Antibiotic resistance and microbial composition along the manufacturing process of Mozzarella di Bufala Campana

Devirgiliis, Chiara; Caravelli, Antonella; Coppola, Doriana; Barile, Simona N.; Perozzi, Giuditta

doi:10.1016/j.ijfoodmicro.2008.09.021

Cited by 56 publications

(58 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Diverse antibiotic resistance incidences were observed in different Chinese fermented foods Antibiotics had been spread in the environment when used as growth promoters in livestock years ago, leading to the selection of Table 1 List of primers and PCR conditions used in this study. (Devirgiliis, Caravelli, Coppola, Barile, & Perozzi, 2008). These bacteria may reside in or on fruits, vegetables and animal feeds (Pernezny, K udela, Koko sková, & Hládká, 1995;Umesha, 2006) and end up in the fermented foods (Fu, Xi, & Liu, 2008).…”

Section: Resultsmentioning

confidence: 99%

“…Since samples were collected from different cities and processed by different factories, materials could be harvested from various regions and processed in different conditions of antibiotic pollution (Devirgiliis et al, 2008), resulting in the diversity of ARIs. Furthermore, the reuse of some starter culture, such as some pickle juice, could make the spread of AR inescapable.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Assessment of antibiotic resistance of lactic acid bacteria in Chinese fermented foods

Lu¹,

Hu²,

Wang³

2011

Food Control

View full text Add to dashboard Cite

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Assessment of antibiotic resistance of lactic acid bacteria in Chinese fermented foods

Lu¹,

Hu²,

Wang³

2011

Food Control

View full text Add to dashboard Cite

“…Identification of 21 L. paracasei isolates displaying phenotypic resistance to tetracycline (MIC ϭ 32 mg/liter) from an Italian cheese (Mozzarella di Bufala Campana DOP) was previously (9). The isolates were found in raw milk and natural whey starter cultures from one of the three cheese factories analyzed.…”

Section: Paracasei Harbors the Tet(m) Gene Associated With Tn916mentioning

confidence: 99%

“…In the present study, we have characterized at the molecular level a group of tetracycline-resistant L. paracasei isolates, previously identified in raw milk and natural whey starter cultures employed in the manufacture of the Italian traditional cheese Mozzarella di Bufala Campana (9). We provide evidence that in these isolates, tetracycline resistance is due to the presence of the conjugative transposon Tn916, carrying the tet(M) gene and capable of horizontal, interspecies transfer to the opportunistic pathogen Enterococcus faecalis via a circular intermediate containing a novel coupling sequence that confers a lowfrequency-conjugation phenotype.…”

mentioning

confidence: 99%

Characterization of the Tn 916 Conjugative Transposon in a Food-Borne Strain of Lactobacillus paracasei

Devirgiliis

Coppola

Barile

et al. 2009

Appl Environ Microbiol

Self Cite

View full text Add to dashboard Cite

Food-borne antibiotic-resistant lactic acid bacteria have received growing attention in the past few years. We have recently identified tetracycline-resistant Lactobacillus paracasei in samples of milk and natural whey starter cultures employed in the manufacturing process of a typical Italian fermented dairy product, Mozzarella di Bufala Campana. In the present study, we have characterized at the molecular level the genetic context of tetracycline resistance determinants in these natural strains, which we have identified as tet(M). This gene was present in 21 independent isolates, whose fingerprinting profiles were distributed into eight different repetitive extragenic palindromic groups by cluster analysis. We provide evidence that the gene is associated with the broad-host, conjugative transposon Tn916, which had never before been described to occur in L. paracasei. PCR analysis of four independent isolates by use of specifically designed primer pairs detected the presence of a circular intermediate form of the transposon, carrying a coupling sequence (GGCAAA) located between the two termini of Tn916. This novel coupling sequence conferred low conjugation frequency in mating experiments with the recipient strain JH2-2 of Enterococcus faecalis.

show abstract

“…Nowadays, PCR is the most common method used for studying the carriage of AR genes in food systems, and several reports have described the use of different molecular approaches based on PCR techniques, including quantitative Real Time PCR, for detecting resistance determinants and for monitoring antimicrobial-resistant bacteria in foods (Devirgiliis, Caravelli, Coppola, Barile, & Perozzi, 2008;Garofalo et al, 2007;Manuzon et al, 2007). However, if every possible resistance gene had to be independently assessed, the amount of work required would be large and time consuming.…”

Section: Introductionmentioning

confidence: 99%

Development and validation of a multiplex PCR-based DNA microarray hybridisation method for detecting bacterial antibiotic resistance genes in cheese

Delgado

Fracchetti

Mayo

et al. 2011

International Dairy Journal

View full text Add to dashboard Cite

a b s t r a c tThe aim of this study was to develop a method for detecting antibiotic resistance (AR) genes in cheese based on a combination of multiplex PCR and a DNA microarray hybridisation system. Twenty oligonucleotide probes were designed targeting 10 common AR genes, namely aac(6 0 )-Ie-aph(2 00 )-Ia, aadE, aphA-3, ermB, tet(L), tet(M), tet(O), tet(S), vanA and vanB. Specificity of the probes was tested by hybridising against DNA from Enterococcus strains harbouring known AR genes. DNA was labelled through two multiplex PCR reactions with fluorescent nucleotides and specific primers flanking the probe sequences. Sensitivity of the microarray was assessed by contamination of a cheese with an Enterococcus faecium strain carrying vanA gene. Two tetracycline resistance genes, tet(M) and tet(S), proved to be present in a series of retail cheeses, while genes aadE, aphA3, ermB, tet(L) and tet(O) were occasionally detected. This method is envisioned as a valuable tool for identification of AR genes in foods.

show abstract

Antibiotic resistance and microbial composition along the manufacturing process of Mozzarella di Bufala Campana

Cited by 56 publications

References 28 publications

Assessment of antibiotic resistance of lactic acid bacteria in Chinese fermented foods

Assessment of antibiotic resistance of lactic acid bacteria in Chinese fermented foods

Characterization of the Tn 916 Conjugative Transposon in a Food-Borne Strain of Lactobacillus paracasei

Development and validation of a multiplex PCR-based DNA microarray hybridisation method for detecting bacterial antibiotic resistance genes in cheese

Contact Info

Product

Resources

About