Antibiotic Resistance in<i>Staphylococcus aureus</i>and Coagulase Negative Staphylococci Isolated from Goats with Subclinical Mastitis

Virdis, S.; Scarano, Christian; Cossu, F; Spanu, Vincenzo; Spanu, Carlo; Santis, E.P.L. De

doi:10.4061/2010/517060

Cited by 110 publications

(112 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Except for enroϐloxain and norϐloxacin, the present results indicate lower resistance rates to streptomycin, tetracycline and amoxicillin in Staphylococcus aureus as compared to CNS (Table 2). However, the multidrug resistant rate indicated here is higher than that (8%) recently reported in a study on antimicrobial resistance in coagulase positive and negative staphylococci from sub-clinical mastitis in goats (Virdis et al 2010).…”

Section: Discussioncontrasting

confidence: 47%

“…This rate of resistance to amoxicillin was higher than that described in S. aureus from mastitis in Brazilian dairy goats (Aires-de-Sousa et al 2007) but very similar to beta-lactam resistance rates reported worldwide for Staphylococcus aureus from cattle mastitis (Rabello et al 2005, Sawant et al 2009, Virdis et al 2010.…”

Section: Discussionmentioning

confidence: 63%

“…Virdis et al (2010) reported high sensitivity to quinolones, but not to aminoglycoside in subclinical mastitis-causing staphylococci.…”

Section: Discussionmentioning

confidence: 99%

“…The emergence of multidrug resistance (MDR) in Staphylococcus spp. is a problem for concern in animal production and issues related to public health (Kumar et al 2009, Sawant et al 2009, Virdis et al 2010.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Antimicrobial resistance of Staphylococcus spp. from small ruminant mastitis in Brazil

et al. 2012

View full text Add to dashboard Cite

Pesq. Vet. Bras. 32(8) The study aimed to determine the antimicrobial resistance patterns and to identify molecular resistance markers in Staphylococcus spp. (n=210) isolated from small ruminant mastitis in Brazil. The antimicrobial resistance patterns were evaluated by the disk diffusion test and by detection of the presence of mecA, blaZ, ermA, ermB, ermC and msrA genes by PCR. The efϐlux pump test was performed using ethidium bromide and bioϐilm production was determined by Congo red agar test along with PCR for detection of the icaD gene. The isolates were most resistant to amoxicillin (50.0%), streptomycin (42.8%), tetracycline (40.4%), lincomycin (39.0%) and erythromycin (33.8%). Pan-susceptibility to all tested drugs was observed in 71 (33.8%) isolates and 41 Staphylococcus isolates were positive for the efϐlux pump. Although phenotypic resistance to oxacillin was observed in 12.8% of the isolates, none harbored the mecA gene. However, 45.7% of the isolates harbored blaZ indicating that beta-lactamase production was the main mechanism associated with staphylococci resistance to beta-lactams in the present study. The other determinants of resistance to antimicrobial agents ermA, ermB, ermC, and msrA were observed in 1.4%, 10.4%, 16.2%, and 0.9% of the isolates, respectively. In addition, the icaD gen was detected in 32.9% of the isolates. Seventy three isolates (54 from goats and 19 from sheep) were negative for all resistance genes tested and 69 isolates presented two or more resistance genes. Association among blaZ, ermA, ermB, ermC and efϐlux pump were observed in 17 isolates, 14 of which originated from goats and three from sheep. The data obtained in this study show the resistance of the isolates to beta-lactamics, which may be associated with the use of antimicrobial drugs without veterinary control.

show abstract

Section: Discussioncontrasting

confidence: 47%

Section: Discussionmentioning

confidence: 63%

“…Virdis et al (2010) reported high sensitivity to quinolones, but not to aminoglycoside in subclinical mastitis-causing staphylococci.…”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Antimicrobial resistance of Staphylococcus spp. from small ruminant mastitis in Brazil

et al. 2012

View full text Add to dashboard Cite

show abstract

“…The prevalence of subclinical mastitis in small ruminants averages 6.5 and 40.2% (6)(7)(8)(9)(10)(11)(12)(13). The (14)(15)(16)(17).…”

Section: Introductionmentioning

confidence: 99%

The Prevalance, Etiology and Antimicrobial Susceptibility of the Microorganisms in Subclinical Mastitis in Goats

Doğruer

Saribay

Aslantaş

et al. 2016

Atatürk Üniversitesi Vet. Bil. Derg.

View full text Add to dashboard Cite

Abstract:This study was performed to determine the prevalance, causative microorganisms and antimicrobial susceptibility of microorganisms in goat subclinical mastitis around Hatay. A total of 1010 mammary halves of 505 goats were examined by California Mastitis Test (CMT). The somatic cell counts (SCC) were determined by microscopic method. Isolation and identification of microorganisms were carried out by conventional microbiologic methods. Staphylococci were further differentiated by API-STAPH system. Antimicrobial susceptibility was determined by disc diffusion method. The prevalance of the subclinical mastitis was found 8.71 %. The most prevalent microorganism was staphylococci (71.5%). Microorganisms except Staphylococci were Streptococci (8%), Bacillus spp. (5.7%), Escherischia coli (4.5%), Corynebacterium spp. (3.4%), Pseudomonas spp. (2.3%) and Acinetobacteri spp. (2.3%). In addition, mix infection was defined in 2.3% of samples. Highly resistance was found against penicilin, erythromycin, oxytetracycline, gentamicin, amoxicillin. Slightly resistance was found against enrofloxacin, amoxicillin-clavulonic acid, kanamycin plus cephalexin and there was no resistance against cefalotin. It was concluded that, prevalance of subclinical mastitis should be cared; also staphylococci especially Coagulase negative staphylococci are the most commonly isolated bacteria in subclinical mastitis around Hatay. In goat mastitis diagnosis strong positive CMT results should be cared. CMT and SCC results should be supported with microbiologic tests. 3) de izole edildi. Ek olarak örneklerin % 2.3'ünde miks enfeksiyon saptandı. Antibiyotik duyarlılık testlerine göre penisilin, eritromisin, oksitetrasiklin, gentamisin, amoksisilin'e karşı yüksek oranda direç gözlendi. Enrofloksasin, amoksisilin klavulonik asit, kanamisin ve sefaleksin'e karşı orta düzeyde direnç saptanırken, sefalotine karşı direnç yoktu. Sonuç olarak Hatay çevresinde keçi sürülerinde subklinik mastitisin prevalansının dikkate değer seviyelerde olduğu, koagülaz negatif stafilokokların en fazla izole edilen mikroorganizmalar olduğu belirlendi. Keçilerde subklinik mastitis tanısında kuvvetli CMT reasksiyonlarının dikkate alınması gerektiği, CMT ve somatik hücre sayılarının mikrobiyolojik yöntemlerle desteklenmeleri gerektiği kanısına varıldı.

show abstract

Rapid Phenotypic Antibiotics Susceptibility Analysis by a 3D Printed Prototype

Riester,

Kaiser,

Laufer

et al. 2024

Advanced Science

View full text Add to dashboard Cite

One of the most important public health concerns is the increase in antibiotic‐resistant pathogens and corresponding treatment of associated infections. Addressing this challenge requires more efficient use of antibiotics, achievable by the use of evidence‐based, effective antibiotics identified by antibiotic susceptibility testing (AST). However, the current standard method of phenotypic AST used for this purpose requires 48 h or more from sample collection to result. Until results are available, broad‐spectrum antibiotics are used to avoid delaying treatment. The turnaround time must therefore be shortened in order for the results to be available before the second administration of antibiotics. The phenotypic electrochemical AST method presented here identifies effective antibiotics within 5–10 h after sampling. Spiked serum samples, including polymicrobial samples, with clinically relevant pathogens and respective concentrations commonly found in bloodstream infections (Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae, and Pseudomonas aeruginosa) are used. Direct loading of the test with diluted serum eliminates the need for a pre‐culture, as required by existing methods. Furthermore, by combining several electrochemical measurement procedures with computational analysis, allowing the method to be used both online and offline, the AST achieves a sensitivity of 94.44% and a specificity of 95.83% considering each replicate individually.

show abstract

Antibiotic Resistance inStaphylococcus aureusand Coagulase Negative Staphylococci Isolated from Goats with Subclinical Mastitis

Cited by 110 publications

References 20 publications

Antimicrobial resistance of Staphylococcus spp. from small ruminant mastitis in Brazil

Antimicrobial resistance of Staphylococcus spp. from small ruminant mastitis in Brazil

The Prevalance, Etiology and Antimicrobial Susceptibility of the Microorganisms in Subclinical Mastitis in Goats

Rapid Phenotypic Antibiotics Susceptibility Analysis by a 3D Printed Prototype

Contact Info

Product

Resources

About