Antibiotic resistance in wild and commercial non-enterococcal Lactic Acid Bacteria and Bifidobacteria strains of dairy origin: An update

Nunziata, Luca; Brasca, Milena; Morandi, Stefano; Silvetti, Tiziana

doi:10.1016/j.fm.2022.103999

Cited by 38 publications

(15 citation statements)

References 128 publications

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“…Therefore, it is essential to determine the drug resistance profile of potentially probiotic strains that may be used in food products (Zawistowska-Rojek and Tyski 2018;Stefańska et al 2021). All bacterial strains tested in the present study were susceptible to tetracycline, chloramphenicol, and ampicillin, which is consistent with studies conducted by others (Rozman et al 2020;Nunziata et al 2022). There are also reports on the strains resistant to these antibiotics, e.g., isolates from animals or fermented milk drinks, which were resistant to ampicillin (Dec et al 2018;Yang and Yu et al 2019).…”

Section: Lacticaseibacillussupporting

confidence: 88%

“…Lactobacillaceae species, is usually associated with the presence of the cat gene (Stefańska et al 2021). This gene is often located on plasmids, potentially allowing the transfer of the resistance gene from lactic acid bacteria to pathogenic bacteria (Nunziata et al 2022).…”

Section: Lacticaseibacillusmentioning

confidence: 99%

“…In turn, resistance to tetracycline, usually located in the tetK, tetW, or tetM genes, could also be passed on to other strains, since these genes are located on mobile genetic elements (Nunziata et al 2022). Most LABs are also sensitive to clindamycin (Nunziata et al 2022).…”

Section: Lacticaseibacillusmentioning

confidence: 99%

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New Potentially Probiotic Strains Isolated from Humans – Comparison of Properties with Strains from Probiotic Products and ATCC Collection

Zawistowska-Rojek

Kociszewska

Zaręba

et al. 2022

Polish Journal of Microbiology

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Lactic acid bacteria are used in various types of probiotic products. Due to the constantly growing probiotics market, new strains with pro-health properties are sought. The present study compared 39 strains of Lactobacillus, Lacticaseibacillus, and Lactiplantibacillus, isolated from probiotic products and healthy people. The current research aimed to search for new, potentially probiotic strains. For this purpose the relationship between Lactobacillaceae strains was carried out; moreover, the basic properties of probiotic microorganisms, such as survival at low pH and bile salt environment, antibiotic susceptibility, aggregation and antagonism were estimated. The properties of these isolates were also compared with the properties of probiotic strains from the ATCC collection. In comparing the genetic relationship (PFGE method) between the tested isolates, it was observed that some of them show a high degree of similarity. All tested strains tolerated an environment with a pH value of 3.0, and the addition of 0.3% bile salt; showed auto-aggregation properties and displayed antagonism against pathogenic microorganisms. In the present study, the bacteria were susceptible to tetracycline, chloramphenicol and ampicillin; the resistance to vancomycin depended on the bacteria type. All the properties were strain-depended. Most of the tested strains had properties comparable to the reference strains. Three L. acidophilus strains isolated from cervical swabs seem to be promising candidates for probiotic strains.

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Section: Lacticaseibacillussupporting

confidence: 88%

Section: Lacticaseibacillusmentioning

confidence: 99%

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New Potentially Probiotic Strains Isolated from Humans – Comparison of Properties with Strains from Probiotic Products and ATCC Collection

Zawistowska-Rojek

Kociszewska

Zaręba

et al. 2022

Polish Journal of Microbiology

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“…Antibiotic susceptibility test Probiotics should not contain transferable antibiotic resistance genes that can spread to other microorganisms or pathogens in both food and the GIS environment. 60 Table 6 presents the susceptibility of Bifidobacterium spp. to the different antibiotics tested.…”

Section: Safety Evaluationmentioning

confidence: 99%

Isolation, identification, and in vitro probiotic characterization of forty novel Bifidobacterium strains from neonatal feces in Erzurum province, Türkiye

Güler,

Çetin,

Albayrak

et al. 2024

J Sci Food Agric

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BACKGROUNDNeonatal feces are one of the most important sources for probiotic isolation. The purpose of this study was the isolation and identification of Bifidobacterium spp. from neonatal feces and the evaluation of in vitro probiotic properties of strains including safety tests.RESULTSA total of 40 isolates were obtained from 14 healthy newborns' feces in Erzurum province, Türkiye. By their rep‐PCR patterns and 16S rRNA gene sequences, isolates were identified as 26 Bifidobacterium breve and 14 Bifidobacterium longum. Fifteen of the isolates tolerated bile salts and showed high resistance to simulated gastric juice. Isolates exhibited varying rates of auto‐aggregation and hydrophobicity. In addition, most of the isolates displayed antibacterial activity against Escherichia coli O157:H7, Staphylococcus aureus ATCC 29213, Salmonella Typhimurium RSHMB 95091, and Pseudomonas aeruginosa ATCC 9027. However, only one strain showed bile salt hydrolase activity and two strains showed the ability to produce H2O2. Bifidobacterium strains were generally sensitive to the tested antibiotics and lacked kanamycin, gentamicin, and streptomycin resistance genes, and hemolytic and DNAse activities. On the other hand, it was determined that five strains had various virulence genes including gelE, esp, efaAfs, hyl, and ace.CONCLUSIONResults of the present study suggested that B. longum BH28, B. breve BH4 and B. breve BH5 strains have the potential as probiotic candidates for further studies. © 2024 Society of Chemical Industry.

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“…In this study, Gram-positive and Gram-negative bacteria and fungi from the intestinal microbiome of UM-A volunteers (individuals whose gut microbiota is able to metabolize EA to Uro-A are defined as UM-A volunteers) were inhibited in vitro experiments by vancomycin, polymyxin B sulfate, and amphotericin B, respectively (Kohanski et al 2010 ; Nunziata et al 2022 ), and different intestinal microbiomes were obtained for the EA-converting in vitro fermentation assay. Liquid chromatography-mass spectrometry (LC–MS/MS) was used to analyze the transformation products of EA metabolized in vitro by the intestinal bacterial population of UM-A volunteers.…”

Section: Introductionmentioning

confidence: 99%

In vitro conversion of ellagic acid to urolithin A by different gut microbiota of urolithin metabotype A

He,

Bian,

Zhao

et al. 2024

Appl Microbiol Biotechnol

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The metabolite urolithin A, a metabolite of the dietary polyphenol ellagic acid (EA), has significant health benefits for humans. However, studies on the gut microbiota involved in ellagic acid metabolism are limited. In this study, we conducted in vitro fermentation of EA using human intestinal microbiome combined with antibiotics (vancomycin, polymyxin B sulfate, and amphotericin B). Liquid chromatography-mass spectrometry (LC–MS/MS) analysis demonstrated that the production capacity of urolithin A by gut microbiota co-treated with polymyxin B sulfate and amphotericin B (22.39 µM) was similar to that of untreated gut microbiota (24.26 µM). Macrogenomics (high-throughput sequencing) was used to analyze the composition and structure of the gut microbiota. The results showed that the abundance of Bifidobacterium longum, Bifidobacterium adolescentis, and Bifidobacterium bifidum in the gut microbiota without antibiotic treatment or co-treated with polymyxin B sulfate and amphotericin B during EA fermentation was higher than that in other antibiotic treatment gut microbiota. Therefore, B. longum, B. adolescentis, and B. bifidum may be new genera involved in the conversion of EA to urolithin A. In conclusion, the study revealed unique interactions between polyphenols and gut microbiota, deepening our understanding of the relationship between phenolic compounds like EA and the gut microbiota. These findings may contribute to the development of gut bacteria as potential probiotics for further development. Key points • Intestinal microbiome involved in ellagic acid metabolism. • Gram-positive bacteria in the intestinal microbiome are crucial for ellagic acid metabolism. • Bifidobacterium longum, Bifidobacterium adolescentis, and Bifidobacterium bifidum participate in ellagic acid metabolism.

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Antibiotic resistance in wild and commercial non-enterococcal Lactic Acid Bacteria and Bifidobacteria strains of dairy origin: An update

Cited by 38 publications

References 128 publications

New Potentially Probiotic Strains Isolated from Humans – Comparison of Properties with Strains from Probiotic Products and ATCC Collection

New Potentially Probiotic Strains Isolated from Humans – Comparison of Properties with Strains from Probiotic Products and ATCC Collection

Isolation, identification, and in vitro probiotic characterization of forty novel Bifidobacterium strains from neonatal feces in Erzurum province, Türkiye

In vitro conversion of ellagic acid to urolithin A by different gut microbiota of urolithin metabotype A

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