Antibiotic pigment from desert soil actinomycetes; biological activity, purification and chemical screening

Selvameenal, L; Radhakrishnan, M.; Balagurunathan, Ramasamy

doi:10.4103/0250-474x.58174

Cited by 80 publications

(22 citation statements)

References 13 publications

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“…Ethyl acetate fractions were concentrated under vacuo with a rotary evaporator. The dried crude extract was weighed and reconstituted with the appropriate solvent for chemical analysis and bioassay screening [49, 50].…”

Section: Methodsmentioning

confidence: 99%

Bioassay- and metabolomics-guided screening of bioactive soil actinomycetes from the ancient city of Ihnasia, Egypt

et al. 2019

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Literature surveys, taxonomical differences, and bioassay results have been utilized in the discovery of new natural products to aid in Actinomycetes isolate-selection. However, no or less investigation have been done on establishing the differences in metabolomic profiles of the isolated microorganisms. The study aims to utilise bioassay- and metabolomics-guided tools that included dereplication study and multivariate analysis of the NMR and mass spectral data of microbial extracts to assist the selection of isolates for scaling-up the production of antimicrobial natural products. A total of 58 actinomycetes were isolated from different soil samples collected from Ihnasia City, Egypt and screened for their antimicrobial activities against indicator strains that included Bacillus subtilis, Escherichia coli, methicillin-resistant Staphylococcus aureus and Candida albicans. A number of 25 isolates were found to be active against B. subtilis and/or to at least one of the tested indicator strains. Principal component analyses showed chemical uniqueness for four outlying bioactive actinomycetes extracts. In addition, Orthogonal Projections to Latent Structures Discriminant Analysis (OPLS-DA) and dereplication study led us to further select two outlying anti-MRSA active isolates MS.REE.13 and 22 for scale-up work. MS.REE.13 and 22 exhibited zones of inhibition at 19 and 13 mm against MRSA, respectively. A metabolomics-guided approach provided the steer to target the bioactive metabolites (P<0.01) present in a crude extract or fraction even at nanogram levels but it was a challenge that such low-yielding bioactive natural products would be feasible to isolate. Validated to occur only on the active side of OPLS-DA loadings plot, the isolated compounds exhibited medium to weak antibiotic activity with MIC values between 250 and 800 μM. Two new compounds, P_24306 (C10H13N2) and N_12799 (C18H32O3) with MICs of 795 and 432 μM, were afforded from the scale-up of MS.REE. 13 and 22, respectively.

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Section: Methodsmentioning

confidence: 99%

Bioassay- and metabolomics-guided screening of bioactive soil actinomycetes from the ancient city of Ihnasia, Egypt

et al. 2019

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“…Wells were bored by sterilized 1000 μl micro tip, and 100 μl of each crude extract was poured into wells. Plates were incubated at 37°C for 24 h.[26]…”

Section: Methodsmentioning

confidence: 99%

Antibacterial activity of actinomycetes isolated from different soil samples of Sheopur (A city of central India)

Chaudhary¹,

Yadav²,

Shrivastava³

et al. 2013

J Adv Pharm Technol Res

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The main objective of the present study was isolation, purification, and characterization of actinomycetes from soil samples, having antimicrobial activity against 12 selected pathogenic strains. Soils samples were taken from different niche habitats of Sheopur district, Madhya Pradesh, India. These samples were serially diluted and plated on actinomycete isolation agar media. Potential colonies were screened, purified, and stored in glycerol stock. Isolates were morphologically and biochemically characterized. These isolates were subjected to extraction for production of the antibacterial compound. Antibacterial activity and Minimum Inhibitory Concentration (MIC) of the purified extract of isolates were evaluated. Totally 31 actinomycete isolates were tested for antagonistic activity against 12 pathogenic microorganisms. Isolates AS14, AS27, and AS28 were highly active, while AS1 showed less activity against the pathogenic microorganisms. Isolate AS7 exhibited the highest antagonistic activity against Bacillus cereus (24 mm) and AS16 showed the highest activity against Enterococcus faecalis (21 mm). MIC was also determined for actinomycete isolates against all the tested microorganisms. MIC of actinomycete isolates was found to be 2.5 mg/ml against Shigella dysenteriae, Vancomycin-resistant enterococci, and Klebsiella pneumoniae, and was 1.25 mg/ml for Staphylococcus saprophyticus, Streptococcus pyogenes, Staphylococcus epidermidis, Methicillin-resistant Staphylococcus, Bacillus cereus, Staphylococcus xylosus, Methicillin-resistant Staphylococcus aureus, Enterococcus faecalis, and Staphylococcus aureus. All actinomycetes isolates showed antibacterial activity against S. aureus, while they showed less activity against S. dysenteriae. These isolates had antibacterial activity and could be used in the development of new antibiotics for pharmaceutical or agricultural purposes.

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“…The changing pattern of diseases and the emergence of resistant bacterial strains to currently used antibiotics continuously put demand on the drug discovery scientists to search for novel antibiotics such as bacterial pigments ( 20 ). In an attempt to isolate the potential pigment producing bacteria, sea water samples were collected from intertidal zones of Visakhapatnam coastal region, India.…”

Section: Discussionmentioning

confidence: 99%

Antibacterial and anti-inflammatory activities of marine Brevibacterium sp.

et al. 2017

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The marine environment covers three quarters of the surface of the planet and is estimated to be home to more than 80% of life but yet it remains largely unexplored. It harbours a number of macro and microorganisms that have developed unique metabolic abilities to ensure their survival in diverse and hostile habitats, resulting in the biosynthesis of an array of secondary metabolites with specific activities. In this study, pigment forming bacterial strains were isolated from the sea surface inter tidal zones at different sampling sites along the Visakhapatnam coastal region. The bacterial isolates showed various types of colour pigments like pink, yellow, orange, and brown. Out of 26 pigmented isolates obtained, the bacterial isolate with bright yellow pigmentation was selected for further study. This strain was identified as Brevibacterium sp by using morphological, physiological, biochemical and 16s rRNA sequencing methods. The pigment was extracted in methanol solvent and antibacterial activity of the pigments extracted from the bacteria was determined and it was found active against pathogenic bacteria. The pigment extract was tested In vivo for anti-inflammatory activity and was effective.

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Antibiotic pigment from desert soil actinomycetes; biological activity, purification and chemical screening

Cited by 80 publications

References 13 publications

Bioassay- and metabolomics-guided screening of bioactive soil actinomycetes from the ancient city of Ihnasia, Egypt

Bioassay- and metabolomics-guided screening of bioactive soil actinomycetes from the ancient city of Ihnasia, Egypt

Antibacterial activity of actinomycetes isolated from different soil samples of Sheopur (A city of central India)

Antibacterial and anti-inflammatory activities of marine Brevibacterium sp.

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