Antibiotic resistance in Escherichia coli strains isolated from Antarctic bird feces, water from inside a wastewater treatment plant, and seawater samples collected in the Antarctic Treaty area

Rabbia, Virginia; Bello-Toledo, Helia; Jiménez, Sebastián; Quezada, M.; Domı́nguez, Mariana; Vergara, Luis Eduardo; Gómez-Fuentes, Claudio; Calisto-Ulloa, Nancy; González‐Acuña, Daniel; López, Juana; González-Rocha, Gerardo

doi:10.1016/j.polar.2016.04.002

Cited by 42 publications

(39 citation statements)

References 22 publications

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“…Moreover, previous studies detected multidrug-resistant bacteria recovered from penguin feces in Torgensen Island and in the Palmer Station (Anvers Island) 15 . In addition we have previously published a study reporting E. coli resistant to STR and TET isolated from an area of Fildes Bay close to military and scientific bases 14 . In addition, Antelo and Batista (2013) detected bacterial isolates collected in Antarctica with high levels of antibiotic resistance, including aminoglycosides, β-lactams and trimethoprim, which is consistent with our findings 29 .…”

Section: Discussionmentioning

confidence: 99%

Antibiotic resistance in bacterial isolates from freshwater samples in Fildes Peninsula, King George Island, Antarctica

Jara

Bello-Toledo

Domı́nguez

et al. 2020

Sci Rep

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Anthropic activity in Antarctica has been increasing considerably in recent years, which could have an important impact on the local microbiota affecting multiple features, including the bacterial resistome. As such, our study focused on determining the antibiotic-resistance patterns and antibioticresistance genes of bacteria recovered from freshwater samples collected in areas of Antarctica under different degrees of human influence. Aerobic heterotrophic bacteria were subjected to antibiotic susceptibility testing and pcR. the isolates collected from regions of high human intervention were resistant to several antibiotic groups, and were mainly associated with the presence of genes encoding aminoglycosides-modifying enzymes (AMes) and extended-spectrum β-lactamases (eSBLs). Moreover, these isolates were resistant to synthetic and semi-synthetic drugs, in contrast with those recovered from zones with low human intervention, which resulted highly susceptible to antibiotics. on the other hand, we observed that zone A, under human influence, presented a higher richness and diversity of antibiotic-resistance genes (ARGs) in comparison with zones B and c, which have low human activity. our results suggest that human activity has an impact on the local microbiota, in which strains recovered from zones under anthropic influence were considerably more resistant than those collected from remote regions.

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Section: Discussionmentioning

confidence: 99%

Antibiotic resistance in bacterial isolates from freshwater samples in Fildes Peninsula, King George Island, Antarctica

Jara

Bello-Toledo

Domı́nguez

et al. 2020

Sci Rep

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“…Bacteria, such as E. coli and K. pneumoniae , found in normal bowel flora, play a crucial role in the spread of resistance genes and can act as important reservoir for ESBL-type genes. In a study published in 2016, it is concluded that naturally occurring antibiotic resistance in E. coli strains from Antarctic bird is rare and the bacterial antibiotic resistance found in seawater is probably associated with discharged treated wastewater (60). …”

Section: Discussionmentioning

confidence: 99%

Anthropogenic antibiotic resistance genes mobilization to the polar regions

Hernández

González‐Acuña

2016

Infection Ecology & Epidemiology

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Anthropogenic influences in the southern polar region have been rare, but lately microorganisms associated with humans have reached Antarctica, possibly from military bases, fishing boats, scientific expeditions, and/or ship-borne tourism. Studies of seawater in areas of human intervention and proximal to fresh penguin feces revealed the presence of Escherichia coli strains least resistant to antibiotics in penguins, whereas E. coli from seawater elsewhere showed resistance to one or more of the following antibiotics: ampicillin, tetracycline, streptomycin, and trim-sulfa. In seawater samples, bacteria were found carrying extended-spectrum β-lactamase (ESBL)-type CTX-M genes in which multilocus sequencing typing (MLST) showed different sequence types (STs), previously reported in humans. In the Arctic, on the contrary, people have been present for a long time, and the presence of antibiotic resistance genes (ARGs) appears to be much more wide-spread than was previously reported. Studies of E coli from Arctic birds (Bering Strait) revealed reduced susceptibility to antibiotics, but one globally spreading clone of E. coli genotype O25b-ST131, carrying genes of ESBL-type CTX-M, was identified. In the few years between sample collections in the same area, differences in resistance pattern were observed, with E. coli from birds showing resistance to a maximum of five different antibiotics. Presence of resistance-type ESBLs (TEM, SHV, and CTX-M) in E. coli and Klebsiella pneumoniae was also confirmed by specified PCR methods. MLST revealed that those bacteria carried STs that connect them to previously described strains in humans. In conclusion, bacteria previously related to humans could be found in relatively pristine environments, and presently human-associated, antibiotic-resistant bacteria have reached a high global level of distribution that they are now found even in the polar regions.

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“…A phylogenetic dendrogram was constructed based on PFGE fingerprint profiles. Isolates sharing at least 80% similarity were considered genetically related, and those sharing 100% similarity were classified as clones (Balière, Rincé, Blanco, et al, 2015;Kao et al, 2016;Rabbia et al, 2016).…”

Section: Molecular Typingmentioning

confidence: 99%

“…Antimicrobial resistance (AMR) is another tool for characterization of bacterial isolates. With the wide use of antimicrobials in humans and in the environment, AMR E. coli have been reported from different sources and countries (Dou et al, 2016;Kao et al, 2016;Rabbia et al, 2016;Zhang et al, 2016). The presence of resistant organisms in the environment is an emerging concern around the world (Watkinson, Micalizzi, Graham, Bates, & Costanzo, 2007), and since AMR bacteria can accumulate in bivalve mollusks (Barkovskii, Green, & Hurley, 2010), it has been suggested that bivalves may be useful in assessing environmental contamination by AMR bacteria (Berendonk et al, 2015;Rees et al, 2015).…”

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Genotypic and phenotypic characterization of Escherichia coli isolated from mollusks in Brazil and the United States

et al. 2018

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The aim of this study was to determine the serogroups, antimicrobial resistance and genetic diversity of Escherichia coli isolates from samples of bivalve mollusks collected along Santa Catarina coast, Brazil, and from the Chesapeake Bay, Maryland, USA. One hundred forty‐one E. coli isolates were characterized for serogroups with 181 specific O antisera and antimicrobial susceptibility using the disk diffusion method. The genetic diversity was assessed using pulsed‐field gel electrophoresis (PFGE). The results showed that among the isolates, 19.9% were classified as multi‐drug resistant (MDR) and resistance was most frequently observed to cephalothin, nitrofurantoin, and ampicillin. The predominant serogroups were O6, O8, and O38. Some serogroups were recognized as pathogenic E. coli . PFGE dendrograms indicated extensive genetic diversity among the isolates. Although characteristics of the E. coli isolates were highly variable, it is important to note that E. coli belonging to pathogenic serogroups and MDR isolates are present in mollusks of both study areas. This is the first report on the phenotypic and genotypic characterization of E. coli from mollusks from Santa Catarina and the Chesapeake Bay that should encourage studies focusing on comparison of isolates across countries.

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Antibiotic resistance in Escherichia coli strains isolated from Antarctic bird feces, water from inside a wastewater treatment plant, and seawater samples collected in the Antarctic Treaty area

Cited by 42 publications

References 22 publications

Antibiotic resistance in bacterial isolates from freshwater samples in Fildes Peninsula, King George Island, Antarctica

Antibiotic resistance in bacterial isolates from freshwater samples in Fildes Peninsula, King George Island, Antarctica

Anthropogenic antibiotic resistance genes mobilization to the polar regions

Genotypic and phenotypic characterization of Escherichia coli isolated from mollusks in Brazil and the United States

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