Antibiotic resistance in Escherichia coli isolates obtained from animals, foods and humans in Spain

Sáenz, Yolanda; Zarazaga, Myriam; Briñas, Laura; Lantero, M; Ruiz-Larrea, Fernanda; Torres, Carmén

doi:10.1016/s0924-8579(01)00422-8

Cited by 156 publications

(111 citation statements)

References 21 publications

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“…Results of antimicrobial susceptibility testing (Table 2) showed high resistance to tetracycline, cephalothin, amoxicillin and streptomycin. These findings agree with data from previous studies showing that resistance is common among strains isolated from food, animals and meat (23,25).…”

Section: Discussionsupporting

confidence: 93%

Isolation of Shiga toxigenic Escherichia coli from butcheries in Taquaritinga city, State of São Paulo, Brazil

Rodolpho

Marin

2007

Braz. J. Microbiol.

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Shiga toxigenic Escherichia coli (STEC) has been implicated as the cause of several human diseases. Samples (ground beef, grinding-machines and human hands) from 23 butcheries were assayed for E. coli using standard microbiological methods, and 287 isolates were submitted to polymerase chain reaction for the detection of stx 1, stx 2 and eae genes. Four STEC isolates were recovered, two from ground beef and two from grinding-machines; all harbored the stx 2 gene and were negative for the eae gene. All E. coli isolates including the four STEC were screened for antibiotic resistance. High levels of resistance against several antimicrobial agents were detected; those most commonly observed were to tetracycline (76.6%), amoxicillin (64.1%) and cephalothin (58.8%). Such high levels of antimicrobial resistance highlight the need for a more rational use of these agents in cattle.

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Section: Discussionsupporting

confidence: 93%

Isolation of Shiga toxigenic Escherichia coli from butcheries in Taquaritinga city, State of São Paulo, Brazil

Rodolpho

Marin

2007

Braz. J. Microbiol.

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“…Ampicillin ( On the other hand, the resistance levels found for some antimicrobials in isolates obtained from the chicken meat tend to be higher than the levels reported in other countries (Bywater et al, 2004;Kijima-Tanaka et al, 2003;Miranda et al, 2008a;Sa´enz et al, 2001;Van den Bogaard et al, 2001). It is remarkable that the high level of resistance observed in poultry-origin isolates for both antimicrobials are commonly used in poultry medicine, such as ampicillin (76.5%), doxycycline (56.3%), sulfisoxazole (77.3%), or ciprofloxacin (77.3%) (Miranda et al, 2008b;Van den Boogard et al, 2001) and also for antimicrobials that are rarely used in poultry medicine, such as chloramphenicol (58%).…”

Section: Resultsmentioning

confidence: 92%

“…During recent years, in addition to official surveillance and antimicrobial monitoring programmes (CI-PARS, 2006;DANMAP, 2006;MARAN, 2005;NARMS, 2007;SVARM, 2005;VAV Network, 2005), some studies have reported the antimicrobial resistance of E. coli isolated from meat samples and food-producing animals (Bywater et al, 2004;Guerra et al, 2003;Kijima-Tanaka et al, 2003;Knezevic & Petrovic, 2008;Miranda et al, 2008a;Sa´enz et al, 2001; Van den Bogaard et al, 2001). However, these works involved samples taken directly from live or recently slaughtered animals, and do not take into consideration the contamination of foods during subsequent steps in the food chain.…”

Section: Resultsmentioning

confidence: 99%

“…When compared with the other microorganisms found mostly in foods, Escherichia coli has been a very useful biomarker for evaluating the development of antimicrobial resistance (Von Baum & Marre, 2005). In addition to its high frequency of mutation, which can favour the spontaneous development of antimicrobial resistance, E. coli also has the ability to transfer microbial resistance between individual bacteria (Kijima-Tanaka et al, 2003;Sa´enz et al, 2001;Sayah, Kaneene, Johnson, & Miller, 2005;Von Baum & Marre, 2005). Thus, changes in the resistance of this species may serve as a good indicator of resistance in potentially pathogenic bacteria (Kijima-Tanaka et al, 2003;Von Baum & Marre, 2005).…”

Section: Introductionmentioning

confidence: 99%

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Presence and antimicrobial resistance ofEscherichia coliisolated from foodstuffs in Hidalgo State (Mexico) Presencia y resistencia a antimicrobianos deEscherichia coliaislados a partir de alimentos en el estado de Hidalgo (México)

Miranda

Mondragón

Rodríguez

et al. 2010

CyTA - Journal of Food

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The presence of Escherichia coli in foods taken from the grocery stores and the supermarkets in Hidalgo State (Mexico) was determined for 73 samples of poultry meat, 60 samples of pork, 86 samples of beef, and 66 samples of vegetables. A total of 352 E. coli strains were isolated, identified, and analyzed by an agar disk diffusion assay for their resistance to 10 antimicrobials. Poultry meat and vegetables taken from groceries showed significantly higher counts (P ¼ 0.0002 and P ¼ 0.0461, respectively) when compared with the samples taken from supermarkets. Compared with the isolates recovered from other foods, E. coli isolated from chicken meat had higher levels of antimicrobial resistance against all antimicrobials tested, with the exceptions of nitrofurantoin resistance of isolates from pork and streptomycin resistance in isolates from pork and beef. In addition, the E. coli isolates from samples taken from the groceries showed higher resistance rates than the isolates from samples taken from the supermarkets for the cases of pork isolates resistance to ampicillin (P ¼ 0.0497), chloramphenicol (P ¼ 0.0075), doxycycline (P ¼ 0.002), and streptomycin (P ¼ 0.0094) and beef isolates resistance against ampicillin (P ¼ 0.0048), streptomycin (P ¼ 0.002), and sulfisoxazole (P ¼ 0.003). The present study revealed that the observed resistance rates correlated well with those reported in the national surveillance programmes of developed countries, with the exception of isolates from chicken meat, which have higher resistance rates. Also, from a microbiological safety point of view, samples taken from supermarkets were in a much better conditions than those obtained from the groceries.Keywords: E. coli; antimicrobial; food-borne; resistance; Me´xico La presencia de Escherichia coli fue investigada en 73 muestras de carne de pollo, 60 muestras de carne de cerdo, 86 muestras de carne de vacuno y 66 muestras de alimentos vegetales muestreados en pequen˜os ultramarinos y supermercados en el estado de Hidalgo (Me´xico). Un total de 352 aislamientos de E. coli fueron seleccionados, identificados y analizados mediante el me´todo de difusio´n en disco para determinar su resistencia a 10 antimicrobianos. Las muestras de pollo y vegetales obtenidas en tiendas de alimentacio´n mostraron recuentos de E. coli significativamente mayores (P ¼ 0,0002 y P ¼ 0,0461 respectivamente) que las obtenidas en supermercados. Comparados con los procedentes de los restantes alimentos, los E. coli obtenidos a partir de carne de pollo mostraron un mayor grado de resistencia a todos los antimicrobianos estudiados, excepto en lo referente a la nitrofurantoı´na en el caso de los aislados a partir de carne de cerdo y la estreptomicina respecto de los aislamientos de carne de cerdo y vacuno. Adicionalmente, en algunos casos, los E. coli obtenidos a partir de alimentos muestreados en pequen˜os ultramarinos mostraron mayores tasas de resistencia que los procedentes de alimentos muestreados en supermercados. Esto ocurrio´en los aislamientos procedentes de ca...

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“…High tetracycline resistance (39.5% -56.8%) in E. coli isolates sourced from chickens has been documented in Australia Barton and Wilkins, 2001;Obeng et al, 2012) as well as, globally (Hasan et al, 2011;Saenz et al, 2001). Obeng et al (2012) …”

Section: Tetracyclinementioning

confidence: 99%

Studies on avian pathogenic Escherichia coli in commercial broiler chicken in South East Queensland

Awawdeh¹,

Turni²,

Henning³

et al.

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Avian pathogenic Escherichia coli (APEC) is the causative agent of avian colibacillosis, a localised or systemic infection resulting in clinical diseases such as colisepticemia, chronic respiratory disease and swollen-head syndrome. Globally, avian colibacillosis is the leading cause of morbidity and mortality in poultry, and it has been associated with massive economic losses and welfare problems.This organism is of public health significance as APEC is communicable to humans. The diagnosis of avian colibacillosis relies on clinical signs, typical pathological lesions and culture of E. coli from affected tissue(s). Antimicrobial therapy is often used both for treatment and control. Previous overseas studies have characterised APEC and identified virulence genes (VGs) that can be used as molecular markers for the identification of APEC. Little is known about APEC in broiler chickens in Australia.The aim of this thesis was to gain a better understanding of the epidemiology of APEC in Australian broiler flocks and how factors including presence of VGs and phylogenetic group can improve the identification of this pathotype in Australia. Firstly, three faecal DNA extraction methods were evaluated. Faeces were collected from healthy chickens and chickens with colibacillosis from commercial broiler farms in South East Queensland (SEQ). The extracted DNA was screened by a pentaplex-PCR for five APEC-associated VGs (iroN, iutA, iss, hlyF and ompT). DNA extracted from E. coli isolates cultured from the cloaca and organs of the birds were screened using the same PCR.Repeated bead beating plus column elution was the preferred DNA extraction method, as it yielded good PCR quality and adequate quantity DNA. However, identifying APEC by direct detection of the five VGs from the faecal material was not feasible as all of these genes were also detected in all of the birds. However, the VGs were more commonly detected in E. coli isolates cultured from birds with colibacillosis.A cross-sectional study was performed to estimate APEC farm-level prevalence in healthy broiler chickens in SEQ and to identify potential risk factors associated with the carriage of APEC. At the farm-level, all of the 40 farms sampled were positive for APEC, that is at least one bird per farm carried APEC, while the within-farm prevalence was 63% (95% Confidence Interval: 55.8, 70.2).Higher APEC within-farm bird-level prevalence was significantly associated with the usage of well water as a source of drinking water, failure to disinfect the water line after each flock, farm visitors not showering before entering the shed, distances greater than 20 metres between the car park and the poultry shed and the presence of wild birds within 50 metres of the shed. Chlorinating the drinking water combined with automatic water filtration reduced within-farm bird-level APEC prevalence. Page | 3Therefore, based on the results concluded from the multivariable model, improving biosecurity and water treatments might reduce APEC prevalence, decrease the risk of co...

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Antibiotic resistance in Escherichia coli isolates obtained from animals, foods and humans in Spain

Cited by 156 publications

References 21 publications

Isolation of Shiga toxigenic Escherichia coli from butcheries in Taquaritinga city, State of São Paulo, Brazil

Isolation of Shiga toxigenic Escherichia coli from butcheries in Taquaritinga city, State of São Paulo, Brazil

Presence and antimicrobial resistance ofEscherichia coliisolated from foodstuffs in Hidalgo State (Mexico) Presencia y resistencia a antimicrobianos deEscherichia coliaislados a partir de alimentos en el estado de Hidalgo (México)

Studies on avian pathogenic Escherichia coli in commercial broiler chicken in South East Queensland

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