Antibiotic resistance and typing of the methicillin-resistant Staphylococcus aureus clones in Kuwait hospitals, 2016–2017

Boswihi, Samar S.; Udo, Edet E.

doi:10.1186/s12866-020-02009-w

Cited by 16 publications

(24 citation statements)

References 34 publications

(50 reference statements)

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“…Isolates of other CCs (CC5, CC97) and of spa-types assignable to other CCs were most frequently observed in meat from sheep in 2020 (5/11 isolates). CC97 isolates have previously been associated with bovines (K € appeli et al, 2019) and pigs in Italy (Feltrin et al, 2015), but they have also been observed in human cases unrelated to animal production (Monecke et al, 2011;Boswihi et al, 2020). Therefore, their origin in food is doubtful.…”

Section: Typing Results Of Mrsa From Animals and Foodmentioning

confidence: 99%

The European Union Summary Report on Antimicrobial Resistance in zoonotic and indicator bacteria from humans, animals and food in 2019–2020

2022

EFS2

132

119

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Data on antimicrobial resistance (AMR) in zoonotic and indicator bacteria from humans, animals and food are collected annually by the EU Member States (MSs), jointly analysed by the EFSA and the ECDC and reported in a yearly EU Summary Report. The annual monitoring of AMR in animals and food within the EU is targeted at selected animal species corresponding to the reporting year. The 2020 monitoring specifically focussed on poultry and their derived carcases/meat, while the monitoring performed in 2019 specifically focused on fattening pigs and calves under 1 year of age, as well as their derived carcases/meat. Monitoring and reporting of AMR in 2019–2020 included data regarding Salmonella, Campylobacter and indicator E. coli isolates, as well as data obtained from the specific monitoring of presumptive ESBL‐/AmpC‐/carbapenemase‐producing E. coli isolates. Additionally, some MSs reported voluntary data on the occurrence of methicillin‐resistant Staphylococcus aureus in animals and food, with some countries also providing data on antimicrobial susceptibility. This report provides an overview of the main findings of the 2019–2020 harmonised AMR monitoring in the main food‐producing animal populations monitored, in carcase/meat samples and in humans. Where available, monitoring data obtained from pigs, calves, broilers, laying hens and turkeys, as well as from carcase/meat samples and humans were combined and compared at the EU level, with particular emphasis on multidrug resistance, complete susceptibility and combined resistance patterns to critically important antimicrobials, as well as Salmonella and E. coli isolates possessing ESBL‐/AmpC‐/carbapenemase phenotypes. The key outcome indicators for AMR in food‐producing animals, such as complete susceptibility to the harmonised panel of antimicrobials in E. coli and the prevalence of ESBL‐/AmpC‐producing E. coli have been specifically analysed over the period 2014–2020.

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Section: Typing Results Of Mrsa From Animals and Foodmentioning

confidence: 99%

The European Union Summary Report on Antimicrobial Resistance in zoonotic and indicator bacteria from humans, animals and food in 2019–2020

2022

EFS2

132

119

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“…MRSA has been mentioned in the EU/EEA (EARS-Net) Annual Epidemiological Report for 2019 as one of the most common causes of bloodstream infections, exhibiting a high burden in terms of morbidity and mortality [44]. These strains have become a severe clinical and epidemiological problem, as resistance to methicillin implies resistance to all β-lactam antibiotics [45]. The violet crystal quantification of biofilm formed on polystyrene surfaces in the presence of different concentrations of the complexes showed that both compounds were able to inhibit biofilm formation at the initial stage by reducing the expression of adhesion proteins, with the minimal biofilm eradication concentration (MBEC) values ranging from 0.71 to 0.04 mM (Table 7).…”

Section: Microbiological Assaymentioning

confidence: 99%

Biological Activity of Triazolopyrimidine Copper(II) Complexes Modulated by an Auxiliary N-N-Chelating Heterocycle Ligands

et al. 2021

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Novel complexes of type [Cu(N-N)(dmtp)2(OH2)](ClO4)2·dmtp ((1) N-N: 2,2′-bipyridine; (2) L: 1,10-phenantroline and dmtp: 5,7-dimethyl-1,2,4-triazolo[1,5-a]pyrimidine) were designed in order to obtain biologically active compounds. Complexes were characterized as mononuclear species that crystallized in the space group P-1 of the triclinic system with a square pyramidal geometry around the copper (II). In addition to the antiproliferative effect on murine melanoma B16 cells, complex (1) exhibited low toxicity on normal BJ cells and did not affect membrane integrity. Complex (2) proved to be a more potent antimicrobial in comparison with (1), but both compounds were more active in comparison with dmtp—both against planktonic cells and biofilms. A stronger antimicrobial and antibiofilm effect was noticed against the Gram-positive strains, including methicillin-resistant Staphylococcus aureus (MRSA). Both electron paramagnetic resonance (EPR) and Saccharomyces cerevisiae studies indicated that the complexes were scavengers rather than reactive oxygen species promoters. Their DNA intercalating capacity was evidenced by modifications in both absorption and fluorescence spectra. Furthermore, both complexes exhibited nuclease-like activity, which increased in the presence of hydrogen peroxide.

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“…Molecular typing tools including staphylococcal protein A (spa) typing, multilocus sequence typing (MLST), pulsed-field gel electrophoresis, staphylococcal cassette chromosome mec (SCC mec ) typing, DNA microarray, and whole genome sequencing (WGS) have been used in the epidemiologic surveillance of MRSA strains to detect and monitor emerging and reemerging infections as well as monitoring geographic spread and shifts of epidemic and endemic clones ( Pfaller, 1999 ; van Belkum et al, 2001 ; Monecke et al, 2011 ; Boswihi et al, 2016 , 2020a , 2020b ; Rebic et al, 2016 ). The application of these typing techniques to type MRSA from different geographic regions have shown that most of the MRSA infections reported worldwide were caused by a limited number of pandemic MRSA clones belonging to clonal complexes 5 (CC5), CC8/ST239, CC22, CC30, and CC45 ( Robinson and Enright, 2003 ; Monecke et al, 2011 ; Guthrie et al, 2020 ) although CA-MRSA isolates belong to more diverse genetic backgrounds compared to HA-MRSA ( Monecke et al, 2011 ; Udo, 2013 ; Tong et al, 2015 ; Guthrie et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

“…Methicillin-resistant Staphylococcus aureus strains belonging to CC361 (CC361-MRSA) were described as rare and were reported sporadically in humans ( Afroz et al, 2008 ; Weber et al, 2010 ; Coombs et al, 2011 ; Monecke et al, 2011 ; Shambat et al, 2012 ; Kinnevey et al, 2014 ), Rhesus monkeys ( Roberts et al, 2019 ), Cattle ( Tegegne et al, 2017 ), and in ready-to-eat food items ( Islam et al, 2019 ). However, the CC361-MRSA have been increasingly isolated from human patients in the Arabian Gulf countries of Saudi Arabia ( Senok et al, 2019 ), United Arab Emirates ( Senok et al, 2020 ) and Kuwait ( Boswihi et al, 2018 , 2020a , 2020b ).…”

Section: Introductionmentioning

confidence: 99%

“…The first two known isolates of CC361-MRSA were isolated in Kuwait in 2010 from two patients in two different hospitals ( Boswihi et al, 2016 ). Since then, the proportion of MRSA isolates belonging to CC361-MRSA has been increasing annually ( Boswihi et al, 2018 , 2020a , 2020b ). In this study, we investigated CC361-MRSA isolated from patients in public hospitals in Kuwait from 1 January, 2016 to 31 December, 2018, using staphylococcal protein A ( spa ) typing, multi-locus sequence typing (MLST) and DNA microarray to determine their antibiotic resistance and virulence profiles, and genetic relatedness.…”

Section: Introductionmentioning

confidence: 99%

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The Dissemination and Molecular Characterization of Clonal Complex 361 (CC361) Methicillin-Resistant Staphylococcus aureus (MRSA) in Kuwait Hospitals

et al. 2021

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Methicillin-resistant Staphylococcus aureus (MRSA) belonging to clonal complex 361 (CC361-MRSA) is rare among patients’ populations globally. However, CC361-MRSA has been isolated with an increasing trend among patients in Kuwait hospitals since 2010. This study investigated the molecular characteristics of CC361-MRSA isolated from patients in Kuwait hospitals in 2016–2018 to understand their genetic relatedness and virulence determinants. Of 5,223 MRSA isolates investigated by DNA microarray, 182 (3.4%) isolates obtained in 2016 (N = 55), 2017 (N = 56), and 2018 (N = 71) were identified as CC361-MRSA. The CC361-MRSA isolates were analyzed further using antibiogram, spa typing and multi locus sequence typing (MLST). Most of the isolates were resistant to fusidic acid (64.8%), kanamycin (43.4%), erythromycin (36.3%), and clindamycin (14.3%) encoded by fusC, aphA3, and erm(B)/erm(C) respectively. Nine isolates (4.9%) were resistant to linezolid mediated by cfr. The isolates belonged to 22 spa types with t3841 (N = 113), t315 (N = 16), t1309 (N = 14), and t3175 (N = 5) constituting 81.3% of the spa types, four genotypes (strain types), CC361-MRSA-[V/VT + fus] (N = 112), CC361-MRSA-IV, WA MRSA-29 (N = 36), CC361-MRSA-V, WA MRSA-70/110 (N = 33) and CC361-MRSA-[V + fus] variant (N = 1). MLST conducted on 69 representative isolates yielded two sequence types: ST361 (11/69) and ST672 (58/69). All CC361-MRSA isolates were positive for cap8, agr1, and the enterotoxin egc gene cluster (seg, sei, selm, seln, selo, and selu). The tst1 was detected in 19 isolates. The immune evasion cluster (IEC) genes type B (scn, chp, and sak) and type E (scn and sak) were detected in 20 and 152 isolates, respectively. The CC361-MRSA circulating in Kuwait hospitals consisted of two closely related sequence types, ST361 and ST672 with ST672-MRSA [V/VT + fus] as the dominant genotype. The dissemination of these newly emerged clones and the emergence of linezolid resistance limits therapeutic options, as well as present significant challenges for the control of MRSA infections in Kuwait hospitals.

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Antibiotic resistance and typing of the methicillin-resistant Staphylococcus aureus clones in Kuwait hospitals, 2016–2017

Cited by 16 publications

References 34 publications

The European Union Summary Report on Antimicrobial Resistance in zoonotic and indicator bacteria from humans, animals and food in 2019–2020

The European Union Summary Report on Antimicrobial Resistance in zoonotic and indicator bacteria from humans, animals and food in 2019–2020

Biological Activity of Triazolopyrimidine Copper(II) Complexes Modulated by an Auxiliary N-N-Chelating Heterocycle Ligands

The Dissemination and Molecular Characterization of Clonal Complex 361 (CC361) Methicillin-Resistant Staphylococcus aureus (MRSA) in Kuwait Hospitals

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