Antibiotic Resistance and Plasmid Profile of Vibrio parahaemolyticus Strains Isolated from Kyunggi-Incheon Coastal Area

Abstract

Vibrio parahaemolyticus is one of the major agents responsible for food poisoning during summer in Korea, which is transmitted via seawater or seafoods. Recently, distribution of the bacteria in the marine environment has been increased due to global warming. Great concern also has been raised regarding public hygiene as well as marine culture by the emergence of pathogens with antibiotic resistance. Therefore, distribution of V. parahaemolyticus and antibiotic resistance of the isolates were monitored in 7 coastal areas of Kyonggi Province and Incheon by sampling seawater, fishes and clams monthly. V. parahaemolyticus was detected from 47.7% of 966 samples (seawater 61.9%, seafoods 41.8%) analyzed using CHROMagar TM and TCBS agar plates as well as multiplex PCR. Among 13 antibiotics tested, resistance to vancomycin and ampicillin was observed in 97.3% and 87.3% of the isolates, respectively, and the ratios of them resistant to cephalothin (48.8%) and rifampin (46.1%) were also high. The isolates were most highly sensitive to chloramphenicol (91.7%) and trimethoprim-sulfamethoxazole (91.8%). The ratio of sensitivity for other antibiotics was also high in the descending order of gentamycin (82.3%), tobramycin (74.8%), nalidixic acid (71.6%), tetracyclin (69.4%), cefotaxime (63.0%). About 69% of the isolates showed multiple drug resistance toward 3 antibiotics including vancomycin and ampicillin. Two of them exhibited resistance for 11 antibiotics used in this study. Plasmid profile analysis of the isolates with antibiotic resistance revealed that 55.1% of them retained plasmids of 24 different types. However, no clear inter-relationship between the resistance and the plasmid profile has been observed.

Food-borne outbreaks, distributions, virulence, and antibiotic resistance profiles of Vibrio parahaemolyticus in Korea from 2003 to 2016: a review

“…Another study was conducted in samples (n = 685) of fish and shellfish collected from retail seafood markets in Kyunggi province and Incheon city, Korea. In that study, the monthly detection rate of V. parahaemolyticus ranged from 30.5 to 61.5%; the highest level was found in August (Han et al 2012).…”

“…In some reported results, V. parahaemolyticus was enumerated by the most probable number method. Han et al (2012) investigated the abundance of V. parahaemolyticus in 281 seawater samples collected from the west coast of Korea (Kyunggi province and Incheon city). According to their results, the level of V. parahaemolyticus was high in summer, especially in August (92.9%) followed by July (85.0%) and September (76.5%).…”

“…V. parahaemolyticus isolates were resistant to ampicillin (96.9%), amikacin (29.2%), and tetracycline (27.7%). Han et al (2012) determined the antibiotic resistance profile of 716 V. parahaemolyticus strains isolated from samples of seawater, fish, and shellfish on seven coastal areas of Kyunggi province and Incheon city, Korea. According to their results, 97.3 and 87.3% of the isolates showed very high resistance to vancomycin and ampicillin, respectively, followed by cephalothin (48.8%) and rifampin (46.1%).…”

Genetic Relationship, Virulence Factors, Drug Resistance Profile and Biofilm Formation Ability of Vibrio parahaemolyticus Isolated From Mussel

“…Previous reports performed in South Korea showed the antibiotic susceptibility profile of V. parahaemolyticus isolates in seawater samples and found that 3.0–12.2% of isolates were resistant to tetracycline and ciprofloxacin, respectively ( Son et al, 2005 ; Kim et al, 2014 ). Other studies reported using different antibiotics against V. parahaemolyticus for detecting antibiotic susceptibility ( Han et al, 2012 ; Yang J. H. et al, 2017 ; Park et al, 2018 ). Most of the antimicrobials tested in this study are using in agriculture and aquaculture fields ( Kim S. et al, 2016 ; Kang et al, 2017 ), as well as in the treatment of vibrio infections ( Shaw et al, 2014 ).…”

Antimicrobial Resistance and Minimum Inhibitory Concentrations of Vibrio parahaemolyticus Strains Isolated from Gomso Bay, Korea

“…장염비브리오의 항균제 내성 양상 amikacin, ampicillin, cephalothin, cefoxitin, gentamicin, kanamycin, rifampin, streptomycin vancomycin (Son et al, 2005;Lee et al, 2009;Ryu et al, 2010;Han et al, 2012;Kim et al, 2014;Kang et al, 2016;Kim et al, 2016 (Tanil et al, 2005;Lee et al, 2009;Lee and Park, 2010;Kim et al, 2014). Clindamycin MIC 16.0 µg/…”