2021
DOI: 10.1038/s41598-020-80764-6
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Antibiotic loaded β-tricalcium phosphate/calcium sulfate for antimicrobial potency, prevention and killing efficacy of Pseudomonas aeruginosa and Staphylococcus aureus biofilms

Abstract: This study investigated the efficacy of a biphasic synthetic β-tricalcium phosphate/calcium sulfate (β-TCP/CS) bone graft substitute for compatibility with vancomycin (V) in combination with tobramycin (T) or gentamicin (G) evidenced by the duration of potency and the prevention and killing efficacies of P. aeruginosa (PAO1) and S. aureus (SAP231) biofilms in in vitro assays. Antibiotic loaded β-TCP/CS beads were compared with antibiotic loaded beads formed from a well characterized synthetic calcium sulfate (… Show more

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Cited by 16 publications
(15 citation statements)
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“…16,19 Similar to our results, recent studies have shown that the elution rates of antibiotics from calcium sulfate are successful at eradicating bacteria in surrounding tissues over time due to the initial high local antibiotic release, but this success occurs during a limited period of time due to the biodegradable properties of the antibiotics. 24,26 Nevertheless, many studies have only tested sensitive ATCC strains. 19 In addition to ATCC strains, we obtained more information by selecting well-investigated MDR staphylococcal clinical strains recovered from sonication fluid, and WGS analysis was performed to ensure that both strains exhibited resistance, MSCRAMMs (microbial surface components recognizing adhesive matrix molecules), and other biofilm-forming genes.…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…16,19 Similar to our results, recent studies have shown that the elution rates of antibiotics from calcium sulfate are successful at eradicating bacteria in surrounding tissues over time due to the initial high local antibiotic release, but this success occurs during a limited period of time due to the biodegradable properties of the antibiotics. 24,26 Nevertheless, many studies have only tested sensitive ATCC strains. 19 In addition to ATCC strains, we obtained more information by selecting well-investigated MDR staphylococcal clinical strains recovered from sonication fluid, and WGS analysis was performed to ensure that both strains exhibited resistance, MSCRAMMs (microbial surface components recognizing adhesive matrix molecules), and other biofilm-forming genes.…”
Section: Discussionmentioning
confidence: 99%
“…The antimicrobial elution method established for biofilm death (time kill curve) was based on that of Jiang et al 26 PMMA, PG-CSH and unloaded beads (5 beads per well) were placed in a 24-well tissue culture plate (Sarstedt, Newton, United States). Each well was inoculated with 2 mL of tryptone soya broth (TSB, Oxoid, Hampshire, United Kingdom) enriched with 1% glucose that contained 10 6 CFU/ mL of the bacterial strains (ATCC29213, ATCC12228, SA260, and SEPI216).…”
Section: Pg-csh and Pmma Beads With Antimicrobial Elution To Effectiv...mentioning
confidence: 99%
“…A range of ceramic materials have been used in preclinical orthopedic antimicrobial applications including calcium sulfate, , hydroxyapatite, , β-tricalcium phosphate, zirconium nitride, borate bioactive glass, and calcium phosphate , (Table ). Several ceramics are intrinsically antimicrobial, such as calcium phosphate and bioactive glass; , however, for orthopedic implant infection applications, ceramics often incorporate antibiotics.…”
Section: Advantages and Limitations Of Antimicrobial Biomaterials Use...mentioning
confidence: 99%
“…Additionally, 10.3389/fmicb.2022.1029261 Frontiers in Microbiology 03 frontiersin.org β-TCP/CS has an osteoconductive effect and provides bone scaffolding (Urban et al, 2007;Fernandez de Grado et al, 2018;Zhao et al, 2020). Here, we analyzed the release characteristics of DAP and compared the efficiency of DAP with that of VAN in preventing and killing MRSA biofilms, concerning a previously established in vitro biofilm model (Jiang et al, 2021). Through these experiments, we provide a theoretical basis for the local administration of DAP for the treatment of IAIs in clinical settings.…”
Section: Introductionmentioning
confidence: 99%
“…The most challenging complications following joint replacement and fracture fixation procedures are implant-associated infections (IAIs), including periprosthetic joint infections (PJIs) and fracture-related infections (FRIs) ( Trampuz and Widmer, 2006 ; Horton et al, 2021 ). According to recent studies, the cumulative incidence after primary hip or knee arthroplasty is 0.5% in the first year and 1.4% within 10 years, whereas the incidence of FRI ranges from 0.4 to 16.1%, with an average of 5%, and up to 30% following open fractures ( Beam and Osmon, 2018 ; Foster et al, 2020 ; Jiang et al, 2021 ). Despite advances in surgical techniques, IAIs still pose great challenges to orthopedic surgeons, owing to their high risk of disability, mortality, and infection recurrence.…”
Section: Introductionmentioning
confidence: 99%