Antibiotic-resistance, enterotoxin gene profiles and farm-level prevalence of Staphylococcus aureus in cow, sheep and goat bulk tank milk in Jordan

Obaidat, Mohammad M.; Roess, Amira; Mahasneh, Amjad; Al-Hakimi, Rana A.

doi:10.1016/j.idairyj.2018.02.001

Cited by 13 publications

(11 citation statements)

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“…The high level of penicillin resistance observed in this study is consistent with many other authors (Pereira et al 2009;Jamali et al 2015;Chaalal et al 2018;Gharsa et al 2019;Obaidat et al 2018;Papadopoulos et al 2019b). The emergence and spread of antimicrobial resistance has been usually attributed to the misuse or indiscriminate use of antibiotics as therapeutic drugs in human and animal health care or as growth promoters in veterinary husbandry (Ruiz and Alvarez-Ord oñez 2017).…”

Section: Discussionsupporting

confidence: 93%

“…However, the prevalence obtained in this study was higher than that previously observed by Mairi et al (2019), with rate of 8Á6%. A higher prevalence of S. aureus have been observed in other countries concerning various foodstuffs, including raw milk and dairy products (Rola et al 2015;Mehli et al 2017;Obaidat et al 2018;Ahmed Abdel-Hameid et al 2019;Papadopoulous et al2019b), meat and meat products (Tang et al 2017;Wu et al 2018) and ready to eat products (Islam et al 2019). The differences between results obtained from various studies about prevalence rates of S. aureus may be related to different attributes, including sources of samples, geographical origin, inappropriate antimicrobial administrations, sensitivity of the identification methods, sample size, storage and handling of samples (Al-Ashmawy et al 2016;Gharsa et al 2019).…”

Section: Discussionmentioning

confidence: 95%

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Enterotoxin genes and antimicrobial resistance in Staphylococcus aureus isolated from food products in Algeria

Titouche

Houali

Ruiz-Ripa

et al. 2020

J. Appl. Microbiol.

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Aims The aim of this study was to characterize Staphylococcusaureus isolates of food origin (dairy and meat products, pastries and sandwiches) determining the carriage in enterotoxin genes and the antimicrobial resistance pheno/genotypes. Methods and results A total of 300 food samples were collected and analysed for the detection of S. aureus. The presence of enterotoxin genes was investigated by multiplex PCRs. Resistance of isolates to 11 antimicrobials was determined using disc diffusion method and molecular characterization of methicillin‐resistant S. aureus was carried out by spa typing and multilocus sequence typing. Overall, 51 out of 300 samples (17%) were contaminated with S. aureus, and 104 isolates were recovered. In all, 65 of these isolates (62·5%) harboured one or more genes encoding for staphylococcal enterotoxins, being seg and sei the most observed genes. The highest resistance profile was ascribed to penicillin G (95·19%). Five isolates were methicillin‐resistant (MRSA) harbouring the mecA gene. All MRSA isolates belonged to the sequence type ST5 and to two different spa types (t450 and t688); the MRSA‐t450 isolate carried the scn gene (specific marker of the immune evasion cluster system), but the four MRSA‐t688 isolates were scn negative. The MRSA isolates carried enterotoxin genes but were negative for the genes of the Panton Valentine leukocidine (lukF/S‐PV). Conclusion The presence of enterotoxigenic S. aureus isolates, including MRSA, in food samples can represent a risk for public health. Significance and Impact of this Study This work describes the molecular characteristics of MRSA strains isolated from foods in Algeria and it can contribute to an extended database concerning the S. aureus isolated from food origin.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 95%

Enterotoxin genes and antimicrobial resistance in Staphylococcus aureus isolated from food products in Algeria

Titouche

Houali

Ruiz-Ripa

et al. 2020

J. Appl. Microbiol.

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“… Pexara et al (2016) reported that no S. aureus isolates in caprine milk carry the seb gene. Obaidat et al (2018) also did not detect the seb gene and only 0.6% of the sed gene was detected in Jordan’s BTM. Different studies have reported different prevalence rates of the virulence genes, which may be attributed to geographical distribution and the sources of the samples ( Zhao et al, 2020 ).…”

Section: Discussionmentioning

confidence: 95%

Prevalence and Characteristics of Staphylococcus aureus Isolated From Retail Raw Milk in Northern Xinjiang, China

et al. 2021

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Staphylococcus aureus is one of the main pathogens causing mastitis in dairy animals worldwide. It is an important opportunistic pathogen of raw milk, and the enterotoxin causes significant food poisoning. Monitoring the antibiotic resistance of S. aureus in raw milk is helpful for a risk assessment of S. aureus. In this study, 62 strains (43.1%) of S. aureus were isolated from 144 retail raw milk samples of different varieties from four regions in northern Xinjiang, China. Among them, the isolation rates at Shihezi, Hami, Altay, and Tacheng were 58.1% (54/93), 12.9% (4/31), 18.2% (2/11), and 22.2% (2/9), respectively. The isolation rate of positive strains in cow milk samples was the highest (61.7%, 37/60), followed by camel milk (35.9%, 23/64), and horse milk (10.0%, 2/20). The results of the classical virulence genes test showed that 12.9% (8/62) of the isolates carried at least one virulence gene. The main genotype was see (6.5%, 4/62), followed by sea+sec (3.2%, 2/62), sea (1.6%, 1/62), and sec (1.6%, 1/62). The analysis of 13 resistance genes and the susceptibility to 12 different antibiotics of 62 isolates showed that 80.6% (50/62) of the strains were resistant to at least one antibiotic, and 46.8% (29/62) were resistant to three or more antibiotics. The isolated strains had the highest resistance rate to penicillin (72.6%, 45/62), and 25.8% (16/62) of the isolates carried the blaZ resistance gene. In addition, 32 strains (51.6%, 32/62) of methicillin-resistant S. aureus were detected. All isolates had the ability to form biofilms. The pulsed-field gel electrophoresis results showed that the 47 isolates revealed 13 major pulsotypes (P1–P13) and 26 subtypes with 80% similarity, indicating the overall genetic diversity in the distribution area and sources of the samples. These findings indicate that S. aureus causes serious pollution of raw milk in northern Xinjiang, which has a negative effect on public health. Therefore, control measures and continuous monitoring should be undertaken to ensure the quality and safety of raw milk.

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“…For example, in Pakistan, Altaf et al [6] reported that 19% of 122 S. aureus recovered from the milk of goats showed resistance to methicillin, whilst Caruso et al [7] reported that they recovered only one such isolate from bulk-tank milk of 66 goat farms in Italy. In research that evaluated more antibiotics, the proportion of resistant isolates was found to be up to 100% in Brazil [8] and Jordan [9]. Thus far, all studies related to the resistance of staphylococci from goat milk have focused on the patterns of resistance of the staphylococcal isolates; herd management factors that are potentially associated with the development of resistance have not been thoroughly studied.…”

Section: Introductionmentioning

confidence: 99%

Prevalence, Patterns, Association with Biofilm Formation, Effects on Milk Quality and Risk Factors for Antibiotic Resistance of Staphylococci from Bulk-Tank Milk of Goat Herds

et al. 2021

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The objectives of this work were to study the prevalence and the patterns of antibiotic resistance of staphylococcal isolates from bulk-tank milk of goat herds across Greece, to assess possible associations of the presence of antibiotic resistance with the quality of milk in these herds and to evaluate herd-related factors potentially associated with the presence of antibiotic resistance among these staphylococcal isolates. A cross-sectional study was performed on 119 goat herds in Greece. Bulk-tank milk samples were collected for bacteriological examination; staphylococcal isolates were evaluated for resistance to 20 antibiotics. Oxacillin-resistant, resistant to at least one antibiotic, and multi-resistant staphylococcal isolates were recovered from 5.0%, 30.3%, and 16.0% of herds, respectively. Of 80 isolates, 7.5% were resistant to oxacillin, 50.0% were resistant to at least one antibiotic and 27.5% were multi-resistant. Resistance was seen more frequently among coagulase-negative staphylococci (59.3%) than among Staphylococcus aureus (23.8%). Resistance was more frequent against penicillin and ampicillin (41.3% of isolates) and fosfomycin (27.5%). No association was found with biofilm formation by staphylococci. For recovery of oxacillin-resistant isolates, the presence of working staff in the herds emerged as a significant factor; respective factors for the isolation of staphylococci resistant to at least one antibiotic were part-time farming and high (>10) number of systemic disinfections in the farm annually. The same three factors concurrently were also identified to be significant for the recovery of multi-resistant isolates.

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Antibiotic-resistance, enterotoxin gene profiles and farm-level prevalence of Staphylococcus aureus in cow, sheep and goat bulk tank milk in Jordan

Cited by 13 publications

References 61 publications

Enterotoxin genes and antimicrobial resistance in Staphylococcus aureus isolated from food products in Algeria

Enterotoxin genes and antimicrobial resistance in Staphylococcus aureus isolated from food products in Algeria

Prevalence and Characteristics of Staphylococcus aureus Isolated From Retail Raw Milk in Northern Xinjiang, China

Prevalence, Patterns, Association with Biofilm Formation, Effects on Milk Quality and Risk Factors for Antibiotic Resistance of Staphylococci from Bulk-Tank Milk of Goat Herds

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