Antibiotic resistance and ESBL production in Escherichia coli from various sources in Aba metropolis, Nigeria

Ajuga, Martha Uzoaru; Otokunefor, Kome; Agbagwa, O. E.

doi:10.1186/s42269-021-00628-5

Cited by 8 publications

(8 citation statements)

References 44 publications

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“…The global incidence of ESBL-producing bacteria is on a rising trend ranging from 33 to 91% in reported cases [40][41][42][43]. Our study identified the presence of ESBL-producing MDR Aeromonas strains in the aquatic environment with fisheries operations posing a substantial public health risk.…”

Section: Discussionmentioning

confidence: 74%

Prevalence of Extended-Spectrum β-Lactamases (ESBLs) Producing Aeromonas spp. Isolated from Lamellidens marginalis (Lamark, 1819) of Sewage-Fed Wetland: A Phenotypic and Genotypic Approach

Mohanty,

Das,

Kumari

et al. 2024

Microorganisms

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The global rise of zoonotic bacteria resistant to multiple antimicrobial classes and the growing occurrence of infections caused by Aeromonas spp. resistant to β-lactam antibiotics pose a severe threat to animal and human health. However, the contribution of natural environments, particularly aquatic ecosystems, as ideal settings for the development and spread of antimicrobial resistance (AMR) is a key concern. Investigating the phenotypic antibiotic resistance and detection of β-lactamase producing Aeromonas spp. in Lamellidens marginalis, which inhabit all freshwater ecosystems of the Indian subcontinent, is essential for implications in monitoring food safety and drug resistance. In the present investigation, 92 isolates of Aeromonas spp. were recovered from 105 bivalves and screened for their antimicrobial resistance patterns. In vitro antibiotic resistance profiling showed a higher Multiple Antibiotic Resistance (MAR) index of 0.8 with the highest resistance against ampicillin/sulbactam (82%), while 58, 44, 39 and 38% of the isolates were resistant to cephalothin, erythromycin, cefoxitin and imipenem, respectively. PCR results revealed that these isolates carried the blaTEM gene (94%), which was followed by the blaCTX-M gene (51%) and the blaSHV gene (45%). A combination of blaSHV, blaCTX-M, and blaTEM genes was found in 17% of the isolates, indicating the presence of all three resistance genes. This is the first investigation which highlights the importance of multidrug-resistant Aeromonas spp. in L. marginalis. The identification of extended-spectrum-β-lactamases (ESBLs) genes demand the necessity of continuous surveillance and systematic monitoring, considering its potential health risks for both animals and human beings.

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Section: Discussionmentioning

confidence: 74%

Prevalence of Extended-Spectrum β-Lactamases (ESBLs) Producing Aeromonas spp. Isolated from Lamellidens marginalis (Lamark, 1819) of Sewage-Fed Wetland: A Phenotypic and Genotypic Approach

Mohanty,

Das,

Kumari

et al. 2024

Microorganisms

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“…Characteristic colonies were then purified and stored. Following this, DNA was obtained from pure cultures using the boiling method (Ajuga et al, 2021). In brief, pure cultures were emulsified in 100 µl volume of sterile DNase free distilled water.…”

Section: A Isolation and Identification Of Test Strainsmentioning

confidence: 99%

Molecular detection of virulence and resistance markers in Escherichia coli and Staphylococcus aureus isolated from Nigerian currency

Otokunefor

Onyemelukwe

Agbake

et al. 2022

JLBSR

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Potentially pathogenic bacteria are routinely linked with different currencies worldwide. Not much focus has been on the actual pathogenic potential of these bacteria. This is essential as the risk would depend on whether the bacteria associated with the currency is a commensal or pathogen. This study was therefore aimed at exploring the virulence potential of Escherichia coli and Staphylococcus aureus isolated from specific Nigerian currency denominations. Presumptive E. coli and S. aureus isolates were obtained from the samples and their identities confirmed genotypically. A total of seven virulence and resistance genes were tested for (aggR, eae, ast, van, cat, pvl, icaA). Three of these were E. coli virulence genes (aggR, eae, ast), two S. aureus virulence (pvl, icaA) and two S. aureus resistance (van, cat). Sixty presumptive E. coli and S. aureus (30 each) were isolated and purified. Of these, 9 (30%) were confirmed as E. coli following genotypic identification and 7 (23%) as S. aureus. An assessment of the virulence potential of E. coli showed 100% detection of the ast gene, 55.6% detection of aggR and 44.4% detection of eae. For S. aureus, a much lower frequency of test genes was found with rates of 41.7%, 16.7%, 16.7% and 8.3% for pvl, icaA, van and cat respectively. This study therefore reports a low frequency of S. aureus virulence genes. The E. coli strains however, rather than being innocuous carry virulence factors. Furthermore, some of these have a known association with mobile genetic elements and hence a capacity to transform harmless commensal strains to pathogens.

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“…Antimicrobial susceptibility of the isolates was carried out using the modified Kirby-Bauer Disk Diffusion method [13]. Mueller Hinton (MH) Agar (Oxoid, UK) was prepared according to the manufacturer's instructions and 10ml transferred into 90 mm diameter sterile Petri dishes making a depth of 4 mm.…”

Section: Antimicrobial Susceptibility Testmentioning

confidence: 99%

Prevalence of Extended-spectrum Beta-lactamase Mediated Urinary Tract Infections in a Tertiary Hospital in Enugu, South East, Nigeria

Ugwu

Ogbuabor

Anyamene

2022

SAJRM

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Extended-Spectrum Beta-Lactamase (ESBL) are a class of plasmid-mediated diverse, complex and rapidly evolving enzymes which hydrolyzes penicillins, monobactams and broad-spectrum cephalosporins. There is increasing prevalence of ESBL-producing bacteria globally with higher prevalence reported in developing countries. The present study was designed to determine the prevalence and antimicrobial sensitivity pattern of ESBL producing bacteria isolates in patients with Urinary Tract Infection (UTI) in Enugu. Urine samples were collected from a total of 284 subjects with self-reported UTI In the Enugu State University of Science and Technology Teaching Hospital, Enugu State, Nigeria between the periods of April to May,2019.Samples were analyzed for the presence of UTI and antimicrobial resistance pattern of ESBL-producing bacteria using standard methods. Data was analyzed by descriptive statistics using SPSS version 23 (IBM Corp Armok, NY). A total of 145 strains of Staphylococcus aureus (n= 19). Escherichia coli (n=52), Klebsiella pneumonia (n=45), Pseudomonas aeruginosa (n =12), Enterococcus faecalis (n=9) and Proteus mirabilis (n=8), were isolated from 132 subjects giving a UTI prevalence rate of 34.6%. Forty-nine (49) ESBL-producing strains comprising Staphylococcus aureus (n=5), Escherichia coli (n=15), Klebsiella pneumonia (n=16), Pseudomonas aeruginosa (n=13), Enterococcus faecalis (n=8) and Proteus mirabilis (n=7) were implicated in UTI accounting for 46.8%ESBL-producing bacteria mediated UTI. There is a high prevalence of ESBL-producing bacteria induced UTI. This portends a great danger to the management of bacterial infections.

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Antibiotic resistance and ESBL production in Escherichia coli from various sources in Aba metropolis, Nigeria

Cited by 8 publications

References 44 publications

Prevalence of Extended-Spectrum β-Lactamases (ESBLs) Producing Aeromonas spp. Isolated from Lamellidens marginalis (Lamark, 1819) of Sewage-Fed Wetland: A Phenotypic and Genotypic Approach

Prevalence of Extended-Spectrum β-Lactamases (ESBLs) Producing Aeromonas spp. Isolated from Lamellidens marginalis (Lamark, 1819) of Sewage-Fed Wetland: A Phenotypic and Genotypic Approach

Molecular detection of virulence and resistance markers in Escherichia coli and Staphylococcus aureus isolated from Nigerian currency

Prevalence of Extended-spectrum Beta-lactamase Mediated Urinary Tract Infections in a Tertiary Hospital in Enugu, South East, Nigeria

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