Antibiotic depot system with radiofrequency controlled drug release

Navrátil, Ondřej; Lizoňová, Denisa; Slonková, Karolína; Mašková, Lucie; Zadražil, Aleš; Sedmidubský, David; Štěpánek, František

doi:10.1016/j.colsurfb.2022.112618

Cited by 2 publications

(3 citation statements)

References 23 publications

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“…No free IONs could be detected in the supernatant, indicating that the embedding of IONs in the polysaccharide shell of mGPs was sufficiently strong to prevent the loss of magnetic properties over time. The full characterization of the magnetic properties of IONs including magnetization curves at 5 and 300 K and field-cooled and zero-field-cooled susceptibility have been reported in our recent work . The macroscopic manifestation of their magnetic properties is the ability to attract mGPs to a permanent magnet and separate them from solution, as shown in Figure .…”

Section: Resultsmentioning

confidence: 84%

“…The full characterization of the magnetic properties of IONs including magnetization curves at 5 and 300 K and field-cooled and zero-field-cooled susceptibility have been reported in our recent work. 29 The macroscopic manifestation of their magnetic properties is the ability to attract mGPs to a permanent magnet and separate them from solution, as shown in Figure 2 .…”

Section: Resultsmentioning

confidence: 99%

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Magnetic Yeast Glucan Particles for Antibody-Free Separation of Viable Macrophages from Drosophila melanogaster

Krejčová,

Saloň,

Klimša

et al. 2023

ACS Biomater. Sci. Eng.

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Currently available methods for cell separation are generally based on fluorescent labeling using either endogenously expressed fluorescent markers or the binding of antibodies or antibody mimetics to surface antigenic epitopes. However, such modification of the target cells represents potential contamination by non-native proteins, which may affect further cell response and be outright undesirable in applications, such as cell expansion for diagnostic or therapeutic applications, including immunotherapy. We present a label-and antibody-free method for separating macrophages from living Drosophila based on their ability to preferentially phagocytose whole yeast glucan particles (GPs). Using a novel deswelling entrapment approach based on spray drying, we have successfully fabricated yeast glucan particles with the previously unachievable content of magnetic iron oxide nanoparticles while retaining their surface features responsible for phagocytosis. We demonstrate that magnetic yeast glucan particles enable macrophage separation at comparable yields to fluorescence-activated cell sorting without compromising their viability or affecting their normal function and gene expression. The use of magnetic yeast glucan particles is broadly applicable to situations where viable macrophages separated from living organisms are subsequently used for analyses, such as gene expression, metabolomics, proteomics, single-cell transcriptomics, or enzymatic activity analysis.

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Section: Resultsmentioning

confidence: 84%

Section: Resultsmentioning

confidence: 99%

Magnetic Yeast Glucan Particles for Antibody-Free Separation of Viable Macrophages from Drosophila melanogaster

Krejčová,

Saloň,

Klimša

et al. 2023

ACS Biomater. Sci. Eng.

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“…Controlled release of active ingredients through particles and carriers, triggered by stimuli like temperature, pH, magnetic and electric fields, is gaining popularity in pharmaceuticals 16 , 17 , cosmetics (skincare products 18 ), and agro-industries (smart fertilisers 19 ). Encapsulating antimicrobial agents, such as synthetic and natural antibiotics 20 , enzymes or inorganic nanoparticles 21 , play an important role in treating or preventing infections.…”

Section: Introductionmentioning

confidence: 99%

Development of spray-dried powder hand sanitiser with prolonged effectivity

Večerková,

Mašková,

Knejzlík

et al. 2024

Sci Rep

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Since the outbreak of the COVID-19 pandemic, the use of hand sanitisers has become an inseparable part of our personal hygiene. However, the short-term effect and the need for frequent application are shortcomings that impair the overall protection. Another aspect is that repeated use of some products (typically alcohol-based) may cause skin irritation or eventually more severe health problems. This work proposes spray-drying as a suitable method for the preparation of swellable chitosan carriers, allowing for encapsulation and sustained release of antibacterial chlorhexidine digluconate as a model active substance. After application to hands, micron-sized particles preferentially accommodate space between epidermal ridges, protected against attrition. Thanks to their small size (d < 10 µm), particles are comfortable to carry since they are not recognisable by somatosensory receptors. The performance of formulations with various amounts of chlorhexidine and cross-linker was tested and compared with selected commercial disinfectants available on the Czech market (ethanol gel and alcoholic solution with chlorhexidine) against E.coli and S.epidermidis. The real-life performance was investigated with twelve volunteers performing various activities for up to 2 h. Finally, a replica of the human index finger with accurately captured micro-topology was proposed and compared with volunteers’ fingers concerning the total amount of adhered and detached particles.

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Antibiotic depot system with radiofrequency controlled drug release

Cited by 2 publications

References 23 publications

Magnetic Yeast Glucan Particles for Antibody-Free Separation of Viable Macrophages from Drosophila melanogaster

Magnetic Yeast Glucan Particles for Antibody-Free Separation of Viable Macrophages from Drosophila melanogaster

Development of spray-dried powder hand sanitiser with prolonged effectivity

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