Antibiotic-decorated titanium with enhanced antibacterial activity through adhesive polydopamine for dental/bone implant

Abstract

Implant-associated infections, which are normally induced by microbial adhesion and subsequent biofilm formation, are a major cause of morbidity and mortality. Therefore, practical approaches to prevent implant-associated infections are in great demand. Inspired by adhesive proteins in mussels, here we have developed a novel antibiotic-decorated titanium (Ti) material with enhanced antibacterial activity. In this study, Ti substrate was coated by one-step pH-induced polymerization of dopamine followed by immobilization of the antibiotic cefotaxime sodium (CS) onto the polydopamine-coated Ti through catechol chemistry. Contact angle measurement and X-ray photoelectron spectroscopy confirmed the presence of CS grafted on the Ti surface. Our results demonstrated that the antibiotic-grafted Ti substrate showed good biocompatibility and well-behaved haemocompatibility. In addition, the antibiotic-grafted Ti could effectively prevent adhesion and proliferation of Escherichia coli (Gram-negative) and Streptococcus mutans (Gram-positive). Moreover, the inhibition of biofilm formation on the antibiotic-decorated Ti indicated that the grafted CS could maintain its long-term antibacterial activity. This modified Ti substrate with enhanced antibacterial activity holds great potential as implant material for applications in dental and bone graft substitutes.

Tough, self-healable and tissue-adhesive hydrogel with tunable multifunctionality

“…It has been reported that the catechol/quinone groups on PDA could crosslink with nucleophilic amine groups via a Schiff base reaction. 28,30,31 In agreement with this notion, the tentative assignment of the peak at m/z 261 is C 13 H 14 N 2 O 4 + (see the inset of Figure 2b) that results from interactions between the amine groups of PAM and the catechol groups of PDA. This reaction connects the PDA chains to the PAM networks.…”

Biofunctionalization of titanium with bacitracin immobilization shows potential for anti-bacteria, osteogenesis and reduction of macrophage inflammation

“…In the present study, the late markers of osteogenic differentiation (Alizarin Red staining) could be observed on the Ti-BC surface within 2 weeks. In contrast to other antibiotics immobilized on the dopamine coated Ti, which could be only able to enhance antibacterial activity [37]. The bacitracin modified Ti surface could not only exhibit the antibacterial properties but also promote the osteogenic differentiation, which was of great importance to prevent the orthopedic implant infection.…”

Antimicrobial dental implant functionalization strategies —A systematic review

“…An interactive implant surface with pH-dependent or infection-dependent antibiotic release might avoid this shortcoming, but has not yet been introduced to dental implant materials research. Antibiotics are capable of reducing bacterial colonization with S. mutans 22,39) S. epidermidis 31) and S.aureus 38) , P. gingivalis, A. actinomycetemcomitans, P. intermedia, P. aeruginosa 40) and E. coli 21,23,36) on titanium surfaces. Chlorhexidine (CHX)-loaded titanium surfaces reduce colonization with streptococci and S. aureus in comparison to titanium control [24][25][26] .…”