Antibiotic Resistance in Staphylococci Adherent to Cortical Bone

Webb, Lawrence X.; Holman, Jeffrey; Araujo, Bill de; Zaccaro, Daniel; Gordon, E. S.

doi:10.1097/00005131-199402000-00007

Cited by 29 publications

(19 citation statements)

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“…Bacteria are known to colonize on metal implants and form adherent bio-films 26 . Additionally, osteomyelitis is found to develop preferentially beneath the surfaces of fixation devices adjacent to bone 26,27 .…”

Section: Discussionmentioning

confidence: 99%

Cefazolin embedded biodegradable polypeptide nanofilms promising for infection prevention: A preliminary study on cell responses

Ogle

Jiang

et al. 2010

Journal Orthopaedic Research

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Implant-associated infection is a serious complication in orthopedic surgery, and endowing implant surfaces with antibacterial properties could be one of the most promising approaches for preventing such infection. In this study, we developed cefazolin loaded biodegradable polypeptide multilayer nanofilms on orthopedic implants. We found that the amount of cefazolin released could be tuned. A high local concentration of cefazolin was achieved within the first a few hours and therefore may inhibit bacterial colonization in the critical post-implantation period. The developed cefazolin loaded nanofilms showed their in vitro efficacy against Staphylococcus aureus (S. aureus); the more antibiotics loaded, the longer the nanocoated implant had antibacterial properties. More interestingly, antibiotic-loaded polypeptide multilayer nanofilms also improved osteoblast bioactivity including cell viability and proliferation. These findings suggested that biodegradable polypeptide multilayer nanofilms as antibiotic carriers at the implant/tissue interface are compatible with human cells such as osteoblasts and bactericidal to bacteria such as S. aureus. These characteristics could be promising for preventing implant-associated infection and potentially improving bone healing.

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Section: Discussionmentioning

confidence: 99%

Cefazolin embedded biodegradable polypeptide nanofilms promising for infection prevention: A preliminary study on cell responses

Ogle

Jiang

et al. 2010

Journal Orthopaedic Research

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“…Finally, in nature, bacteria adhere to surfaces . MIC testing against bacteria adherent to human tissue surfaces or biomaterials has demonstrated increased bacterial resistance compared to MIC testing in the microbiology laboratory with bacteria in the planktonic phase (Gristina et al 1989, 1991, Naylor et al 1990, Webb et al 1994. This is partly explained by the creation of a biofilm that is impermeable to antibiotics.…”

Section: Discussionmentioning

confidence: 99%

High local concentrations without systemic adverse effects after impaction of netilmicin-impregnated bone

Witsø¹,

Persen²,

Benum³

et al. 2004

Acta Orthopaedica Scandinavica

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Background When cancellous bone is impregnated with antibiotics the subsequent release of antibiotics from the bone shows a high early release. Hence, impaction of large amounts of netilmicin-impregnated bone may cause toxic netilmicin values in serum.Patients and methods We studied kidney and otovestibular function after impacting 50 g of netilmicin-impregnated cancellous bone during revision hip or knee arthroplasty in 20 patients. The bone was impacted in the acetabulum (n = 8), proximal femur (n = 9) and distal femur/proximal tibia (n = 3). Serum creatinine concentration was measured and audiometry was performed before and after the operation. Netilmicin concentrations in serum, joint fluid, and in urine were recorded postoperatively at regular intervals. We analyzed pharmacokinetics in two study groups receiving bone impregnated with netilmicin (50 mL), at either 50 mg netilmicin/mL (group I) or 100 mg netilmicin/mL (group II).Results Neither netilmicin-induced renal toxicity, nor otovestibular toxicity was registered. Peak serum netilmicin values in group I and group II were 0.9 (0.5-1.3) mg/L and 1.8 (0.6-4.0) mg/L, respectively (p = 0.04). Peak netilmicin concentrations in wound drainage fluid in group I and group II were 237 (9-647) mg/L and 561 (196-1132) mg/L, respectively (p = 0.01). In both groups, netilmicin was recovered in urine samples for approximately 4 weeks.Interpretation 50 grams of cancellous bone impregnated with 100 mg/mL netilmicin solution was impacted in the hip or knee joint with no adverse effects. Extremely high local concentrations of netilmicin in

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“…The nonadherent state of CS pellets versus bone substitutes, like polymethylmethacrylate, could be advantageous for antibiotic delivery in chronic osteomyelitis with antibiotic resistance (5). The potential role of local administration of antibiotics from CS pellets in healing and bone repair is reported in a case series of patients with osteomyelitis (6).…”

Section: Use Of Tobramycinimpregnated Calcium Sulphate Pellets In Addmentioning

confidence: 99%

Use of Tobramycin-Impregnated Calcium Sulphate Pellets in Addition to Oral Antibiotics

et al. 2007

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Antibiotic Resistance in Staphylococci Adherent to Cortical Bone

Cited by 29 publications

References 0 publications

Cefazolin embedded biodegradable polypeptide nanofilms promising for infection prevention: A preliminary study on cell responses

Cefazolin embedded biodegradable polypeptide nanofilms promising for infection prevention: A preliminary study on cell responses

High local concentrations without systemic adverse effects after impaction of netilmicin-impregnated bone

Use of Tobramycin-Impregnated Calcium Sulphate Pellets in Addition to Oral Antibiotics

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