversion nanoparticles (HRP-UCNPs-cDNA) as the chromogenic signal probe. In the absence of S. aureus, the probe forms an immune complex through base complementation with an observable signal. When S. aureus is introduced to this system, it preferentially binds to the apt-MNPs, releasing HRP-UCNPs-cDNA from the apt-MNPs and restoring the chromogenic probe signal. Under optimum conditions, an ultrasensitive assay of S. aureus was obtained, with limits of detection of 22 CFU mL–1 for fluorescence and 20 CFU mL–1 for colorimetry in a linear range of 56–5.6 × 106 CFU mL–1. Additionally, the standard plate counting method confirmed the reliability and accuracy of the established nanoprobe with an insignificant difference. Hence, the developed dual-mode platform has extensive application prospects for speedy and specific determination of S. aureus in meat.

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Antibiotic and mammal IgG based lateral flow assay for simple and sensitive detection of Staphylococcus aureus

Cited by 47 publications

References 36 publications

State of the art: Lateral flow assays toward the point‐of‐care foodborne pathogenic bacteria detection in food samples

State of the art: Lateral flow assays toward the point‐of‐care foodborne pathogenic bacteria detection in food samples

An Emerging Fluorescent Carbon Nanobead Label Probe for Lateral Flow Assays and Highly Sensitive Screening of Foodborne Toxins and Pathogenic Bacteria

Upconversion Nanoprobes Based on a Horseradish Peroxidase-Regulated Dual-Mode Strategy for the Ultrasensitive Detection of Staphylococcus aureus in Meat

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