Antibiotic resistance in the microbiota of periodontitis patients: an update of current findings

Ng, Ethan; Tay, John Rong Hao; Boey, Sean Kuan; Laine, Marja L.; Ivanovski, Sašo; Seneviratne, Chaminda Jayampath

doi:10.1080/1040841x.2023.2197481

Cited by 16 publications

(10 citation statements)

References 75 publications

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“…The low-to-negligible levels of P. gingivalis resistance to metronidazole and metronidazole plus amoxicillin in United States patients is consistent with global data [5,6,73], except for a high P. gingivalis metronidazole resistance rate (≥21.6%) detected in Columbia [71,72]. Only 1 study patient yielded P. gingivalis resistant in vitro to metronidazole, and none had P. gingivalis resistant to both metronidazole and amoxicillin, out of the 2193 P. gingivalis-positive periodontitis patients studied.…”

Section: Discussionsupporting

confidence: 78%

“…gingivalis in vitro resistance to amoxicillin increased to 2.8% of isolates in the United States over the 20 years under study. This compares to a much higher resistance rate (25%) to amoxicillin among P. gingivalis clinical isolates in Columbia [71] and slightly exceeds the widespread absence of P. gingivalis resistance to amoxicillin elsewhere in the world [5,6,73].…”

Section: Discussionmentioning

confidence: 80%

“…Importantly, the prevalence of antibiotic-resistant P. gingivalis and other periodontal pathogens varies considerably between countries and geographic regions [5,6], which underscores the need for region-specific surveillance monitoring of antibiotic resistance trends among periodontopathic bacterial species. Pioneering studies by van Winkelhoff et al [65,66] found differences in antibiotic resistance patterns of periodontal pathogens between the Netherlands and Spain to be correlated with differences in antibiotic exposure between the two countries.…”

Section: Discussionmentioning

confidence: 99%

“…The increase in clindamycin-resistant P. gingivalis to a 9.3% prevalence level in the United States is alarming and exceeded worldwide only by a 23.5% frequency of clindamycinresistant P. gingivalis strains in Colombia in South America, where there is greater population exposure to antibiotic-containing over-the-counter products than in the United States [71,72]. Meanwhile, clindamycin resistance in P. gingivalis is reported to be absent or low in the Netherlands, Germany, Switzerland, Japan, Spain, Italy, Turkey, Iran, and Brazil [5,6,73]. Consistent with our findings in the United States, anaerobic bacteria residing in non-oral body sites have also recently exhibited large increases in clindamycin resistance linked to the spread of rRNA-methylase-encoding erm antibiotic resistance genes [74].…”

Section: Discussionmentioning

confidence: 99%

“…Surveillance surveys to monitor the extent of antibiotic resistance in pathogenic bacteria are considered essential for evaluating the efficacy of antibiotic stewardship programs [3,4]. However, data on antimicrobial resistance among periodontal bacterial pathogens in the human oral cavity are presently limited and largely cover only single time points [5,6].…”

Section: Introductionmentioning

confidence: 99%

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Emergence of Antibiotic-Resistant Porphyromonas gingivalis in United States Periodontitis Patients

Rams,

Sautter,

van Winkelhoff

2023

Antibiotics

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Antibiotic resistance patterns of the major human periodontal pathogen Porphyromonas gingivalis were assessed over a 20-year period in the United States. Subgingival P. gingivalis was cultured pre-treatment from 2193 severe periodontitis patients during three time periods: 1999–2000 (936 patients), 2009–2010 (685 patients), and 2019–2020 (572 patients). The clinical isolates were tested for in vitro resistance to 4 mg/L for clindamycin and doxycycline, 8 mg/L for amoxicillin, and 16 mg/L for metronidazole, with a post hoc combination of data for metronidazole plus amoxicillin. Clindamycin-resistant P. gingivalis was significantly more prevalent in 2009–2010 (9.1% of patients) and 2019–2020 (9.3%; 15-fold increase) as compared to 1999–2000 (0.6%). P. gingivalis resistance to amoxicillin also significantly increased from 0.1% of patients in 1999–2000 to 1.3% in 2009–2010 and 2.8% (28-fold increase) in 2019–2020. P. gingivalis resistance to metronidazole, metronidazole plus amoxicillin, and doxycycline was low (≤0.5% prevalence), and statistically unchanged, over the 20-year period. These findings are the first to reveal marked increases over 20 years in clindamycin-resistant and amoxicillin-resistant P. gingivalis in United States periodontitis patients. Increased antibiotic resistance of P. gingivalis and other periodontitis-associated bacteria threatens the efficacy of periodontal antimicrobial chemotherapy.

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Section: Discussionsupporting

confidence: 78%

Section: Discussionmentioning

confidence: 80%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Emergence of Antibiotic-Resistant Porphyromonas gingivalis in United States Periodontitis Patients

Rams,

Sautter,

van Winkelhoff

2023

Antibiotics

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The effects of berteroin on inflammatory mediators and antioxidant enzymes expression in human periodontal ligament cells

Hosokawa,

Shimoyama

et al. 2023

Naunyn-Schmiedeberg's Arch Pharmacol

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Berteroin is a bioactive substance classi ed as an isothiocyanate found in cruciferous vegetables such as cabbage, arugula, and salad leaves. In this study, we aimed to determine whether berteroin exerts antiin ammatory effects on human periodontal ligament cells (HPDLCs), a resident cells of periodontal tissue. Berteroin suppressed interleukin (IL)-1β or tumor necrosis factor (TNF)-α-induced chemokines (C-C motif chemokine ligand (CCL)2, CCL20, C-X-C motif chemokine ligand (CXCL)10, IL-8, and IL-6) production and intercellular adhesion molecule (ICAM)-1 expression in HPDLCs. In addition, berteroin inhibited phosphorylation of IκB kinase (IKK)-α/β, nuclear factor (NF)-κB p65, and IκB-α and degradation of IκB-α in the NF-κB pathway induced by IL-1β or TNF-α stimulation. Moreover, berteroin could inhibit signal transducer and activator of transcription (STAT)3 phosphorylation in TNF-α-stimulated HPDLC. Furthermore, berteroin increased the expression of the antioxidant enzymes, heme oxygenase (HO)-1 and NAD(P)H quinone dehydrogenase (NQO)1, in IL-1β or TNF-α-stimulated HPDLCs. These results suggest that berteroin may decrease the production of in ammatory mediators in HPDLCs by suppressing the NF-κB pathway, and may also decrease the local reactive oxygen species (ROS) production in periodontal lesions by increasing the production of antioxidant enzymes.

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Cardamonin decreases inflammatory mediator expression in IL-1β-stimulated human periodontal ligament cells

Okamoto,

Hosokawa,

Hosokawa

et al. 2024

Mol Biol Rep

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Antibiotic resistance in the microbiota of periodontitis patients: an update of current findings

Cited by 16 publications

References 75 publications

Emergence of Antibiotic-Resistant Porphyromonas gingivalis in United States Periodontitis Patients

Emergence of Antibiotic-Resistant Porphyromonas gingivalis in United States Periodontitis Patients

The effects of berteroin on inflammatory mediators and antioxidant enzymes expression in human periodontal ligament cells

Cardamonin decreases inflammatory mediator expression in IL-1β-stimulated human periodontal ligament cells

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