Antibiotic resistance in triclosan tolerant fecal coliforms isolated from surface waters near wastewater treatment plant outflows (Morris County, NJ, USA)

Middleton, June H.; Salierno, James D.

doi:10.1016/j.ecoenv.2012.10.025

Cited by 66 publications

(32 citation statements)

References 52 publications

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“…16 Triclosan-resistant bacteria exhibiting resistance to other antimicrobials were also found in a wastewater-impacted river. 17 The effect of triclosan on ARGs in anaerobic wastewater communities, where ARGs are abundant, [18][19][20] is poorly understood. Because ARGs are contaminants of concern 21 with negative public health consequences, 22 it is important to understand if triclosan can have an impact on the proliferation of ARGs in anaerobic wastewater communities where triclosan exposure can be high.…”

Section: Introductionmentioning

confidence: 99%

The Impacts of Triclosan on Anaerobic Community Structures, Function, and Antimicrobial Resistance

McNamara

LaPara

Novak

2014

Environ. Sci. Technol.

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Triclosan is a widespread antimicrobial agent that accumulates in anaerobic digesters used to treat the residual solids generated at municipal wastewater treatment plants; there is very little information, however, about how triclosan impacts microbial communities in anaerobic digesters. We investigated how triclosan impacts the community structure, function and antimicrobial resistance genes in lab-scale anaerobic digesters. Previously exposed (to triclosan) communities were amended with 5, 50, and 500 mg/kg of triclosan, corresponding to the median, 95th percentile, and 4-fold higher than maximum triclosan concentration that has been detected in U.S. biosolids. Triclosan amendment caused all of the Bacteria and Archaea communities to structurally diverge from that of the control cultures (based on ARISA). At the end of the experiment, all triclosan-amended Archaea communities had diverged from the control communities, regardless of the triclosan concentration added. In contrast, over time the Bacteria communities that were amended with lower concentrations of triclosan (5 mg/kg and 50 mg/kg) initially diverged and then reconverged with the control community structure. Methane production at 500 mg/kg was nearly half the methane production in control cultures. At 50 mg/kg, a large variability in methane production was observed, suggesting that 50 mg/kg may be a tipping point where function begins to fail in some communities. When previously unexposed communities were exposed to 500 mg triclosan/kg, function was maintained, but the abundance of a gene encoding for triclosan resistance (mexB) increased. This research suggests that triclosan could inhibit methane production in anaerobic digesters if concentrations were to increase and may also select for resistant Bacteria. In both cases, microbial community composition and exposure history alter the influence of triclosan.

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Section: Introductionmentioning

confidence: 99%

The Impacts of Triclosan on Anaerobic Community Structures, Function, and Antimicrobial Resistance

McNamara

LaPara

Novak

2014

Environ. Sci. Technol.

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“…Not surprisingly, water samples from communities that lack access to clean water contain high numbers of bacteria (7)(8)(9). While a high bacterial count in the water supply itself poses an increased health risk (10), the presence of antibiotic-resistant bacteria makes this risk even more serious.…”

mentioning

confidence: 99%

Detection of Antibiotic Resistance Genes in Source and Drinking Water Samples from a First Nations Community in Canada

Fernando

Tun

Poole

et al. 2016

Appl Environ Microbiol

114

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Access to safe drinking water is now recognized as a human right by the United Nations. In developed countries like Canada, access to clean water is generally not a matter of concern. However, one in every five First Nations reserves is under a drinking water advisory, often due to unacceptable microbiological quality. In this study, we analyzed source and potable water from a First Nations community for the presence of coliform bacteria as well as various antibiotic resistance genes. Samples, including those from drinking water sources, were found to be positive for various antibiotic resistance genes, namely, ampC, tet(A), mecA, ␤-lactamase genes (SHV-type, TEM-type, CTX-M-type, OXA-1, and CMY-2-type), and carbapenemase genes (KPC, IMP, VIM, NDM, GES, and OXA-48 genes). Not surprisingly, substantial numbers of total coliforms, including Escherichia coli, were recovered from these samples, and this result was also confirmed using Illumina sequencing of the 16S rRNA gene. These findings deserve further attention, as the presence of coliforms and antibiotic resistance genes potentially puts the health of the community members at risk. IMPORTANCEIn this study, we highlight the poor microbiological quality of drinking water in a First Nations community in Canada. We examined the coliform load as well as the presence of antibiotic resistance genes in these samples. This study examined the presence of antibiotic-resistant genes in drinking water samples from a First Nations Community in Canada. We believe that our findings are of considerable significance, since the issue of poor water quality in First Nations communities in Canada is often ignored, and our findings will help shed some light on this important issue.A ntibiotic resistance in bacteria has been recognized as one of the greatest threats to human health by the World Health Organization (1). Overuse and misuse of antibiotics contribute to the buildup of selective pressure aiding the proliferation of antibiotic-resistant bacteria (2, 3). While hospital environments are notorious for selecting for antibiotic-resistant bacteria, it is now becoming increasingly evident that overuse and misuse of antibiotics are also creating a selective pressure outside hospital settings. Studies over the last few years have shown the presence of antibiotics and of antibiotic-resistant bacteria in the broader environment, including water supplies and soil samples (4). This is indeed alarming as the high number of antibiotic-resistant bacteria in communities makes the treatment of community-acquired infections increasingly challenging (5, 6).Not surprisingly, water samples from communities that lack access to clean water contain high numbers of bacteria (7-9). While a high bacterial count in the water supply itself poses an increased health risk (10), the presence of antibiotic-resistant bacteria makes this risk even more serious. Lack of access to clean and safe water is a problem that is generally associated with developing countries; however, this is a reality as wel...

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“…Among the antimicrobial agents tested, the highest resistance rates in the penicillin group were found for ampicillin (up to 18%) and piperacillin (up to 12%); in the cephalosporin group for cefalothin (up to 35%) and cefuroxime-Axetil (up to 11%); in the group of quinolones for nalidixic acid (up to 15%); and for trimethoprime/sulfamethoxazole (up to 13%) and for tetracycline (57%) (Reinthaler et al, 2013). Similarly, developments of antibiotic-resistance bacteria (Middleton and Salierno, 2012;Shah et al, 2012) and risk to aquatic organisms have been correlated with pharmaceutical residues presence in wastewater from inadequate wastewater treatment systems. Samples from surface water sites near wastewater treatment plants in Australia had a significant increase of antibiotic resistant E. coli (Watkinson et al, 2007).…”

Section: Anmentioning

confidence: 99%

Antibiotics and antibiotic resistant bacteria in wastewater: Impact on environment, soil microbial activity and human health

Meena¹,

Dotaniya²,

Saha³

et al. 2015

Afr. J. Microbiol. Res.

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Antibiotic resistance in triclosan tolerant fecal coliforms isolated from surface waters near wastewater treatment plant outflows (Morris County, NJ, USA)

Cited by 66 publications

References 52 publications

The Impacts of Triclosan on Anaerobic Community Structures, Function, and Antimicrobial Resistance

The Impacts of Triclosan on Anaerobic Community Structures, Function, and Antimicrobial Resistance

Detection of Antibiotic Resistance Genes in Source and Drinking Water Samples from a First Nations Community in Canada

Antibiotics and antibiotic resistant bacteria in wastewater: Impact on environment, soil microbial activity and human health

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