Antibiotic Inhibition of the Septation Stage in Sporulation of
            <i>Bacillus megaterium</i>

Hitchins, Anthony D.; Slepecky, Ralph A.

doi:10.1128/jb.97.3.1513-1515.1969

Cited by 24 publications

(20 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thermosensitive blocking of cell division septation and sporulation septation in the same mutant suggested that the two processes were closely related (7). Such a relationship supports the hypothesis that the early stages of sporulation (Stages I and II) represent a modified cell division (8).…”

supporting

confidence: 64%

“…The parent strain was Bacillus megaterium ATCC 19213. The mutant strain (TH14) was derived from the parent strain by chemical mutagenesis (7). Stock cultures were maintained in the sporulated state (7).…”

Section: Methodsmentioning

confidence: 99%

“…Previously described techniques. Determinations of heat-resistant colony-forming units, percentage of sporulation and DNA biosynthesis, and the preparation of ultrathin sections for electron microscopy have been described (7).…”

Section: Methodsmentioning

confidence: 99%

“…The other, which is the subject of this report, is a ts mutant (TH14) of Bacillus megaterium that grows normally at 30 C but forms aseptate filaments at 39 C, the restrictive temperature. This mutant is also ts asporogenous being blocked at sporulation Stage I (DNA in axial filament configuration) since no sporulation septa (Stage II) are detectable at the restrictive temperature (7). The apparent lack of recognizable Stage I forms in the mutant at the restrictive temperature can be attributed to the difficulty of defining the stage in the parent strain (3).…”

mentioning

confidence: 99%

See 3 more Smart Citations

Properties of a Thermosensitive Asporogenous Filamentous Mutant of Bacillus megaterium

Hitchins

Sadoff

1974

J Bacteriol

Self Cite

View full text Add to dashboard Cite

Bacillus megaterium ATCC 19213 is thermosensitive and defective in cell-division septation and spore formation at the restrictive temperature (39 C). As a consequence, the mutant forms multinucleate aseptate filaments and is asporogenic. The mutation does not result in any qualitative compositional changes in extractable membrane proteins. At the restrictive temperature, the mutant membrane has a reduced content of a small molecular weight protein(s). A membrane protein(s) with a molecular weight of nearly 80,000 appears to be partially derepressed in the mutant grown at the restrictive temperature. In addition, numerous unidentified spherical inclusions of fairly uniform size (diameter approximately 100 nm) are present in the cytoplasm at the restrictive temperature. They are especially concentrated at only one pole of each filament. Filamentous growth of the mutant is less sensitive to penicillin than growth in the rod form. Growth in either form is equally sensitive to D-cycloserine at the concentrations used for selection of the mutant. Temperature shift-up experiments suggest that one to two rounds of deoxyribonucleic acid (DNA) replication occur before the phenotypic expression of the mutation occurs. The septations after these replication events can be either two-division septations or a single-division septation plus a subsequent sporulation septation. This conclusion, coupled with previously reported work, supports the hypothesis that the early stages of sporulation represent a modified cell division. Certain thermal-sensitive (ts) mutants have 'Journal article no. 6050 from the Michigan Agricultural Experiment Station.

show abstract

supporting

confidence: 64%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Properties of a Thermosensitive Asporogenous Filamentous Mutant of Bacillus megaterium

Hitchins

Sadoff

1974

J Bacteriol

Self Cite

View full text Add to dashboard Cite

show abstract

“…It has generally been thought that the prespore septum differs from the normal division septum in that it contains no cell wall material (15,23). The distinction is now questionable.…”

mentioning

confidence: 99%

Requirement for peptidoglycan synthesis during sporulation of Bacillus subtilis

Dancer¹

1979

J Bacteriol

View full text Add to dashboard Cite

Cultures of Bacillus subtilis were treated during sporulation with antibiotics (bacitracin and vancomycin) that affect peptidoglycan synthesis. The cells were resistant to the effects of the antibiotics only when the drugs were added about 2 h after the beginning of sporulation. This was about 1 h later than the escape time of a temperature-sensitive sporulation mutant that is unable to complete prespore septation. Similar experiments were done with a mutant temperature sensitive for peptidoglycan synthesis. This showed an escape curve similar to that shown by the antibiotics. When sporulating cells were treated with antibiotics, they produced alkaline phosphatase earlier than normal. Enzyme production was unaffected by inhibition of deoxyribonucleic acid synthesis but was inhibited by chloramphenicol. Sporulation mutants that are unable to make alkaline phosphatase under normal conditions were able to make it in the presence of bacitracin. The alkaline phosphatase made under these conditions was under "sporulation-type" control since its synthesis was repressible by casein hydrolysate and unaffected by inorganic phosphate. When cells were treated with bacitracin in the growth medium as well as in the sporulation medium, alkaline phosphatase synthesis was at the same level as in an untreated control. A number of other antibiotics and surfactants were tested for the ability to cause premature production of the phosphatase of those tested, only taurodeoxycholate whowed this behavior. Moreover, incubation of cells with taurodeoxycholate in the growth medium as well as in the sporulation medium prevented premature enzyme production.

show abstract

Reversion sporulierender Bacillus megaterium‐Zellen zum Wachstum

Kretschmer¹

1972

J of Basic Microbiology

View full text Add to dashboard Cite

In carbohydrate-limited, sporulating B. megaterium cultures reversion t o growth was initiated by transfer into medium containing glucose and yeast-extract. From cultures grown with different doubling times the iknetics of commitment to sporulation and loss of reversibility were studied both by phase-contrast and electron microscopy. Commitment to refractility occurred about 20 min after formation of the spore septum and 140 min before spores became refractil. Not all of the cells which did not reach this stage of commitment until the time of transfer were able to revert. Reversibility was already lost during formation of the spore septum. Stage 11-cells which had formed the spore septum before transfer were committed to stage I11 despite the presence of nutrients, but their further development was blocked. Thus, like in other spore-formers the condition for undisturbed spore development was the formation of the forespore under starvation. As only stage I-cells were able to undergo reversion to growth, commitment to refractility did not correspond to loss of reversibility, as could be shown with other spore-formers.Before in reverting cells the first division septum appeared (about one hr after transfer) the cells doubled in length. During that time in about 60% of the cells additional wall material was laid down a t the younger poles. The first division septum was not formed in the middle of the cell, but the part with the older pole which would have built the spore was larger. In the presence of penicillin G the parts of the reverting cells were influenced differently. The part containing the younger pole got thicker, while the other showed the normal diameter.

show abstract

Antibiotic Inhibition of the Septation Stage in Sporulation of Bacillus megaterium

Cited by 24 publications

References 9 publications

Properties of a Thermosensitive Asporogenous Filamentous Mutant of Bacillus megaterium

Properties of a Thermosensitive Asporogenous Filamentous Mutant of Bacillus megaterium

Requirement for peptidoglycan synthesis during sporulation of Bacillus subtilis

Reversion sporulierender Bacillus megaterium‐Zellen zum Wachstum

Contact Info

Product

Resources

About