Antibiotic resistance and prevalence of β-lactamase in Haemophilus influenzae isolates—a surveillance study of patients with respiratory infection in Saudi Arabia☆

Abdel-Rahman, E. M.; Ismael, Nashat A.; Dixon, Ronald

doi:10.1016/s0732-8893(99)00142-x

Cited by 14 publications

(11 citation statements)

References 29 publications

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“…H. influenzae resistance to ␤-lactam antibiotics is an increasing problem. The resistance to ampicillin in this organism varies from 10% to 60%, depending on the geographical region, and is predominantly mediated by TEM-1 or ROB-1 ␤-lactamase production (1,11,18). Although it is rare in other countries, a high incidence of ␤-lactamase-negative, ampicillin-resistant (BLNAR) H. influenzae strains showing a decreased affinity of penicillin-binding protein 3 (PBP 3) for ␤-lactam antibiotics due to amino acid substitutions has been reported in Japan (15,19).…”

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confidence: 99%

Antimicrobial Resistance in Haemophilus influenzae Respiratory Tract Isolates in Korea: Results of a Nationwide Acute Respiratory Infections Surveillance

Bae

Lee

et al. 2010

Antimicrob Agents Chemother

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Antimicrobial susceptibility patterns and ␤-lactam resistance mechanisms of 544 Haemophilus influenzae isolates through the nationwide Acute Respiratory Infections Surveillance (ARIS) network in Korea during 2005 and 2006 were determined. Resistance to ampicillin was 58.5%, followed by resistance to cefuroxime (23.3%), clarithromycin (18.7%), cefaclor (17.0%), amoxicillin-clavulanate (10.4%), and chloramphenicol (8.1%). Levofloxacin and cefotaxime were the most active agents tested in this study. ␤-Lactamase production (52.4%) was the main mechanism of ampicillin resistance, affecting 96.1% of TEM-1-type ␤-lactamase. According to their ␤-lactam resistance mechanisms, all isolates were classified into the following groups: ␤-lactamase-negative, ampicillin-sensitive (BLNAS) strains (n ‫؍‬ 224; 41.5%); ␤-lactamase-positive, ampicillin-resistant (BLPAR) strains (n ‫؍‬ 255; 47.2%); ␤-lactamase-negative, ampicillin-resistant (BLNAR) strains (n ‫؍‬ 33; 6.1%); and ␤-lactamase-positive, amoxicillin-clavulanate-resistant (BLPACR) strains (n ‫؍‬ 28; 5.2%). Among the BLNAR and BLPACR strains, there were various patterns of multiple-amino-acid substitutions in penicillin-binding protein 3. Particularly, among BLNAR, group III isolates, which had three simultaneous substitutions (Met377Ile, Ser385Thr, and Leu389Phe), were identified for the first time in Korea. Three group III strains displayed the highest MIC of cefotaxime (1 to 2 g/ml). The results indicate the importance of monitoring a changing situation pertaining to the increase and spread of BLNAR and BLPACR strains of H. influenzae for appropriate antibiotic therapy for patients with respiratory tract infections in Korea.

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confidence: 99%

Antimicrobial Resistance in Haemophilus influenzae Respiratory Tract Isolates in Korea: Results of a Nationwide Acute Respiratory Infections Surveillance

Bae

Lee

et al. 2010

Antimicrob Agents Chemother

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“…In the previously reported study [7] 7.8% of the strains isolated overall were found to be encapsulated and none were recognised as non-b serotypes. All isolates were sub-cultured once onto chocolate agar slopes directly from the clinical material and serotyped before longer term storage procedures were undertaken to avoid possible in vitro changes in encapsulation or outer membrane composition.…”

Section: Susceptibility Testingmentioning

confidence: 66%

“…MIC values obtained previously [7] showed that most (80.6%) of isolates tested were sensitive to ampicillin. All of the isolates tested by agar MICs were fully susceptible to ceftazidime, and ciprofloxacin although 25 of the 129 isolates demonstrated reduced susceptibility to ampicillin (further details of the susceptibility of our strains of H. influenzae to various antimicrobials has been reported previously [7])…”

Section: Susceptibility Of Isolatesmentioning

confidence: 69%

“…All isolates were sub-cultured directly onto chocolate agar slopes and details of patients, etc. have been reported elsewhere [7]. All isolates were transferred to glycerol broth and stored at −70 • C.…”

Section: Bacterial Strainsmentioning

confidence: 99%

“…The distribution of isolates producing ␤-lactamase ranged from 10% in Jeddah (2 hospitals) to 14.6% in Riyadh (3 hospitals) (see Ref. [7]) Among the 17 ampicillin-resistant TEM-1 producing strains, 4 (23.5%) were encapsulated serotype b. Biotypes I, II and III accounted for 94.3% of the ␤-lactamase-positive strains; of these 35 and 47% belonged to biotypes I and II respectively. Table 1).…”

Section: Serotyping Biotyping and ␤-Lactamase Production By H Influmentioning

confidence: 99%

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Outer membrane protein analysis of ampicillin-resistant isolates of Haemophilus influenzae from Saudi Arabia

Dixon

Abdel-Rahman²

2008

Journal of Infection and Public Health

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Haemophilus influenzae isolates characterized in a previously published study from the Kingdom of Saudi Arabia were analysed by outer membrane protein (OMP) profiling. Isolates from patients with confirmed respiratory tract infections were investigated. Antibiotic susceptibility tests in vitro showed 25/129 (19.4%) had various degrees of reduced susceptibility to ampicillin although all were fully susceptible to ceftazidime and ciprofloxacin. OMP profiles of the beta-lactamase mediated ampicillin-resistant and beta-lactamase negative; ampicillin intermediate resistant strains (BLNAI) isolated were investigated. Dendrograms of scanned SDS-PAGE of these strains showed 15 different groupings from the 15 non-typable (NTHi) isolates tested demonstrating a high degree of heterogeneity whereas the 5 Hib isolates demonstrated significantly closer relatedness and were probably clonal. The present study demonstrates the groupings of H. influenzae strains by OMP profile analysis which did not correlate with the beta-lactamase production ability, BLNAI isolates, geographical origin or biotype.

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Antimicrobial Resistance: Preventable or Inevitable?

Aksoy

Tanrıöver

Ünal

2008

Antibiotic Policies: Fighting Resistance

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Antibiotic resistance and prevalence of β-lactamase in Haemophilus influenzae isolates—a surveillance study of patients with respiratory infection in Saudi Arabia☆

Cited by 14 publications

References 29 publications

Antimicrobial Resistance in Haemophilus influenzae Respiratory Tract Isolates in Korea: Results of a Nationwide Acute Respiratory Infections Surveillance

Antimicrobial Resistance in Haemophilus influenzae Respiratory Tract Isolates in Korea: Results of a Nationwide Acute Respiratory Infections Surveillance

Outer membrane protein analysis of ampicillin-resistant isolates of Haemophilus influenzae from Saudi Arabia

Antimicrobial Resistance: Preventable or Inevitable?

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