Antibiotic Resistance in Proteus mirabilis: Mechanism, Status, and Public Health Significance

Alqurashi, Ebtehal H.; Elbanna, Khaled; Ahmad, Iqbal; Abulreesh, Hussein H.

doi:10.22207/jpam.16.3.59

Cited by 8 publications

(3 citation statements)

References 82 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Finally, the prediction of antibiotic resistance genes within the genomes of the clinical isolates of P. mirabilis provided interesting insights for this study. P. mirabilis is described as naturally resistant to polymyxins and tetracyclines and susceptible to β-lactams, chloramphenicol, fluoroquinolones, and aminoglycosides [ 4 , 72 , 73 ]. Besides that, over time, more and more P. mirabilis strains harboring other AMR genes were isolated, such as those containing bla TEM genes, which confer resistance to the first generation of beta-lactams (such penicillin), the mutated variant of gyrB , which confers resistance to fluoroquinolones, and the aacs and aphs genes, which confer resistance to aminoglycosides [ 4 , 72 , 73 ].…”

Section: Discussionmentioning

confidence: 99%

“…P. mirabilis is described as naturally resistant to polymyxins and tetracyclines and susceptible to β-lactams, chloramphenicol, fluoroquinolones, and aminoglycosides [4,72,73]. Besides that, over time, more and more P. mirabilis strains harboring other AMR genes were isolated, such as those containing bla TEM genes, which confer resistance to the first generation of beta-lactams (such penicillin), the mutated variant of gyrB, which confers resistance to fluoroquinolones, and the aacs and aphs genes, which confer resistance to aminoglycosides [4,72,73]. In fact, bla TEM genes were found in three out of the ten P. mirabilis strains evaluated in this study.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Isolation and Characterization of the Acadevirus Members BigMira and MidiMira Infecting a Highly Pathogenic Proteus mirabilis Strain

da Silva,

Bens,

Santos

et al. 2023

Microorganisms

View full text Add to dashboard Cite

Proteus mirabilis is an opportunistic pathogen and is responsible for more than 40% of all cases of catheter-associated urinary tract infections (CAUTIs). Healthcare-associated infections have been aggravated by the constant emergence of antibiotic-resistant bacterial strains. Because of this, the use of phages to combat bacterial infections gained renewed interest. In this study, we describe the biological and genomic features of two P. mirabilis phages, named BigMira and MidiMira. These phages belong to the Acadevirus genus (family Autographiviridae). BigMira and MidiMira are highly similar, differing only in four missense mutations in their phage tail fiber. These mutations are sufficient to impact the phages’ depolymerase activity. Subsequently, the comparative genomic analysis of ten clinical P. mirabilis strains revealed differences in their antibiotic resistance profiles and lipopolysaccharide locus, with the latter potentially explaining the host range data of the phages. The massive presence of antimicrobial resistance genes, especially in the phages’ isolation strain P. mirabilis MCS, highlights the challenges in treating infections caused by multidrug-resistant bacteria. The findings reinforce BigMira and MidiMira phages as candidates for phage therapy purposes.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Isolation and Characterization of the Acadevirus Members BigMira and MidiMira Infecting a Highly Pathogenic Proteus mirabilis Strain

da Silva,

Bens,

Santos

et al. 2023

Microorganisms

View full text Add to dashboard Cite

show abstract

“…Finally, the prediction of antibiotic resistance genes within the genomes of the clinical isolates of P. mirabilis provided interesting insights for this study. P. mirabilis is described as naturally resistant to polymyxins and tetracyclines and susceptible to β-lactams, chloramphenicol, fluoroquinolones, and aminoglycosides [4,73,74]. Besides that, over time, more and more P. mirabilis strains harboring other AMR genes were isolated, such as those containing blaTEM genes, which confer resistance to the first generation of beta-lactams (such penicillin), the mutated variant of gyrB, which confers resistance to fluoroquinolones, and the aacs and aphs genes, which confer resistance to aminoglycosides [4,73,74].…”

Section: Discussionmentioning

confidence: 99%

Isolation and characterization of lytic bacteriophages for the composition of a phage cocktail capable of reducing bacterial biofilms in oil-related environments

Silva

View full text Add to dashboard Cite

Phages are viruses that infect bacteria and since their discovery in the early 20th century, they have been used to control bacterial growth. This work was divided into three chapters. The first genomically and biologically characterizes the Enterobacter phage vB_EclM-UFV01, presenting general characteristics of its genus, the Karamviruses. Chapter 2, examines the capacity of a phage cocktail (composed by enterobacteria-isolated phages) to reduce the biofilm of sulfate-reducing bacteria. The formulation of the cocktail, named Petro01, was performed by selecting phages from the collection of the Laboratory of Molecular Immunovirology at UFV and ended up being composed of three phages of the Tequatrovirus genus (briefly characterized genomically in this chapter) and one of the Karamvirus genus (described in Chapter 1). The stability of Petro01 under conditions similar to those found in petroleum-related environments, its shelf life at high storage temperatures, the supernatant composition (LB enriched with conservative compounds), as well as the propagation capacity of the phages that make up the cocktail in bench and a 12 L bioreactor, was also evaluated. Petro01 showed significant biotechnological potential and positive capacity for the composition of a phage-based product. Chapter 3 moves away from the environmental application of bacteriophages and characterizes, biologically and genomically, the Proteus mirabilis phages BigMira UFV01 and MidiMira UFV02, providing an overview of the common features of their genus, the Acadeviruses. It also presents the genomic evaluation of 10 strains of P. mirabilis, isolated in clinical environments, focusing on the LPS locus and the antimicrobial resistance profile. All phages described in this study have good potential as antibacterial agents. Keywords: Bacteriophages. Industrial microbiology. Sulfate-reducing bacteria. Bioproducing.

show abstract

Development of bacterial resistance in Germany from 2008 to 2022 — major culprit pathogens, antibacterial drugs, and prescribing practices

Bindel,

Seifert

2024

Naunyn-Schmiedeberg's Arch Pharmacol

View full text Add to dashboard Cite

Rising bacterial resistance is a global threat, causing rising financial burdens on healthcare systems and endangering effective treatment of bacterial infections. To ensure the efficacy of antibacterial drugs, it is essential to identify the most dangerous pathogens and vulnerable antibacterial drugs. Previous research by our group suggested irrational outpatient prescribing practices in Germany, supporting a growing bacterial resistance. This study analyses developments and characteristics for the ten most prescribed antibacterial drugs in Germany from 2008 to 2022. Conclusions are based on the development of bacterial resistance levels and an analysis of correlations between pathogens. We identified cefuroxime axetil, sulfamethoxazole-trimethoprim and nitrofurantoin as the most problematic drugs. Particularly problematic pathogens include E. faecalis, E. faecium, K. pneumoniae, and P. mirabilis. Besides increasing bacterial resistance, they are characterised by a high proportion of significant positive correlations, indicating a high potential for mutually reinforcing resistance development. Alarmingly, most of the antibacterial drugs analysed showed a growing resistance to at least one of the analysed pathogens. In most cases, the best treatment option is threatened by increasing bacterial resistance. We also identified several differences between current bacterial resistance data and therapeutic guidelines. In aggregate, our findings support irrational prescribing behaviour and underscore the urgent need for improved prescribing practices to counter rising bacterial resistance in Germany. Moreover, therapeutic guidelines for bacterial infections, the “holy grail” of pharmacotherapy, must be updated more frequently.

show abstract

Antibiotic Resistance in Proteus mirabilis: Mechanism, Status, and Public Health Significance

Cited by 8 publications

References 82 publications

Isolation and Characterization of the Acadevirus Members BigMira and MidiMira Infecting a Highly Pathogenic Proteus mirabilis Strain

Isolation and Characterization of the Acadevirus Members BigMira and MidiMira Infecting a Highly Pathogenic Proteus mirabilis Strain

Isolation and characterization of lytic bacteriophages for the composition of a phage cocktail capable of reducing bacterial biofilms in oil-related environments

Development of bacterial resistance in Germany from 2008 to 2022 — major culprit pathogens, antibacterial drugs, and prescribing practices

Contact Info

Product

Resources

About