Antibiotic resistance in Escherichia coli isolates from poultry environment and UTI patients in Kerala, India: A comparison study

Sebastian, Stelvin; Tom, Antriya Annie; Babu, Joyal Anna; Joshy, Merin

doi:10.1016/j.cimid.2021.101614

Cited by 16 publications

(7 citation statements)

References 13 publications

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“…A recent study conducted in North India observed a high level of resistance genes against fluoroquinolones followed by tetracycline and beta-lactams [72]. Recent studies conducted in Kerala found that antibiotics were supplied along with feed, leading to antibiotic resistance [73]. Similarly, the study found E. coli resistance to beta-lactams and carbapenem in the poultry litter, and similar resistance has been observed and correlated with isolation from patients with UTI in that area.…”

Section: Antibiotic Use In Poultrymentioning

confidence: 53%

Antimicrobial Resistance: A One Health Perspective in India

Rahul,

Damodharan,

Vajravelu Leela

et al. 2024

Antimicrobial Stewardship - New Insights

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One health is a collaborative, multi-sectoral, trans-disciplinary approach used to achieve optimal health and well-being outcomes that recognize the interconnections among people, animals, plants, and their shared environment. This approach is crucial because animals and people are colonized by the same bacteria species and treated with the same antibiotic classes; the technique is instrumental in fighting antibiotic resistance. The microorganism developed antibiotic-resistant genes, which were transferred to the animal and human population via the environment. Human activities speed up the organism to acquire resistance rapidly. The primary sources of antimicrobial resistance from the environment were improper sewage and hospital waste sanitation, effluents from antibiotic production units, animal husbandry waste, agricultural manure use, livestock, and aquatic sources. This study analyzed the various routes by which antimicrobial-resistant gene is transferred into humans and their pathway in India. The study concludes that implementing strict regulation and monitoring regarding the irrational use of antibiotics in animals, sewage disposal, waste disposal, and hospital infection control practices, and providing awareness to the public regarding antibiotic resistance can reduce the rate of developing antibiotic resistance to some extent along with implementing antibiotic stewardship programmes for veterinary medicine.

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Section: Antibiotic Use In Poultrymentioning

confidence: 53%

Antimicrobial Resistance: A One Health Perspective in India

Rahul,

Damodharan,

Vajravelu Leela

et al. 2024

Antimicrobial Stewardship - New Insights

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“…The feces were the source of the dissemination of multidrugresistant E. coli from poultry to the human population (Sebastian et al, 2021;Day et al, 2019). Therefore, the present study aims to identify the genotypic resistance pattern of ESBL E. coli from poultry fecal samples by screening ESBL genes (bla TEM , bla SHV , bla OXA , and bla CTX-M group 1, 2, and 9).…”

Section: Introductionmentioning

confidence: 99%

Whole genome sequencing and characteristics of extended-spectrum beta-lactamase producing Escherichia coli isolated from poultry farms in Banaskantha, India

Patel¹,

Pandey

Patel

et al. 2022

Front. Microbiol.

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Worldwide dissemination of extended-spectrum -lactamase (ESBL)-producing Escherichia coli constitutes an emerging global health issue, with animal food products contributing as potential reservoirs. ESBL E. coli infection is associated with the high mortality and mobility rate in developing countries due to less susceptibility to antibiotics. The present study aimed to elucidate the molecular characteristics and sequence-based analysis of ESBL E. coli in the Gujarat state of India. This study included 108 E. coli strains were isolated from different poultry farms (broiler and layer) in the Banaskantha District. PCR was employed to identify genotypic ESBL-producing antimicrobial resistance genes. Overall, a high occurrence of ESBL genes was found in poultry farms due to the high usage of antimicrobials. The PCR analysis revealed that 79.62% of isolates were detected positive with one or more ESBL genes. Among them, blaTEM (63.88%) was found to be the predominant genotype, followed by blaSHV (30.55%) and blaOXA (28.70%). In the blaCTX-M group, a higher occurrence was observed in blaCTX-M-9 (23.14%), followed by blaCTX-M-2 (24.07%) and blaCTX-M-1 (22.22%). We used the whole-genome sequencing (WGS) method to evaluate the antimicrobial resistance genes, virulence factors, single nucleotide polymorphisms (SNPs), plasmid replicons, and plasmid-mediated AMR genes of one ESBL E. coli isolated. We examined the genetic relatedness of a human pathogenic E. coli strain by comparing its sequence with the broad geographical reference E. coli sequences. Escherichia coli ST 681 was determined using multi-locus sequence typing. We compared our findings to the reference sequence of Escherichia coli str. K- 12 substr. MG1655. We found 24,937 SNPs with 21,792 in the genic region, 3,145 in the intergenic region, and six InDels across the genome. The WGS analysis revealed 46 antimicrobial resistance genes and seven plasmid-mediated AMR genes viz., tetA, qnrS1, dfrA14, sul2, aph(3”)-lb, aph(6)-ld, and Aph(3’)-la. The ST 681 was found to have Cib, traT, and terC virulence factors and two plasmid replicons, IncFII(pHN7A8) and IncI1-I(Alpha). This study revealed a higher occurrence of ESBL E. coli detected in poultry.

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“…Some antibiotics commonly used in philippines such as tetracycline, amoxicillin, erythromycin, gentamicin (Barroga et al, 2020) together with other feed additives are also used to increase the production performance of layer pullets (The Philippines Recommends for Table Egg Production, 2008). However, the use of antibiotics raises an issue concerning the development of antibiotic resistance of some pathogenic bacteria such as Escherichia coli (Sebastian et al, 2021); Listeria monocytogenes (Panera-Martinez et al, 2022); and Typhoidal Salmonella (Yin et al, 2021). Due to this occurrence, farm owners have an emerging trend in using a more natural way of giving their pullets protection against pathogenic bacteria.…”

Section: Introductionmentioning

confidence: 99%

The impact of avian IgY antibodies and probiotics supplementation via drinking water on growth and laying performance of 16-week-old layer pullets

Medina

Purnamasari

Olarve³

et al. 2022

Aceh J. Anim. Sci.

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Antibiotics raise an issue concerning the development of antibiotic resistance of some pathogenic bacteria. The use of probiotics and Avian IgY in layer pullet farming is a better way of protecting against pathogenic bacteria and increasing production performance. Supplementation of Avian IgY antibodies and probiotics(Lactobacillus acidophilus, Streptococcus faecium, Bifido bacterium longghum, Bacillus thermophilum) were conducted on 5,724 healthy layer pullets to determine its effect on their growth and laying performance. The supplement was in powder form with a dosage of 100 mg per liter of water for 6 hours. The effect of the supplementation was evaluated by measuring production parameters on growth and laying performance of the layer pullets; there were 3 treatment groups, the control group, Treatment 2 which received 5 days initial supplementation for the first week and then twice every week for 9 weeks and Treatment 3 which received 5 days initial supplementation for the first week and then twice every 2 weeks for 9 weeks. Results showed that the supplementation had significant effects in the age in days when 50% of the total population per treatment laid their first eggs, average number of eggs produced, % Hen day production, % Hen house production, total weight of eggs, and feed conversion efficiency. It also showed that the supplementation is economical and yielded higher profit. On the other hand, the supplementation had no significant effect on the average body weight and mortality count of the layer pullets. The supplementation of Avian IgY antibodies and probiotics in water soluble powder via drinking water with 5 days initial supplementation of Avian IgY antibodies and probiotics for the first week; then twice every 2 weeks for 9 weeks has the greatest effects to increase the production performance and profitability of layers.Keywords: Avian IgY antibodies, Growth performance, Laying performance, Layer pullets, Probiotics

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Antibiotic resistance in Escherichia coli isolates from poultry environment and UTI patients in Kerala, India: A comparison study

Cited by 16 publications

References 13 publications

Antimicrobial Resistance: A One Health Perspective in India

Antimicrobial Resistance: A One Health Perspective in India

Whole genome sequencing and characteristics of extended-spectrum beta-lactamase producing Escherichia coli isolated from poultry farms in Banaskantha, India

The impact of avian IgY antibodies and probiotics supplementation via drinking water on growth and laying performance of 16-week-old layer pullets

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