Antibiotic resistance in Escherichia coli isolated from healthy cattle at a major cattle market in Ibadan, Oyo State, South Western, Nigeria

Ogunleye, Ayodele A; Okunlade, A O; Jeminlehin, FO; Ajuwape, A.T.P.

doi:10.5897/ajmr2013.6028

Cited by 16 publications

(9 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Our results corroborated with the findings of Ogunleye et al (2013), but much lower than the findings of Masud et al (2012). Differences in geographical locations might be the reason for this as Islam et al (2014) reported a geographical variation in the prevalence of E. coli in cattle.…”

Section: Discussionsupporting

confidence: 92%

Prevalence and antibiotic susceptibility pattern of Escherichia coli in cattle on Bathan and intensive rearing system

et al. 2017

View full text Add to dashboard Cite

The aim of this longitudinal study was to verify the prevalence and antibiotic susceptibility pattern of Escherichia coli (E. coli) in cattle reared on Bathan and intensive farming system in Bangladesh. Fecal materials originated from recto anal junction (RAJ) of 100 cattle used for primary screening on MacConkey agar. The diversities among the pink color colony producing isolates on MacConkey agar were verified by conventional cultural methods and biochemical tests. Phenotypically positive E. coli isolates were further investigated for the variations in the antimicrobial resistance profiles to 10 selected antibiotics, by the disk-diffusion method. This study revealed that the overall prevalence of E. coli was 70% of in the rectal swab sample of cattle. However, the prevalence of E. coli was found significantly higher (p= 0.002) in cattle under intensive farming (84%) than cattle on Bathan (56%). Antibiotic susceptibility pattern shows that among the tested isolates 83%, 73%, 68% and 64% were sensitive to chloramphenicol, gentamicin, ciprofloxacin and ampicillin, respectively. On the other hand, all the 70 (100%) E. coli isolates were found resistant to tetracycline and sulfamethoxazole. A high antibiotic resistance profile was also found against amoxicillin (90%), ampicillin (87%), nalidixic acid (86%) and erythromycin (83%). In total, 24 (34%) isolates were resistant against ≥2 antimicrobials. The result clearly shows that antibiotic resistant E. coli isolates are commonly present in cattle of different management systems (intensive and Bathan). Therefore, careful selection of appropriate antibiotics with optimal doses might be ensured to prevent the emergence of antibiotic resistance bacteria.

show abstract

Section: Discussionsupporting

confidence: 92%

Prevalence and antibiotic susceptibility pattern of Escherichia coli in cattle on Bathan and intensive rearing system

et al. 2017

View full text Add to dashboard Cite

show abstract

“…According to this study prevalence of E. coli was high in cattle ≥3 years of age, female and unorganized farming systems, but the differences were not significant. The overall prevalence as obtained in this study support the findings of Ogunleye et al (2013), who described a prevalence of 80% in the apparently healthy cattle of Nigeria but higher than the prevalence described by the Masud et al (2012). According to Masud et al (2012), the prevalence of E. coli in the rectal swab of apparently healthy cattle of 2-3 years of age is 23.21% in another geographic location in Bangladesh.…”

Section: Discussionsupporting

confidence: 90%

“…These differences might be due to the differences in methodology employed in these studies. Ogunleye et al (2013) and Masud et al (2012) described their findings based on cultural and biochemical examination. In this study, in addition to traditional techniques i.e., morphology, staining, cultural and biochemical test, PCR was employed for the confirmatory identification of E. coli from rectal swab of apparently health cattle.…”

Section: Discussionmentioning

confidence: 99%

Prevalence and Characterization of Escherichia coli from Rectal Swab of Apparently Healthy Cattle in Mymensingh, Bangladesh

et al. 2014

View full text Add to dashboard Cite

Cattle are considered as one of the sources of pathogenic E. coli worldwide. The present study was designed to determine the prevalence and identification of the E. coli isolated from rectal swab of apparently healthy cattle in Mymensingh, Bangladesh. A total of 128 rectal swab samples were assessed by cultural, morphological and biochemical examination followed by Polymerase Chain Reaction (PCR) using primers ECO-1 and ECO-2 that are specific for E. coli 16S rRNA gene. Data obtained from this study were analyzed based on the age, sex, breed and management systems of cattle. This study revealed a 75% prevalence of E. coli in the rectal swab of cattle. Higher prevalence was found in female cattle of unorganized farming systems, and in cattle ≥3 years of age. From this study, it may be concluded that, irrespective of age, sex, breed and management system, E. coli is prevailing in the rectal swab of apparently healthy cattle.

show abstract

“…In particular, most of isolated bacteria was E. coli at the proportion of 85% (72 sample) of total sample and the rest of samples were contained the isolates of other coliforms. Similar results have also been obtained from the study in Nigeria and Bangladesh, both reported the prevalence of E. coli from sample of rectal swab of cattle between 75%-80% [17,18]. Interestingly, both of studies were taken sample from the cattle those assumed healthy.…”

Section: Isolation Of E Colisupporting

confidence: 84%

Isolation, Identification, and Critical Points of Risk of Escherichia coli O157:H7 Contamination at Aceh Cattle Breeding Centre

et al. 2020

View full text Add to dashboard Cite

This study was aimed to identify possible critical points of Escherichia coli (E. coli ) O157:H7, a pathogenic agent, contamination in aceh cattle breeding centre. For this purpose, samples were collected from cattle faeces, hand of workers (animal keepers), and water sources in the farm using cross-sectional approach. A number of 85 samples of cattle faecal swab were collected randomly from the animals in the breeding centre. The samples of swab of hand of all workers (15 persons) were collected before and after work. Then, the water sources from 11 cattle house locations in the breeding centre were collected. The water sources were divided into three different locations, namely the water containers, taps, and water puddle on the floors. At each source a number of 11 samples were collected. Isolation of E. coli was conducted on Eosin Methylene Blue Agar (EMBA), followed by identification on Sorbitol MacConkey Agar (SMAC). Then Molecular subtyping of E. coli O157:H7 genes was conducted using multiplex-PCR analysis. Data were analysed descriptively. The results of this study showed that 72 samples (85 %) among 85 samples were positive for E. coli and the rest of samples were positive for other types of bacteria. Sample isolation from swabs of hand was found 3 positive E. coli before work and 1 positive E. coli after work from 15 workers. The most potential water sources for E. coli contamination were the water in taps, and water puddle on the floor of cattle houses. Then, two of samples of E. coli isolated from rectal swab were confirmed as E. coli O157:H7 using PCR test, based on the presence of stx2 gene. In conclusion, the risk of presence of E. coli as zoonotic agents of E. coli O157:H7 in aceh cattle as well as from the farm workers and surrounding area are high. An appropriate control strategy is needed to apply in the aceh cattle farm to prevent from E. coli O157:H7 outbreak in the future.

show abstract

Antibiotic resistance in Escherichia coli isolated from healthy cattle at a major cattle market in Ibadan, Oyo State, South Western, Nigeria

Cited by 16 publications

References 13 publications

Prevalence and antibiotic susceptibility pattern of Escherichia coli in cattle on Bathan and intensive rearing system

Prevalence and antibiotic susceptibility pattern of Escherichia coli in cattle on Bathan and intensive rearing system

Prevalence and Characterization of Escherichia coli from Rectal Swab of Apparently Healthy Cattle in Mymensingh, Bangladesh

Isolation, Identification, and Critical Points of Risk of Escherichia coli O157:H7 Contamination at Aceh Cattle Breeding Centre

Contact Info

Product

Resources

About