Antibiotic resistance in Campylobacter jejuni and Campylobacter coli isolated from food in Poland

Maćkiw, Elżbieta; Korsak, Dorota; Rzewuska, K.; Tomczuk, K.; Rozynek, E

doi:10.1016/j.foodcont.2011.08.022

Cited by 59 publications

(57 citation statements)

References 43 publications

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“…Furthermore, other studies con- cerning the prevalence of Campylobacter at the retail level showed a lower proportion of positive samples than obtained in the present investigations (Prencipe et al 2007;Lynch et al 2011). On the other hand, there are some reports showing a higher rate contamination in similar samples, even over 90% (Rozynek et al 2008;Tang et al 2009;Mackiw et al 2012). Additionally, the number of Campylobacter in the tested samples was estimated and it was noted that 20.2% of them were contaminated with bacteria at an enumerable level, i.e.…”

Section: Discussioncontrasting

confidence: 43%

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Prevalence, antimicrobial resistance, and molecular characterization of Campylobacter jejuni and C. coli isolated from retail raw meat in Poland

Wieczorek¹,

Szewczyk²,

Osek³

2012

Vet. Med.

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ABSTRACT:The study was conducted to investigate the presence of Campylobacter spp. in meat sold to consumers at a retail market in Poland. Antimicrobial resistance and the presence of putative virulence genes of the isolates were also examined. A total of 558 meat samples, including beef (n = 105), pork (n = 85), and poultry (n = 368) were collected over an almost three year study period. It was found that 321 samples, all of them originating from poultry, were contaminated with Campylobacter spp. Most of the obtained isolates were classified as C. coli (189 strains, 58.9%), whereas C. jejuni was identified in 132 (41.1%) samples. All Campylobacter strains were susceptible to gentamicin and all but one C. coli isolate to erythromycin. On the other hand, the highest level of resistance among Campylobacter tested was to ciprofloxacin (91% for C. jejuni and 86.1% for C. coli) and nalidixic acid (89.3% for C. jejuni and 85% for C. coli). Furthermore, resistance to two or more classes of antibiotics was found in the majority (60.9%) of Campylobacter spp. and among them one C. coli strain showed resistance to four different classes of antimicrobials. Identification of virulence genes in the isolated Campylobacter showed that all of them had the flaA and cadF genes. The iam marker was found more often in C. coli strains (88.8%) compared to C. jejuni isolates (53.8%). On the other hand, the virB11 gene was identified only in 4.2% of C. coli and in 6.1% of C. jejuni strains, respectively. Furthermore, the prevalence of the cdtA, cdtB, and cdtC genes among C. jejuni strains was 97.7%, 93.2%, 96.2%, respectively, and was significantly higher than for C. coli regarding the cdtC (66.7%) gene. The obtained results showed that the presence of Campylobacter in retail meat may represent a threat to public health.

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Section: Discussioncontrasting

confidence: 43%

“…These findings are in contrast to data obtained by other authors who in similar samples detected mostly C. jejuni (Sallam 2007;Rozynek et al 2008;Bardon et al 2011;Anonymous 2012a). However, there are also some reports were C. coli was more predominant than C. jejuni in poultry meat (Kurincic et al 2005;Lynch et al 2011;Mackiw et al 2012). …”

Section: Discussionmentioning

confidence: 99%

Prevalence, antimicrobial resistance, and molecular characterization of Campylobacter jejuni and C. coli isolated from retail raw meat in Poland

Wieczorek¹,

Szewczyk²,

Osek³

2012

Vet. Med.

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“…The disease characteristics vary from watery, nonbloody, non-inflammatory diarrhea to a severe inflammatory diarrhea followed by abdominal pain and fever [9]. Amongst all the species of Campylobacter, Campylobacter jejuni subsp jejuni, Campylobacter coli, and Campylobacter lari are the most prevalent pathogens in human [10,11], with C. jejuni being responsible for 80-90% and C. coli for 5-10% infections in human [12]. Campylobacter spp, especially C. jejuni subsp.…”

Section: Introductionmentioning

confidence: 99%

“…Fluoroquinolone use in food animals (administration in poultry flocks which is the main reservoir of Campylobacter spp.) may be associated with increases of resistant Campylobacter strains in human health care [12,17]. The latter has been well documented in several countries [12,18,19].…”

Section: Introductionmentioning

confidence: 99%

“…may be associated with increases of resistant Campylobacter strains in human health care [12,17]. The latter has been well documented in several countries [12,18,19]. Mechanism of fluoroquinolone resistance was found to be chromosomally mediated through mutation of the gyrA gene and parC gene while macrolides resistance in C. jejuni is through the 23S rRNA.…”

Section: Introductionmentioning

confidence: 99%

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Antibiotic resistance profiles of Campylobacter species in the South Africa private health care sector

Shobo

Bester

Baijnath

et al. 2016

J Infect Dev Ctries

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Introduction: There is a dearth of surveillance data on clinical Campylobacter in South Africa, particularly in the private healthcare environment. We investigated the prevalence of resistance to first-line antibiotics used to treat campylobacterioses in clinical Campylobacter isolates from a private pathology laboratory. Methodology: Identification of the Campylobacter specific genes were confirmed by PCR. Minimum inhibitory concentrations were determined using the broth micro-dilution method against macrolides (erythromycin, azithromycin), fluoroquinolones (ciprofloxacin, gatifloxacin) and tetracycline. Results: Seventy-two Campylobacter isolates were identified by PCR, with 54 (75%) being classified as C. jejuni and 18 (25%) as C. coli. Of these, 11 (20.4%) C. jejuni and six (33.3%) C. coli strains were resistant to ciprofloxacin and three (7.41%) C. jejuni and three (16.7%) C. coli strains were resistant to gatifloxacin. The number of C. jejuni strains resistant to erythromycin and azithromycin was 17 (31.5%) and 36 (50%) respectively, while the resistance of C. coli strains to erythromycin and azithromycin were seven (38.9%) and 14 (77.8%) respectively. Resistance to tetracycline was detected in 10 (55.6%) C. coli and 14 (25.9%) C. jejuni strains. Conclusion: In the light of these resistant profiles, the lack of a South African Campylobacter surveillance program is of concern. Relatively high prevalence of resistance in clinical isolates of C. jejuni and C. coli to the fluoroquinolones, macrolides and tetracycline used in first line treatment is of great concern. The efficacy treating human campylobacteriosis should thus be revisited.

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Antimicrobial Resistance ofCampylobactersp

Osaili

Alaboudi

2016

Foodborne Pathogens and Antibiotic Resistance

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Antibiotic resistance in Campylobacter jejuni and Campylobacter coli isolated from food in Poland

Cited by 59 publications

References 43 publications

Prevalence, antimicrobial resistance, and molecular characterization of Campylobacter jejuni and C. coli isolated from retail raw meat in Poland

Prevalence, antimicrobial resistance, and molecular characterization of Campylobacter jejuni and C. coli isolated from retail raw meat in Poland

Antibiotic resistance profiles of Campylobacter species in the South Africa private health care sector

Antimicrobial Resistance ofCampylobactersp

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