Antibiotic resistance in foodborne listeria

Facinelli, Bruna; Giovanetti, Eleonora; Varaldo, Pietro E.; Casolari, P.; Fabio, U

doi:10.1016/0140-6736(91)92138-r

Cited by 45 publications

(30 citation statements)

References 7 publications

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“…To overcome these problems, a wide range of synthetic antimicrobial agents (sodium benzoate, calcium benzoate, sorbate) has been used as food preservatives (Sherwin, 1990). However, antibiotic resistance has been described among Listeria spp., particularly L. monocytogenes strains isolated from food, the environment, or sporadic human listeriosis cases (Poyart-Salmeron, et al, 1990;Facinelli et al, 1991;Charpentier et al, 1995).…”

Section: Introductionmentioning

confidence: 99%

Synergistic Antibacterial Activity of Ecklonia cava (Phaeophyceae: Laminariales) against Listeria monocytogenes (Bacillales: Listeriaceae)

Nshimiyumukiza¹,

Kang²,

Kim³

et al. 2015

Fisheries and aquatic sciences

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In an effort to discover alternative antimicrobials against Listeria monocytogenes, several marine algae were screened. The methanolic extract of Ecklonia cava exhibited the highest antibacterial activity against L. monocytogenes, with the ethyl acetate (EtOAc) soluble fraction of E. cava methanolic extract having a MIC value of 256 µg/mL and a MBC value of 512 µg/mL. The MIC values of streptomycin in combination with the EtOAc fraction were markedly reduced up to 64-fold, suggesting that the antibacterial activity of the antibiotic was restored when combined with the EtOAc fraction. The interaction between streptomycin and the EtOAc fraction was assessed by fractional inhibitory concentration (FIC) indices. The combination of streptomycin and the EtOAc fraction against L. monocytogenes resulted in ∑FIC min range of 0.141 to 0.266 and ∑FIC max of 0.531 for all strains. The median ∑FIC against L. monocytogenes strains ranged from 0.172 to 0.344. Thus, synergistic ranges of FIC <1 were observed for all combinations of streptomycin and the EtOAc fraction against L. monocytogenes strains. Indeed, the median ∑FIC of streptomycin-EtOAc fraction ranged from 0.172 to 0.344, suggesting a marked synergy.

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Section: Introductionmentioning

confidence: 99%

Synergistic Antibacterial Activity of Ecklonia cava (Phaeophyceae: Laminariales) against Listeria monocytogenes (Bacillales: Listeriaceae)

Nshimiyumukiza¹,

Kang²,

Kim³

et al. 2015

Fisheries and aquatic sciences

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“…Therefore, Caco-2 cells were chosen to study the virulence of L. monocytogenes strains exhibiting different lectin agglutination profiles. Only four of the five lectins of the original set (Facinelli et al, 1994) were used in the present study, as ConA appeared to be less specific in agglutinating listeriae. The results point to the involvement of surface carbohydrates in the virulence of L. monocytogenes.…”

Section: Introductionmentioning

confidence: 99%

“…In particular, multilocus enzyme analysis has demonstrated that despite the large number of electrophoretic types found among L. monocytogenes isolates, only a few are responsible for the majority of cases of human listeriosis (Piffaretti et al, 1989;Bibb et al, 1990;Norrung & Skovgaard, 1993 (Hof & Rocourt, 1992). We have recently used lectins -a class of specific sugarbinding proteins which recognize terminal carbohydrate residues on cell surfaces (Sharon & Lis, 1989) -to develop a microtitre assay by which the agglutination profiles of Listeria isolates can be determined and compared (Facinelli et al, 1994). Important differences emerged in the lectin agglutination profiles of L. monocytogenes strains : overall, food and environmental strains appeared to be more heterogeneous than clinical strains in their interaction with lectins, probably due to a greater variability in their surface receptors.…”

Section: Introductionmentioning

confidence: 99%

Lectin reactivity and virulence among strains of Listeria monocytogenes determined in vitro using the enterocyte-like cell line Caco-2

et al. 1998

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Forty-six cultures of Listeria monocytogenes (including clinical, food and collection strains) were serotyped, characterized for motility, haemolysis and phospholipase activities and tested for lectin agglutination using a four-lectin set. Lectin reactivity (i.e. agglutination by one or more of the four lectins) was observed in all 12 clinical isolates, 16 of the 23 food isolates and eight of the 11 collection strains. Virulence was evaluated in witro based on strains' ability to invade the human enterocyte-like cell line Caco-2. In gentamicin survival experiments, recovery of viable intracellular bacteria among lectin-unreactive strains was usually 1 0~1 0 0 0 -f o l d lower than among lectin-reactive haemolytic strains, and lower than among nonhaemolytic strains. Considerable cytopathogenic effects were produced by lectin-reactive haemolytic strains in trypan-blue-stained cell monolayers, whereas lectin-unreactive and nonhaemolytic strains produced no detectable cytopathogenic effect. Among lectin-reactive strains, the number of listerial cells associated with Caco-2 monolayers was more than tenfold greater than among lectin-unreactive strains. Cell invasion was inhibited by pretreatment of Caco-2 cells with sugars recognized by the lectins or of listeriae with enzymes which removed the same sugars from the bacterial surface. The results suggest that the study of lectin interactions could be helpful in understanding the pathogenicity potential of isolates of food and environmental origin.

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“…monocytogenes to exhibit antibiotic resistance and to harbor plasmids (4,39,48,69,70,167,184,189). Taken together, such findings suggest the potential for horizontal gene transfer among listeriae, with non-pathogenic strains serving as reservoirs for resistance determinants, potentially altering the fitness of the pathogenic species, L. monocytogenes.…”

Section: Identification Of Lmonocytogenes Antibiotic Resistance Genementioning

confidence: 69%

“…and L. welshimeri have been found to be more common than L. monocytogenes in food processing environments and in foods, and to grow faster than L. monocytogenes in foods and other media (8,47,104,140,168,197,201,221). They are also more likely than L.monocytogenes to exhibit antibiotic resistance and to harbor plasmids (4,39,48,69,70,167,184,189). Taken together, such findings suggest the potential for horizontal gene transfer 11 among listeriae, with non-pathogenic strains serving as reservoirs for resistance determinants, potentially altering the fitness of the pathogenic species, L. monocytogenes.…”

mentioning

confidence: 99%

Identification of the mechanisms of resistance and potential cross resistance of Listeria monocytogenes upon adaptation to different antimicrobials

Rakić-Martinez¹

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ACKNOWLEDGMENTSI would like to thank Dr. Vera Katić for being my mentor, not only through PhD studies, but in my undergraduate years as well. Her objectivity, articulate thinking and dedication to work inspired me to keep moving forward even after long, tiresome days in the lab. I will forever appreciate Dr. Katic's encouragement to pursue personal happiness and her support in important life decisions.I am forever grateful to Dr. Sophia Kathariou for accepting me in her lab and making me feel at home while far from it. Her incredible ability to find the best solutions when things were not looking promising and open completely new horizons along with her creativity and knowledge made science so attractive. Looking at all the great, bright and skilful young people pursuing their dreams under Dr. Kathariou's guidance makes me very proud for being a part of it.My appreciation also goes to Dr. Lepšanović and Dr. Mišić for accepting to be members of the committee. I am thankful to all my lab mates and to Ms. Robin Siletzky for her solid support throughout my work. A special thanks to Dr. Vikrant Dutta for his patience, willingness to help and his much admired teaching skills.Lastly and foremost I would like to thank my family. To my dear parents Ljubo and Zora whose unconditional love, support and presence whenever I needed them have been and will always be my motive to be better. To my big brother Zoran, who has been, and is my role model, but still owes me German lessons. Finally, I want to thank my boys, my beloved husband Sergio and my precious son Gregory, who have been an endless source of energy, support and love. Thanks for being my wingmen guys! ABSTRACTAbility to contaminate ready-to-eat, highly processed, cold-stored food products, relatively high fatality rate and environmental adaptations define .Listeria monocytogenes as a challenging foodborne pathogen of high public health significance. Listeria's adaptations and prolonged persistence in food processing facilities are a key link to contamination of foods.In recent years, bacterial multidrug resistance has been recognized as an emerging health challenge. Exposure to antimicrobials (antibiotics, disinfectants or environmentally ubiquitous heavy metals), may provide selective pressures for resistance with accompanying cross-resistance and increase in environmental fitness.In this work I addressed some of the possible mechanisms of adaptations that may contribute to persistence of L. monocytogenes in the environment as well as to survival and growth in different foods. This includes adaptations to heavy metals such as cadmium, disinfectants such as benzalkonium chloride (BC), selected antibiotics (e.g. the fluoroquinolone ciprofloxacin) and other toxic compounds (e.g. tetraphenyl-phosponium, often used as an anti-carcinogenic and efflux pump substrate). In order to characterize the impact of multidrug resistance on survival and growth of L. monocytogenes in different foods, we tested mutants adapted to higher concentrations of antibiotic ciprofloxacin and ...

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Antibiotic resistance in foodborne listeria

Cited by 45 publications

References 7 publications

Synergistic Antibacterial Activity of Ecklonia cava (Phaeophyceae: Laminariales) against Listeria monocytogenes (Bacillales: Listeriaceae)

Synergistic Antibacterial Activity of Ecklonia cava (Phaeophyceae: Laminariales) against Listeria monocytogenes (Bacillales: Listeriaceae)

Lectin reactivity and virulence among strains of Listeria monocytogenes determined in vitro using the enterocyte-like cell line Caco-2

Identification of the mechanisms of resistance and potential cross resistance of Listeria monocytogenes upon adaptation to different antimicrobials

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