Antibiotic Resistant Bacteria - A Continuous Challenge in the New Millennium 2012
DOI: 10.5772/28510
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Antibiotic-Free Selection for Bio-Production: Moving Towards a New "Gold Standard"

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Cited by 9 publications
(13 citation statements)
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References 31 publications
(26 reference statements)
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“…Os crescentes requerimentos regulatórios aos quais agentes biológicos estão sujeitos terão um grande impacto no campo da expressão e produção industrial de proteínas. Há uma expectativa de que, num futuro próximo, haja "tolerância zero" em relação aos sistemas de produção e seleção baseados em antibióticos (SODOYER et al, 2012).…”
Section: 52unclassified
“…Os crescentes requerimentos regulatórios aos quais agentes biológicos estão sujeitos terão um grande impacto no campo da expressão e produção industrial de proteínas. Há uma expectativa de que, num futuro próximo, haja "tolerância zero" em relação aos sistemas de produção e seleção baseados em antibióticos (SODOYER et al, 2012).…”
Section: 52unclassified
“…However, there are several limitations associated with the use of E. coli expression system for the production of recombinant proteins for commercial and biomedical applications. Instability of recombinant plasmids (carrying the gene-encoding target recombinant protein) during high-density cultivation of host cells is known to result in low yield of the target recombinant proteins (Peubez et al 2010;Sodoyer et al 2012). In addition, the use of antibiotics during the cultivation of host cells (recombinant E. coli cells) expressing target recombinant proteins is a major concern (Peubez et al 2010;Sodoyer et al 2012).…”
Section: Reference Of Submitted Sequencesmentioning
confidence: 99%
“…We propose to replace this resistance to an antibiotic by the Staby ® technology based on natural poison/antidote bacterial genes to insure the plasmid retention during cloning and DNA production. [10][11][12] There is a large number of poison/antidote systems, including in bacteria used industrially for production of proteins used as medical products (antigen vaccines, etc). [13][14][15][16][17] Among these systems, there is the ccd system composed of the ccdA (antidote) and ccdB genes (poison).…”
Section: Introductionmentioning
confidence: 99%