Antibiotic-induced dysbiosis of the rat oral and gut microbiota and resistance to Salmonella

Huang, Wu; Ma, Yue; Peng, Xian; Qiu, Wei; Kong, Lixin; Ren, Biao; Li, Mingyun; Cheng, Guo; Zhou, Xuedong; Cheng, Lei

doi:10.1016/j.archoralbio.2020.104730

Cited by 28 publications

(24 citation statements)

References 48 publications

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“…Therefore, we comprehensively demonstrated the different oral microbiota between H. pylori-infected individuals and normal controls using diversity, Antibiotics and proton-pump inhibitors are the main components in the treatment of H. pylori infection and could also cause disturbances in the host microbiota in the short-term follow-up. [26][27][28] BQT could influence the gut microbiota after eradication, and the alterations recovered after a long-term period. 15,29 The oral microbiota was also confirmed to be altered 2 months after BQT, as revealed by diversity and compositional analysis.…”

Section: Discussionmentioning

confidence: 99%

Analysis of oral microbiota alterations induced by Helicobacter pylori infection and vonoprazan‐amoxicillin dual therapy for Helicobacter pylori eradication

Ouyang

et al. 2022

Helicobacter

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Background The oral cavity is considered a potential reservoir of Helicobacter pylori (H. pylori), and the imbalance of oral microbiota directly reflects the health of the host. We aimed to explore the relationship among oral microbiota, H. pylori infection, and vonoprazan‐amoxicillin (VA) dual therapy for H. pylori eradication. Methods Helicobacter pylori‐positive patients were randomized into low‐ or high‐dose VA dual therapy (i.e., amoxicillin 1 g b.i.d. or t.i.d. and vonoprazan 20 mg b.i.d) for 7 or 10 days. H. pylori‐negative patients served as normal controls. Saliva samples were collected from 41 H. pylori‐positive patients and 13 H. pylori‐negative patients. The oral microbiota was analyzed by 16S rRNA gene sequencing, followed by bioinformatics analysis. Results Helicobacter pylori‐positive patients had higher richness and diversity and better evenness of oral microbiota than normal controls. Beta diversity analysis estimated by Bray–Curtis or weighted UniFrac showed distinct clustering between H. pylori‐positive patients and normal controls. The number of bacterial interactions was reduced in H. pylori‐positive patients compared with that in negative patients. Forty‐one patients evaluated before and after successful H. pylori eradication were divided into low (L‐VA) and high dose (H‐VA) amoxicillin dose groups. The alpha and beta diversity of the oral microbiota between L‐VA and H‐VA patients exhibited no differences at the three time points (before eradication, after eradication, and at confirmation of H. pylori infection cure). Conclusion Helicobacter pylori infection could alter the diversity, composition, and bacterial interactions of the oral microbiota. Both L‐VA and H‐VA dual therapy showed minimal influence on the oral microbiota.

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Section: Discussionmentioning

confidence: 99%

Analysis of oral microbiota alterations induced by Helicobacter pylori infection and vonoprazan‐amoxicillin dual therapy for Helicobacter pylori eradication

Ouyang

et al. 2022

Helicobacter

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“…Because of these important roles, an imbalance in the oral microbiome or dysbiosis within these oral biofilms is associated with a variety of oral and systemic diseases, including Alzheimer’s disease, head and neck cancer, periodontal disease, caries, and peri-implantitis [ 11 , 15 , 18 ]. Although antibiotics can be used to address this dysbiosis, recent data indicate that antibiotics may even induce dysbiosis in such a way that the oral microbiota cannot recover [ 26 , 27 ]. Peri-implant diseases are in part biofilm mediated, and exacerbation of disease can lead to loss of implants over time.…”

Section: Discussionmentioning

confidence: 99%

“…Thus, there is currently no major consensus on the best treatment modality for peri-implant disease [ 25 ]. Additionally, the use of local or systemic antibiotics may also induce further dysbiosis in oral and gut microbiomes, and both microbiotas may not be able to recover [ 26 , 27 ]. Therefore, addressing the etiology of peri-implant disease is paramount to the successful resolution of this situation, also further highlighting the urgent need for new treatment strategies.…”

Section: Introductionmentioning

confidence: 99%

Nisin and Nisin Probiotic Disrupt Oral Pathogenic Biofilms and Restore Their Microbiome Composition towards Healthy Control Levels in a Peri-Implantitis Setting

et al. 2022

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Peri-implantitis is characterized by chronic inflammation of the peri-implant supporting tissues that progressively and irreversibly leads to bone loss and, consequently, implant loss. Similar to periodontal disease, oral dysbiosis is thought to be a driver of peri-implantitis. However, managing peri-implantitis with traditional treatment methods, such as nonsurgical debridement or surgery, is not always successful. Thus, novel strategies have been proposed to address these shortcomings. One strategy is the use of probiotics as antimicrobial agents since they are considered safe for humans and the environment. Specifically, the probiotic Lactococcus lactis produces nisin, which has been used worldwide for food preservation. The objective of this study was to determine whether nisin and the wild-type (WT) nisin-producing L. lactis probiotic can disrupt oral pathogenic biofilms and promote a healthier oral microbiome within these oral biofilms on titanium discs. Using confocal imaging and 16S rRNA sequencing, this study revealed that nisin and WT L. lactis probiotic disrupt oral pathogenic biofilms in a peri-implantitis setting in vitro. More specifically, nisin decreased the viability of the pathogen-spiked biofilms dose-dependently from 62.53 ± 3.69% to 54.26 ± 3.35% and 44.88 ± 2.98%, respectively. Similarly, 105 CFU/mL of WT L. lactis significantly decreased biofilm viability to 52.45 ± 3.41%. Further, both treatments shift the composition, relative abundance, and diversity levels of these biofilms towards healthy control levels. A total of 1 µg/mL of nisin and 103 CFU/mL of WT L. lactis were able to revert the pathogen-mediated changes in the Proteobacteria (from 80.5 ± 2.9% to 75.6 ± 2.0%, 78.0 ± 2.8%, and 75.1 ± 5.3%, respectively) and Firmicutes (from 11.6 ± 1.6% to 15.4 ± 1.3%, 13.8 ± 1.8%, and 13.7 ± 2.6%, respectively) phyla back towards control levels. Thus, nisin and its nisin-producing L. lactis probiotic may be useful in treating peri-implantitis by promoting healthier oral biofilms, which may be useful for improving patient oral health.

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“…Moreover, frequent exposure of the pathogenic bacteria to antibiotics could lead to the antibiotic resistance crisis (Ventola, 2015;Yelin and Kishony, 2018). Worse still, most recent researches revealed that the drug-resistant pathogens could further promote the spread of resistant plasmid in gut and then induce secondary infection (Bakkeren et al, 2019;Wu et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Comparison of the Gut Microbiota Between Pulsatilla Decoction and Levofloxacin Hydrochloride Therapy on Escherichia coli Infection

Liu

et al. 2020

Front. Cell. Infect. Microbiol.

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Gut microbiota serves as a critical indicator for gut health during treatment of pathogenic bacterial infection. Both Pulsatilla Decoction (abbreviated to PD, a traditional Chinese medicine compound) and Levofloxacin Hydrochloride (LVX) were known to have therapeutic effects to intestinal infectious disease. However, the changes of gut microbiota after PD or LVX treatment remain unclear. Herein, this work aimed to investigate the changes of intestinal flora after PD or LVX therapy of Escherichia coli infection in rats. Results revealed that PD exhibited a valid therapeutic approach for E. coli infection via the intestinal protection, as well as the inhibited release of IL-8 and ICAM-1. Besides, PD was beneficial to rebuild the gut microbiota via restoring Bacteroidetes spp in the composition of the gut microbiota. Comparatively, LVX treatment promoted the infection and ravaged gut microbiota by significantly decreasing Bacteroidetes and increasing Firmicutes. These findings not only highlight the mechanism of Chinese herbal formula, but extend the application of PD as veterinary medicine, feed additive and pre-mixing agent for improving the production of animal derived foods.

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Antibiotic-induced dysbiosis of the rat oral and gut microbiota and resistance to Salmonella

Cited by 28 publications

References 48 publications

Analysis of oral microbiota alterations induced by Helicobacter pylori infection and vonoprazan‐amoxicillin dual therapy for Helicobacter pylori eradication

Analysis of oral microbiota alterations induced by Helicobacter pylori infection and vonoprazan‐amoxicillin dual therapy for Helicobacter pylori eradication

Nisin and Nisin Probiotic Disrupt Oral Pathogenic Biofilms and Restore Their Microbiome Composition towards Healthy Control Levels in a Peri-Implantitis Setting

Comparison of the Gut Microbiota Between Pulsatilla Decoction and Levofloxacin Hydrochloride Therapy on Escherichia coli Infection

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