Antibiotic resistance genes and their links with bacteria and environmental factors in three predominant freshwater aquaculture modes

Wang, Qidong; Mao, Chengzhi; Lei, Lei; Yan, Biao; Yuan, Jing; Guo, Yongyong; Li, Tianli; Xiong, Xiong; Cao, Xiuyun; Huang, Jie; Han, Jian; Yu, Ke; Zhou, Bingsheng

doi:10.1016/j.ecoenv.2022.113832

Cited by 23 publications

(10 citation statements)

References 57 publications

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“…Aurantimicrobium and Rhodoluna had higher relative abundances in the 0 and 4 h samples, indicating that the high concentrations of SAs and SA products did not have a strong influence on these genera. The antibiotic resistance genes present in river water are not negligible; Aurantimicrobium and Rhodoluna may carry antibiotic resistance genes, indicating their potential for greater resistance than other bacteria in our SA effluent samples. The relative abundance of Rhodoferax was correlated with the concentrations of SAs and SA products.…”

Section: Resultsmentioning

confidence: 94%

Degradation of Sulfonamides by UV/Electrochemical Oxidation and the Effects of Treated Effluents on the Bacterial Community in Surface Water

et al. 2023

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This study evaluated the effects of ultraviolet (UV) photolysis combined with electrochemical oxidation on sulfonamides (SAs) as well as its treated effluent on the bacterial community in surface water. In terms of degradation rate, the best anode material for electrochemical oxidation was Ti/RuO2–IrO2, which had the highest degradation kinetic constant compared to Ti/Ta2O5–IrO2 and Ti/Pt. Experiments showed the highest degradation rate of SAs at 8.3 pH. Similarly, increasing the current leads to stronger degradation due to the promotion of free chlorine production, and its energy consumption rate decreases slightly from 73 to 67 W h/mmol. Compared with tap water, the kinetic constants decreased by 20–62% for SAs in three different surface water samples, which was related to the decrease in free chlorine. When extending the reaction time to 24 h, the concentrations of chemical oxygen demand and total organic carbon decreased by approximately 30–40%, indicating that the SAs and their products could be mineralized. The diversity analysis showed that the effluents influenced the richness and diversity of the bacterial community, particularly in the 4 h sample. Additionally, there were 86 operational taxonomic units common to all samples, excluding the 4 h sample; significant differences were derived from changes in the Actinobacteriota and Bacteroidota phyla. The toxicity of the products might explain these changes, and these products could be mineralized, as observed in the 24 h sample. Therefore, the extension of treatment time would greatly reduce the ecological harm of treated effluent and ensure that the UV/electrochemical process is a feasible treatment option. Overall, this study provides valuable insight into the optimization and feasibility of UV/electrochemical processes as a sustainable treatment option for sulfonamide-contaminated water sources, emphasizing the importance of considering ecological impacts and the need for extended treatment times that address environmental concerns and ensuring improved water quality.

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Section: Resultsmentioning

confidence: 94%

Degradation of Sulfonamides by UV/Electrochemical Oxidation and the Effects of Treated Effluents on the Bacterial Community in Surface Water

et al. 2023

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“…Concentrations of the sulfonamide resistance gene sul1 and tetracycline resistance gene tetW were the highest in 15 sediment samples collected from five Chinese provinces (Cheng et al, 2021). In addition, the quinolone resistance gene qnrB is abundant in six freshwater ponds (Wang et al, 2022). Integrons play an important role in the emergence and horizontal transfer of ARGs among bacteria and are the main transmissible vectors commonly found in different environments (Wang et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

“…Environmental factors such as total phosphorus and total nitrogen affect the abundance and diversity of ARGs in shrimp and fishponds (Su et al, 2017). Furthermore, bacterial communities in different aquaculture modes affect the distribution and abundance of ARGs (Huang et al, 2017;Wang et al, 2022). These findings indicated that antibiotic use and environmental factors affect the occurrence and distribution of ARGs.…”

Section: Introductionmentioning

confidence: 99%

High occurrence of antibiotic resistance genes in intensive aquaculture of hybrid snakehead fish

Lin

Tan²,

Deng³

et al. 2023

Front. Mar. Sci.

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The increasing abundance of antibiotic resistance genes (ARGs), which are regarded as new pollutants, has raised public health concern. The use of antibiotics in aquaculture has promoted the evolution and spread of ARGs. The occurrence and abundance of ARGs in aquaculture has attracted extensive attention. However, the distribution and transmission of ARGs in aquaculture require further study. This study analyzed water and sediment from intensive culture of hybrid snakehead fish farm in Zhongshan, South China. Twenty-two types of ARGs were detected in all environmental samples. The relative abundance of sulfonamide resistance genes (sul1 and sul2) was the highest, ranging from 3.37×10-2 to 8.53×100 copies/16S rRNA gene. High occurrence of quinolones, phenicols, tetracycline resistance genes, and class 1 integrase gene (intI1) was also observed in pond water samples. This implies that pond water is one of the main reservoirs and origins of ARGs in the aquatic environment. Proteobacteria was the most abundant phylum in all the environmental samples, and its relative abundance ranged from 24.05% to 41.84%. Network and canonical correspondence analyses showed that a high abundance of ARGs (int1, sul1, sul2, qacEΔ1, aac6, and oqxA) was positively correlated with Proteobacteria, Cyanobacteria, and Bacteroidetes, and the abundance of Proteobacteria, Actinobacteria, and Patescibacteria was positively correlated with environmental factors (sulfide, nitrite nitrogen, pH, free chlorine, and ammonia nitrogen). These findings demonstrate the prevalence and persistence of ARGs in intensive fish farming in southern China. This suggests that ARG levels and microbiological community composition in aquaculture should be conventionally determined to assess potential risks to public health.

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“…The presence of ARGs in freshwater lakes is considered a hazardous issue for ecological and human health (Squadrone 2020a, Komijani et al 2021a, Ana et al 2021, Wang et al 2022a). These lakes should be of the highest priority since they are vulnerable to diverse anthropogenic activities such as fishing, swimming, etc These activities could cause long-term damage, requiring remediation measures that are cost-ineffective.…”

Section: Implications For the Freshwater Lake Ecological And Human He...mentioning

confidence: 99%

Sulfonamide and tetracycline resistance genes in nanjing lakes: effect of water quality and heavy metals

Rajasekar

Zhao

Norgbey

et al. 2023

Environ. Res. Commun.

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Due to high population density, anthropogenic activities and waste disposal have negatively affected artificial lakes in urban areas. The lakes capacity for auto-depuration is limited since it is affected by overwhelming anthropogenic pollution; thus, they have the potential to act as reservoirs for antibiotic resistance genes (ARGs). In this study, we examined three urban artificial freshwater lakes in Nanjing to determine the abundance of sulfonamide and tetracycline resistance genes. Concerning water quality, the three lakes were found to be highly eutrophic, owing to their high levels of Total Nitrogen (TN), Phosphorous (TP), and Chlorophyll a (Chla). The average abundance of sulfonamide resistance genes detected in the three urban lakes was 42.446 log10 gene copies/100 mL, which was lower than the average abundance of tetracycline resistance genes (68.207 log10 gene copies/100 mL). Analysis by ANOVA revealed that all ARGs, except sul3, showed significant differences, probably due to varied anthropogenic influences in lakes. Pearson correlation and principal component analyses were performed to explore the correlation between ARGs, water quality markers, and heavy metals to understand the co-selection and drivers of ARGs propagation. tetM showed no correlation with any water quality markers, whereas Chla showed a positive correlation with all ARGs except tetM. tetM was the only gene that was unaffected by TN, TP, and Chla. The tet genes also showed strong associations with each other except tetM, especially tetA, tetQ, and tetG. The co-selection results between most heavy metals and ARGs were insignificant (p>0.05), with tetM being the most sensitive to the effects of heavy metals and Arsenic (As) having the strongest effect on sul3 and tet genes. The results from this study provide basic but archival information that TN, Chla and As, might be directly or indirectly involved in the dissemination of certain sulfonamide and tetracycline ARGs in freshwater environments.

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Antibiotic resistance genes and their links with bacteria and environmental factors in three predominant freshwater aquaculture modes

Cited by 23 publications

References 57 publications

Degradation of Sulfonamides by UV/Electrochemical Oxidation and the Effects of Treated Effluents on the Bacterial Community in Surface Water

Degradation of Sulfonamides by UV/Electrochemical Oxidation and the Effects of Treated Effluents on the Bacterial Community in Surface Water

High occurrence of antibiotic resistance genes in intensive aquaculture of hybrid snakehead fish

Sulfonamide and tetracycline resistance genes in nanjing lakes: effect of water quality and heavy metals

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