Antibiotic resistance and OXA-type carbapenemases-encoding genes in airborne Acinetobacter baumannii isolated from burn wards

Gao, Jing; Zhao, Xiaonan; Bao, Ying; Ma, Rujiang; Zhou, Yufa; Li, Xinxian; Chai, Tongjie; Cai, Yanbin

doi:10.1016/j.burns.2013.06.003

Cited by 39 publications

(31 citation statements)

References 17 publications

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“…The presence of blaOXA-23-like genes was observed in all the strains. In 2013, OXA-23-like encoded genes were detected in 94% of isolated A. baumannii in China (36). Additionally, the results of a study conducted in Iran in 2012 indicated that blaOXA-23-like genes were detected in all the strains (37), which is consistent with our results.…”

Section: Discussionsupporting

confidence: 82%

Detection of ISAba1 in Acinetobacter baumannii Strains Carrying OXA Genes Isolated From Iranian Burns Patients

Mohammadi

Goudarzi

Hashemi

et al. 2016

Arch Pediatr Infect Dis

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Background: The presence of carbapenemase-producing Acinetobacter baumannii has become a growing concern in patients who are hospitalized in burns centers. Objectives: The aims of this study were to determine the antimicrobial susceptibility patterns and prevalence of blaOXA carbapenemases, as well as to detect the presence of ISAba1, in A. baumannii strains carrying OXA genes obtained from burns patients at Shahid Motahari hospital, Tehran, Iran. Methods: From August 2013 to March 2014, 100 clinical A. baumannii isolates were collected from patients who were admitted to the burns ward at Shahid Motahari hospital. Antimicrobial susceptibility was determined using a disc diffusion test. PCR, sequencing, and multiplex PCR were used for the detection of blaOXA-23-like, blaOXA-51-like, blaOXA-24-like, and blaOXA-58-like genes, which were then sequenced. The ISAba1 gene was detected, and PCR was performed to detect the presence of ISAba1/blaOXA-51-like and ISAba1/blaOXA-23-like genes. Results:The results showed that 93% of the strains were multi-drug resistant, while 82% of them were extensively drug resistant. Additionally, all the strains carried blaOXA-23-like and blaOXA-51-like genes, while 74% and 0% of the strains harbored blaOXA-24-like and blaOXA-58 genes, respectively. ISAba1 was detected in all the strains except for one. The co-existence of ISAba1/blaOXA-51-like genes and ISAba1/blaOXA-23-like genes was detected in 65% and 80% of strains, respectively. Conclusions:The results of this study indicate that the emergence of OXA-type carbapenemases in A. baumannii causing nosocomial infections in burns patients could be of importance for hospital infection control systems in Iran.

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Section: Discussionsupporting

confidence: 82%

Detection of ISAba1 in Acinetobacter baumannii Strains Carrying OXA Genes Isolated From Iranian Burns Patients

Mohammadi

Goudarzi

Hashemi

et al. 2016

Arch Pediatr Infect Dis

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“…However, the lack of A. baumannii found differs from the small number of other studies where A. baumannii has been found more frequently in the air. 2–4 Published studies on this topic are few; use differing air sampling techniques, and note minimal patient level data making it difficult to draw conclusions. 2–4 …”

Section: Discussionmentioning

confidence: 99%

“…3 A 2011 study performed in China, using a similar air impactor to what we used in this study, found A. baumannii in 16 air samples. 4 Air was sampled for just 10 minutes using the 6 stage Anderson impactor. However, information regarding number of air samples taken, proximity of sampler to patients or patient factors was not provided.…”

Section: Discussionmentioning

confidence: 99%

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Infrequent Air Contamination WithAcinetobacter baumanniiof Air Surrounding Known Colonized or Infected Patients

Rock

Harris

Johnson

et al. 2015

Infect. Control Hosp. Epidemiol.

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“…OXA types have a weak hydrolysis property but they are usually associated with genetic elements such as insertion sequences to increase the expression of carbapenemases and also mobilize them (7,9). genes, such as bla OXA-23-like genes, bla OXA-51-like genes, and bla OXA-58-like genes, it increases their expression to a level that confers resistance to carbapenem (5,10,11). Therefore, determining the prevalence of IS as well as the OAXs genes in A. baumannii in different hospitals and medical centers is very important.…”

Section: Introductionmentioning

confidence: 99%

Identification and Isolation of Insertion Sequences, in Carbapenem Resistant Clinical Isolates of Acinetobacter baumannii from Tehran Hospitals

Owrang

Fallah²,

Irani

et al. 2017

Jundishapur J Microbiol

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Background: Identification of real agents inducing antibiotic resistance in Acinetobacter baumannii can help us control and prevent the infections and reduce the mortality rates caused by this microorganism. Objectives: The purpose of this study was to identify the insertion elements that can play important roles in antibiotic resistance in A. baumannii.Methods: A total of 105 A. baumannii isolates from clinical samples were tested for their susceptibility to different antibiotics using disc diffusion test (DDT). Then, the isolates were evaluated for the presence of blaOXA genes along with IS elements by polymerase chain reaction (PCR). Results: PCR analysis showed that ISAba1 was present in all the isolates while 92.36% of the isolates were positive for ISAba2. All the strains of A. baumannii possessed a blaOXA-51-like gene but blaOXA-58-like gene was absent in all of them. About 64.76% of the isolates were positive for blaOXA-24-like gene and 98.09% were positive for blaOXA-23-like gene. All of the isolates were pandrug resistant A. baumannii and the lowest resistance rates were observed toward minocycline and amikacin (93.33% in both) and trimethoprim/sulfamethoxazole (92.38%). Conclusions:The high prevalence rates of ISAba1 and ISAba2 among A. baumannii isolates can explain the rapid dissemination of resistance genes. Thus, finding the accessory genes such as IS elements, which can affect antibiotic resistance, should be more considered.

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Antibiotic resistance and OXA-type carbapenemases-encoding genes in airborne Acinetobacter baumannii isolated from burn wards

Cited by 39 publications

References 17 publications

Detection of ISAba1 in Acinetobacter baumannii Strains Carrying OXA Genes Isolated From Iranian Burns Patients

Detection of ISAba1 in Acinetobacter baumannii Strains Carrying OXA Genes Isolated From Iranian Burns Patients

Infrequent Air Contamination WithAcinetobacter baumanniiof Air Surrounding Known Colonized or Infected Patients

Identification and Isolation of Insertion Sequences, in Carbapenem Resistant Clinical Isolates of Acinetobacter baumannii from Tehran Hospitals

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