Antibiotic resistance genes in different animal manures and their derived organic fertilizer

Xu, Yan; Li, Houyu; Shi, Rongguang; Lv, Jiapei; Li, Bihan; Yang, Fengxia; Zheng, Xiangqun; Xu, Jian

doi:10.21203/rs.3.rs-29643/v2

Cited by 3 publications

(6 citation statements)

References 32 publications

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“…Its CTC resistance was consistent with previous studies [31]. Composting generally had a heterogeneous population composed of animal and human pathogens that could cause disease in livestock [32,33]. These ndings suggested that CTC increased the risk of human pathogenic bacteria circulating in the environment and posed a threat to human health.…”

Section: Resultssupporting

confidence: 88%

“…5). It was reported that Corynebacterium_1, a pathogenic bacterium, was a major genus in animal manure and organic fertilizer [32,33]. It was also found to resistant to CTC.…”

Section: Resultsmentioning

confidence: 99%

“…In the processing of composting, pathogenic bacteria (Corynebacterium_1, Acinetobacter, and Enterococcus) could not be completely removed, and the existence of antibiotics led to a partial proliferation. Pathogenic bacteria were signi cantly correlated with ARGs, indicating that pathogenic bacteria could become a major host of ARGs [32,46,47]. Enterococcus was correlated positively with tetX.…”

Section: Analysis Of Differences In Tcb and The Relationship Between Arb And Argsmentioning

confidence: 99%

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Distribution of Antibiotic-resistant Bacteria in Aerobic Composting of Swine Manure With Different Antibiotics

Song

et al. 2021

Preprint

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Background: Livestock manure is an important reservoir of antibiotic-resistant bacteria (ARB) and antibiotic resistance genes (ARGs). The community structure and bacterial diversity are usually studied using high-throughput sequencing that cannot provide direct evidence for ARB changes. Thus, little is known about the distribution of ARB, especially in the presence of different antibiotics in composting process. In this study, the fate of ARB was investigated in aerobic composting of swine manure, using chlortetracycline, sulfamethoxazole, lincomycin, ciprofloxacin as typical antibiotics. The abundance and species of ARB were analyzed systematically to evaluate their ecological risk at different stages of composting. Results: The absolute abundance of total ARB decreased while the relative abundance showed an increasing trend on Day 2. The relative abundance of lincomycin-resistant bacteria was higher than other ARBs during the whole composting process. The absolute abundance of four ARBs was 9.42×106-2.51×102 CFU/g (lincomycin- > chlortetracycline- > sulfamethoxazole- > ciprofloxacin- > multiple antibiotic-resistant bacteria), and they were not completely inactivated at the end of composting. Antibiotics led to a partial proliferation of ARBs including Corynebacterium_1, Sporosarcina, Solibacillus, and Acinetobacter. Especially, Corynebacterium_1, a pathogenic bacterium, was observed in the treatments of chlortetracycline and lincomycin. Conclusion: Among the antibiotics studied, lincomycin showed the highest ecological risk, due to it expanded the range of lincomycin-resistant bacteria at the phyla level (Firmicutes, Actinobacteria, and Proteobacteria). The principal co-ordinates analysis indicated that the bacterial community structure was primarily associated with the composting stages rather than the antibiotic types. Possible potential hosts and degrading bacteria for ARGs were indicated based on the network analysis. The decrease of culturable Proteobacteria and the increase of culturable Firmicutes (Solibacillus, Bacillus) partially explained the high removal rate of various ARGs in this study. These results provided important information for the control of antibiotic resistance in composting.

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Section: Resultssupporting

confidence: 88%

“…5). It was reported that Corynebacterium_1, a pathogenic bacterium, was a major genus in animal manure and organic fertilizer [32,33]. It was also found to resistant to CTC.…”

Section: Resultsmentioning

confidence: 99%

Section: Analysis Of Differences In Tcb and The Relationship Between Arb And Argsmentioning

confidence: 99%

See 1 more Smart Citation

Distribution of Antibiotic-resistant Bacteria in Aerobic Composting of Swine Manure With Different Antibiotics

Song

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…Its CTC resistance was consistent with previous studies [31]. Composting generally had a heterogeneous population composed of animal and human pathogens that could cause disease in livestock [32,33]. These findings suggested that CTC increased the risk of human pathogenic bacteria circulating in the environment and posed a threat to human health.…”

Section: T5supporting

confidence: 88%

“…5). It was reported that Corynebacterium, a pathogenic genus, was a major genus in animal manure and organic fertilizer [32,33]. It was also found to resistant to CTC.…”

Section: T5mentioning

confidence: 99%

Distribution of antibiotic-resistant bacteria in aerobic composting of swine manure with different antibiotics

Song

et al. 2021

Environ Sci Eur

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Background Livestock manure is an important reservoir of antibiotic-resistant bacteria (ARB) and antibiotic-resistance genes (ARGs). The bacterial community structure and diversity are usually studied using high-throughput sequencing that cannot provide direct evidence for ARB changes. Thus, little is known about the distribution of ARB, especially in the presence of different antibiotics in composting process. In this study, the fate of ARB was investigated in aerobic composting of swine manure, using chlortetracycline, sulfamethoxazole, lincomycin, and ciprofloxacin as typical antibiotics. The abundance and species of ARB were analyzed systematically to evaluate their ecological risk at different stages of composting. Results The absolute abundance of total ARB decreased, while the relative abundance increased on day 2. The relative abundance of lincomycin-resistant bacteria was higher than other ARBs during the whole composting process. The absolute abundance of four ARBs was 9.42 × 106–2.51 × 102 CFU/g (lincomycin- > chlortetracycline- > sulfamethoxazole- > ciprofloxacin- > multiple antibiotic-resistant bacteria), and they were not completely inactivated at the end of composting. Antibiotics led to a partial proliferation of ARBs including Corynebacterium, Sporosarcina, Solibacillus, and Acinetobacter. Especially, Corynebacterium, a pathogenic genus, was observed in chlortetracycline and lincomycin treatments. Conclusion Among the antibiotics studied, lincomycin showed the highest ecological risk, due to it expanded the range of lincomycin-resistant bacteria at the phyla level (Firmicutes, Actinobacteria, and Proteobacteria). The principal co-ordinates analysis indicated that the bacterial community structure was primarily associated with the composting stages rather than antibiotic types. Possible potential hosts and the related to the decrease of ARGs abundance were indicated based on the network analysis. The decrease of culturable Proteobacteria and the increase of culturable Firmicutes (Solibacillus, Bacillus) partially explained the high degradation rate of various ARGs with the progress of composting in this study. These results provided important information for the control of antibiotic resistance in composting.

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Antibiotic Resistant Escherichia coli Isolated from Faecal Samples of Cow at Livestock Farm in Kedah, Malaysia

Mohamed Suffian,

Nor Amdan,

Bakon

et al. 2023

MJMHS

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Introduction: Escherichia coli is a typical microflora found in the intestines of livestock, but regular exposure to antibiotics puts them under selection pressure to acquire antibiotic resistance. This study aimed to identify and characterise the antibiotic resistance profile of E. coli isolated in cow faeces collected from Tobiar Modern farm located in Kedah, Malaysia. Materials and methods: The antibiotic susceptibility test (AST) was conducted to assess the sensitivity of E. coli isolates to tetracycline (30 µg/mL), erythromycin (15 µg/mL) and ampicillin (10 µg/mL) using disk diffusion method followed by Minimum Inhibitory Concentration (MIC) assay. For molecular identification of selected resistant isolates, 16S rDNA gene sequencing was carried out. Results: Six (Isolates A1, P1, P2, P3, P4, and P5) out of 30 isolates were identified as E. coli based on their colonial morphological characteristics. The tests for catalase, indole, MR, TSI, and lactose fermentation all yielded positive results for the isolates, whereas the tests for oxidase, citrate, and VP yielded negative results. All six isolates were found to be erythromycin resistant. The Isolate P4 was observed as a multidrug resistant (MDR) bacterial strain since it exhibited resistance to all tested antibiotics. The MDR Isolate P4 is identified as E. coli strain LWY24 using molecular identification with a 99.7% identity rate. Conclusion: This study offers important preliminary information on the incidence of antibiotic-resistant bacteria (ARB) on this particular local livestock farm. This data is useful for developing plans to reduce the prevalence of ARB in livestock.

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Antibiotic resistance genes in different animal manures and their derived organic fertilizer

Cited by 3 publications

References 32 publications

Distribution of Antibiotic-resistant Bacteria in Aerobic Composting of Swine Manure With Different Antibiotics

Distribution of Antibiotic-resistant Bacteria in Aerobic Composting of Swine Manure With Different Antibiotics

Distribution of antibiotic-resistant bacteria in aerobic composting of swine manure with different antibiotics

Antibiotic Resistant Escherichia coli Isolated from Faecal Samples of Cow at Livestock Farm in Kedah, Malaysia

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