Antibiotic resistance and class 1 integron gene dynamics along effluent, reclaimed wastewater irrigated soil, crop continua: elucidating potential risks and ecological constraints

Marano, Roberto B. M.; Zolti, Avihai; Jurkevitch, Édouard; Cytryn, Eddie

doi:10.1016/j.watres.2019.114906

Cited by 62 publications

(39 citation statements)

References 48 publications

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“…Wastewater-irrigated soil in lysimeters had higher relative and absolute abundance of intl1 genes compared to freshwater-irrigated lysimeters. However, almost all ARGs were below detection limits in all tested soils, even after irrigation with treated wastewater [ 42 ]. A third study in Israel compared soils irrigated with greywater treated by constructed wetlands to soils irrigated with freshwater, with no difference in the abundance of tetracycline-resistant bacteria between the two types of soils [ 43 ].…”

Section: Resultsmentioning

confidence: 99%

Does Irrigation with Treated and Untreated Wastewater Increase Antimicrobial Resistance in Soil and Water: A Systematic Review

Slobodiuk

Niven

Arthur

et al. 2021

IJERPH

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Population growth and water scarcity necessitate alternative agriculture practices, such as reusing wastewater for irrigation. Domestic wastewater has been used for irrigation for centuries in many historically low-income and arid countries and is becoming more widely used by high-income countries to augment water resources in an increasingly dry climate. Wastewater treatment processes are not fully effective in removing all contaminants, such as antimicrobial resistant bacteria (ARB) and antimicrobial resistance genes (ARGs). Literature reviews on the impact of wastewater irrigation on antimicrobial resistance (AMR) in the environment have been inconclusive and mostly focused on treated wastewater. We conducted the first systematic review to assess the impact of irrigation with both treated or untreated domestic wastewater on ARB and ARGs in soil and adjacent water bodies. We screened titles/abstracts of 3002 articles, out of which 41 were screened in full text and 26 were included in this review. Of these, thirteen investigated irrigation with untreated wastewater, and nine found a positive association with ARB/ARGs in soil. Out of thirteen studies focused on treated wastewater, six found a positive association with ARB/ARGs while six found mixed/negative associations. Our findings demonstrate that irrigation with untreated wastewater increases AMR in soil and call for precautionary action by field workers, their families, and consumers when untreated wastewater is used to irrigate crops. The effect of irrigation with treated wastewater was more variable among the studies included in our review, highlighting the need to better understand to what extent AMR is disseminated through this practice. Future research should assess factors that modify the effect of wastewater irrigation on AMR in soil, such as the degree and type of wastewater treatment, and the duration and intensity of irrigation, to inform guidelines on the reuse of wastewater for irrigation.

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Section: Resultsmentioning

confidence: 99%

Does Irrigation with Treated and Untreated Wastewater Increase Antimicrobial Resistance in Soil and Water: A Systematic Review

Slobodiuk

Niven

Arthur

et al. 2021

IJERPH

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“…In the absence of sufficient data and adequate methods to support a robust risk assessment focused on antibiotic resistance, it is valuable to identify approaches that can be used to establish a baseline level of risk in the interim. (Garner et al, 2018(Garner et al, , 2019Harb et al, 2019;Marano et al, 2019;Kampouris et al, 2021), which has been proposed as a monitoring target for antibiotic resistance given the ability of this integron to carry multiple ARGs and its propensity for horizontal gene transfer (Berendonk et al, 2015;Gillings et al, 2015). Others used metagenomic sequencing to identify ARGs associated mobile genetic elements, such as plasmids (Garner et al, 2018) In addition to the need to ensure that reported ARB and ARG data in recycled water can inform efforts at risk assessment, there are several key research gaps that remain in how to apply traditional QMRA approaches to antibiotic resistant pathogens.…”

Section: Integration Of Monitoring Results Into Risk Assessment Frameworkmentioning

confidence: 99%

Towards risk assessment for antibiotic resistant pathogens in recycled water: a systematic review and summary of research needs

Garner

Organiscak

Dieter

et al. 2021

Environmental Microbiology

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Risk assessment is critical for identifying target concentrations of antibiotic resistant pathogens necessary for mitigating potential harmful exposures associated with water reuse. However, there is currently limited available data characterizing the concentrations of antibiotic resistant bacteria (ARB) and antibiotic resistance genes (ARGs) in recycled water to support robust efforts at risk assessment. The objective of this systematic review was to identify and synthesize the existing literature documenting the presence and abundance of ARB and ARGs in recycled water. In addition, this review identifies best practices and explores monitoring targets for studying ARB and ARGs in recycled water to guide future work and identifies key research needs aimed at better supporting quantitative microbial risk assessment focused on recycled water and antibiotic resistance. Future efforts to collect data about ARB and ARG prevalence in recycled water should report concentration data per unit volume. Sample metadata should also be provided, including a description of treatment approach, a description of planned water uses (e.g., potable, irrigation), methods for conveyance to the point of use, and available physicochemical water quality data. Additional research is needed aimed at identifying recommended ARB and ARG monitoring targets and for developing approaches to incorporate metagenomic data into risk assessment.

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“…Studies evaluating the prevalence of AMR in WWTP effluent indicate that wastewater treatment processes, including some secondary and tertiary treatment and disinfection processes, may not fully eliminate AMR bacteria and ARGs and that surviving bacteria retain their resistance properties [41,72,97,113,115,[122][123][124][125][126][127][128][129]. Hiller et al [72] reviewed the available literature on the levels of AMR bacteria and ARGs present following treatment with common WWTP processes, including disinfection with UV, ozone, and chlorine, to assess their efficacy.…”

Section: Treated Wastewatermentioning

confidence: 99%

“…Hiller et al [72] concluded "these findings confirm that discharge from WWTPs can result in significant contributions of AMR to the aquatic environment." Treatment processes commonly used at WWTPs are not typically calibrated to target removal of AMR bacteria or ARGs, with concerns about persistence and transformation of both cell-associated ARGs and extracellular DNA coding for antibiotic resistance [127,128]. The effectiveness of some disinfectants, including chlorine and ozone, are impacted by dose, contact time, temperature, and water quality variables (e.g., pH, turbidity, presence of ammonia and oxidant demand) [130,131].…”

Section: Treated Wastewatermentioning

confidence: 99%

Antibiotic Resistance in Recreational Waters: State of the Science

Nappier

Liguori

Ichida

et al. 2020

IJERPH

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Ambient recreational waters can act as both recipients and natural reservoirs for antimicrobial resistant (AMR) bacteria and antimicrobial resistant genes (ARGs), where they may persist and replicate. Contact with AMR bacteria and ARGs potentially puts recreators at risk, which can thus decrease their ability to fight infections. A variety of point and nonpoint sources, including contaminated wastewater effluents, runoff from animal feeding operations, and sewer overflow events, can contribute to environmental loading of AMR bacteria and ARGs. The overall goal of this article is to provide the state of the science related to recreational exposure and AMR, which has been an area of increasing interest. Specific objectives of the review include (1) a description of potential sources of antibiotics, AMR bacteria, and ARGs in recreational waters, as documented in the available literature; (2) a discussion of what is known about human recreational exposures to AMR bacteria and ARGs, using findings from health studies and exposure assessments; and (3) identification of knowledge gaps and future research needs. To better understand the dynamics related to AMR and associated recreational water risks, future research should focus on source contribution, fate and transport—across treatment and in the environment; human health risk assessment; and standardized methods.

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Antibiotic resistance and class 1 integron gene dynamics along effluent, reclaimed wastewater irrigated soil, crop continua: elucidating potential risks and ecological constraints

Cited by 62 publications

References 48 publications

Does Irrigation with Treated and Untreated Wastewater Increase Antimicrobial Resistance in Soil and Water: A Systematic Review

Does Irrigation with Treated and Untreated Wastewater Increase Antimicrobial Resistance in Soil and Water: A Systematic Review

Towards risk assessment for antibiotic resistant pathogens in recycled water: a systematic review and summary of research needs

Antibiotic Resistance in Recreational Waters: State of the Science

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