Antibiotic-Potentiating Activity of the Schinus terebinthifolius Raddi Essential Oil against MDR Bacterial Strains

Abstract

Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus are the primary bacteria that cause clinical infections, such as urinary and intestinal infections, pneumonia, endocarditis, and sepsis. Bacterial resistance is an innate natural occurrence in microorganisms, resulting from mutations or the lateral exchange of genetic material. This serves as evidence for the association between drug consumption and pathogen resistance. Evidence has demonstrated that the association between conventional antibiotics and natural products is a promising pharmacological strategy to overcome resistance mechanisms. Considering the large body of research demonstrating the significant antimicrobial activities of Schinus terebinthifolius Raddi, the present study aimed to evaluate the chemical composition and antibiotic-enhancing effects of Schinus terebinthifolius Raddi essential oil (STEO) against the standard and multidrug-resistant strains of Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus. The STEO was extracted by hydrodistillation using a Clevenger-type vacuum rotary evaporator. The Minimum Inhibitory Concentration (MIC) of the STEO was assessed by the microdilution method to evaluate the antibacterial activity. The antibiotic-enhancing activity of the essential oil was assessed by determining the MIC of antibiotics in the presence of a sub-inhibitory concentration (MIC/8) of the natural product. The GC-MS analysis revealed alpha-pinene (24.3%), gamma-muurolene (16.6%), and myrcene (13.7%) as major constituents of the STEO. The STEO potentiated the enhanced antibacterial activity of norfloxacin and gentamicin against all the strains and increased the action of penicillin against the Gram-negative strains. Therefore, it is concluded that although the STEO does not exhibit clinically effective antibacterial activity, its association with conventional antibiotics results in enhanced antibiotic activity.

Chemical Composition and Antibacterial and Antiulcerative Colitis Activities of Essential Oil from Pruni Semen

“…42 E. coli, P. aeruginosa, S. aureus, and S. epidermidis are the primary bacteria causing intestinal infections. 44 E. coli, the Gram-negative bacteria, exacerbated intestinal inflammation by invading intestinal epithelial cells. 45 S. aureus and S. epidermidis, the Gram-positive bacteria, presented significant effects on the number of goblet cells, crypt depth, and villi height.…”

Antioxidant, Antimicrobial, and Anti-Inflammatory Effects of Liriodendron chinense Leaves

“…Finally, the absorbance was measured at 700 nm after adequately shaking. 20 In brief, normal saline was used to dissolve and dilute LCLE samples, together with the conventional antibacterial agent chloramphenicol. Each embraced standard solutions (10 6 CFU/mL) of each of the microorganisms, with varying final concentrations of either the common antimicrobial antibiotic chloramphenicol (1000−15.625 μg/mL) or LCLE (50− 1.5625 mg/mL).…”

Chemical Composition, Antibacterial and Inhibitory Activity of the Efflux Pump of Essential Oils from Croton piauhiensis Müll.

“…In view of these facts, the scientific community has intensified studies in the search for more new effective drugs with a lower level of toxicity. Alternative ways as in vitro methods in antimicrobial tests to evaluate the potential of extracts, essential oils and isolated compounds have been widely discussed by science, revealing potential antimicrobial agents, or even potentiating the activity of antibiotics used in clinical practice [17][18][19][20]. Thus, the combination of products may provide antimicrobial efficacy at doses that were previously ineffective due to the antimicrobial resistance presented by some strains [20][21][22].…”