Antibiotic resistance in Enterobacter hormaechei

Yeh, Ting-Kuang; Lin, Hsueh‐Ju; Liu, Po‐Yu; Wang, Jui-Hsing; Hsueh, Po‐Ren

doi:10.1016/j.ijantimicag.2022.106650

Cited by 25 publications

(13 citation statements)

References 112 publications

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“…xiangfangensis isolates were identified in the Chinese study ( Liu et al, 2022 ). Since E. hormaechei was first identified in 1989 ( Ohara et al, 1989 ), it has been reported from natural environments, and has recently emerged worldwide as ECC nosocomial pathogen ( Yeh et al, 2022 ). Despite being the most frequently isolated species of Enterobacter in South Korea, as determined by our study, E. hormaechei has only occasionally been reported.…”

Section: Discussionmentioning

confidence: 99%

“…Although the ST171 clone was not identified in this study, it is necessary to monitor the spread of MBL-producing E. hormaechei subsp. xiangfangensis isolates ( Roberts et al, 2020 ; Yeh et al, 2022 ). It is also noteworthy that colistin resistance was identified infrequently among the four subspecies of E. hormaechei and E. ludwigii , whereas most isolates of E. kobei , E. asburiae , E. roggenkampii , and E. cloacae were resistant to colistin.…”

Section: Discussionmentioning

confidence: 99%

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Species identification, antibiotic resistance, and virulence in Enterobacter cloacae complex clinical isolates from South Korea

Ganbold

Seo

et al. 2023

Front. Microbiol.

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This study aimed to identify the species of Enterobacter cloacae complex (ECC) isolates and compare the genotype, antibiotic resistance, and virulence among them. A total of 183 ECC isolates were collected from patients in eight hospitals in South Korea. Based on partial sequences of hsp60 and phylogenetic analysis, all ECC isolates were identified as nine species and six subspecies. Enterobacter hormaechei was the predominant species (47.0%), followed by Enterobacter kobei, Enterobacter asburiae, Enterobacter ludiwigii, and Enterobacter roggenkampii. Multilocus sequence typing analysis revealed that dissemination was not limited to a few clones, but E. hormaechei subsp. xiangfangensis, E. hormaechei subsp. steigerwaltii, and E. ludwigii formed large clonal complexes. Antibiotic resistance rates were different between the ECC species. In particular, E. asburiae, E. kobei, E. roggenkampii, and E. cloacae isolates were highly resistant to colistin, whereas most E. hormaechei and E. ludwigii isolates were susceptible to colistin. Virulence was evaluated through serum bactericidal assay and the Galleria mellonella larvae infection model. Consistency in the results between the serum resistance and the G. mellonella larvae infection assay was observed. Serum bactericidal assay showed that E. hormaechei, E. kobei, and E. ludwigii were significantly more virulent than E. asburiae and E. roggenkampii. In this study, we identified the predominant ECC species in South Korea and observed the differences in antibiotic resistance and virulence between the species. Our findings suggest that correct species identification, as well as continuous monitoring is crucial in clinical settings.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Species identification, antibiotic resistance, and virulence in Enterobacter cloacae complex clinical isolates from South Korea

Ganbold

Seo

et al. 2023

Front. Microbiol.

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“…It is also frequently isolated from infant food products, including PIF, with recent information highlighting its resistance to antibiotics [ 28 , 29 ]. Clinical isolates of E. hormaechei with different degrees of beta-lactam resistance have also been increasingly reported, as well as with the production of extended-spectrum β-lactamases, AmpC β-lactamases, and carbapenemases worldwide [ 30 ]. Of the five E. hormaechei isolates in our study, three were resistant to cephalothin and two strains to ampicillin.…”

Section: Discussionmentioning

confidence: 99%

Are Enterobacteriaceae and Enterococcus Isolated from Powdered Infant Formula a Hazard for Infants? A Genomic Analysis

Parra‐Flores

Rosel

Daza-Prieto

et al. 2022

Foods

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Powdered infant formulas (PIF) are the most used dietary substitutes that are used in order to supplement breastfeeding. However, PIF are not sterile and can be contaminated with different microorganisms. The objective of this study was to genomically characterize Enterobacteriaceae (ENT) and Enterococcus strains that were isolated from PIF. Strains were identified by matrix-assisted laser desorption ionization–time-of-flight mass spectrometry (MALDI-TOF MS) and whole-genome sequencing (WGS). Genomic typing, detection of virulence, and resistance profiles and genes were performed with the Ridom SeqSphere+ software; the comprehensive antibiotic resistance database (CARD) platform; ResFinder and PlasmidFinder tools; and by the disk diffusion method. Nineteen isolates from PIF were analyzed, including ENT such as Kosakonia cowanii, Enterobacter hormaechei, Franconibacter helveticus, Mixta calida, and lactic acid bacteria such as Enterococcus faecium. The strains exhibited resistance to beta-lactams, cephalosporins, and macrolides. Resistance genes such as AcrAB-TolC, marA, msbA, knpEF, oqxAB, fosA, blaACT-7, blaACT-14, qacJ, oqxAB, aac(6’)-Ii, and msr(C); and virulence genes such as astA, cheB, cheR, ompA ompX, terC, ironA, acm, and efaAfm, adem were also detected. All the analyzed strains possessed genes that produced heat-shock proteins, such as IbpA and ClpL. In PIF, the presence of ENT and Enterococcus that are multiresistant to antibiotics—together with resistance and virulence genes—pose a health risk for infants consuming these food products.

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“…From the results, the MS2 was identi ed to be multi-drug resistant of high concern as it showed resistance to the major drugs classes like Amoxicillin/clavulanate (MIC-32), Nitrofurantoin (MIC-256), Cefuroxime axetil (MIC-64), trimethoprim-sulfamethoxazole (MIC-32), carbapenems (ertapenem and meropenem MIC-8), piperacillin/tazobactam (MIC-128), cefoperazone/sulbactam (MIC-64) and colistin (MIC-16). However, the Ms2 showed sensitive MIC values to amikacin (MIC-8) and tigecycline (MIC-0.5) [7].…”

Section: Bacterial Strain Identi Cation and Antibiotic Sensitivity Testmentioning

confidence: 99%

“…hormaechei in poultry farm settings to identify its silent epidemiological dynamics in human hosts and the environment [7].…”

Section: Introductionmentioning

confidence: 99%

Predicting human risk with multidrug resistant Enterobacter hormaechei MS2 with MCR 9 gene isolated from the feces of healthy broiler through whole genome sequence based analysis.

Sreejith

Premnath

PR³

et al. 2023

Preprint

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The zoonotic spread of antimicrobial resistance (AMR) and the associated infections are becoming a major threat to the human population worldwide. Strategies to identify the potential pathogen dissemination by seemingly healthy livestock are at a nascent stage and it is of significant importance to monitor environmental evolution of AMR. In this study, a multidrug resistant strain of Enterobacter hormaechei MS2 isolated from the feces of healthy broiler chicken has been characterized by whole genome sequencing based method. Here, the isolate was primarily subjected to antimicrobial susceptibility testing followed genome sequencing and analysis. From the antimicrobial susceptibility testing result, the strain was found to be resistant to multiple classes of drugs including the colistin which is an important candidate drug used to treat infectious diseases. The resistome prediction of genomic data further revealed the presence of 7 perfect and 26 strict hits including those for MCR-9 and FosA6. The pathogenicity prediction has also demonstrated the strain to have the potential to be a human pathogen with 0.72 probability. The phylogenetic analysis has also supported the zoonotic potential of the strain due to its clustering with isolates from both human and livestock-associated host groups. The results of the study suggest the need for a strong surveillance system to identify the opportunistic zoonotic pathogens to prevent a silent AMR menace mediated by them. Carriage of multi-drug resistant strains in the livestock gut microbiome is also a serious concern as it has high AMR transmissibility through contact and supply chain activities.

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Antibiotic resistance in Enterobacter hormaechei

Cited by 25 publications

References 112 publications

Species identification, antibiotic resistance, and virulence in Enterobacter cloacae complex clinical isolates from South Korea

Species identification, antibiotic resistance, and virulence in Enterobacter cloacae complex clinical isolates from South Korea

Are Enterobacteriaceae and Enterococcus Isolated from Powdered Infant Formula a Hazard for Infants? A Genomic Analysis

Predicting human risk with multidrug resistant Enterobacter hormaechei MS2 with MCR 9 gene isolated from the feces of healthy broiler through whole genome sequence based analysis.

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