Antibiotic resistance monitoring in heterotrophic bacteria from anthropogenic-polluted seawater and the intestines of oyster Crassostrea hongkongensis

Wang, Rui Xuan; Wang, An‐Li; Wang, Jiang Yong

doi:10.1016/j.ecoenv.2014.07.028

Cited by 21 publications

(19 citation statements)

References 25 publications

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“…Different kinds of antibiotics are given to fish via food or directly added to the water to control bacterial fish diseases. Frequently, bacteria develop one or more resistance genes to the antibiotics used in aquaculture facilities and in surrounding aquatic environments (Wang, Wang, & Wang, ). Residues and surplus of used antibiotics accumulate in surrounding sediments.…”

Section: Discussionmentioning

confidence: 99%

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Determination and transferability of plasmid-mediated antibiotic resistance genes of the bacteria isolated from rainbow trout

et al. 2017

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Antibiotic resistance and the presence of resistance genes (ARGs) were investigated in the bacteria isolated from rainbow trout (Oncorhynchus mykiss) from different trout farms located in Turkey. The most frequent types of antibiotic resistance were towards β‐lactams (cephalothin [70% of bacterial isolates], amoxicillin [63%], ampicillin [62%], ticarcillin [56%], aztreonam [51%]), macrolide [erythromycin, 68%] and sulphonamide [sulphamethoxazole, 51%]. Of bacterial isolates, 51% were multiple drug resistant (MDR), while 35% of the isolates were extensively drug resistant (XDR). None of isolates were pandrug resistant (PDR). The most common ARGs were ampC (36%) and sul1 (24%). The class 1 integron gene cassette was detected in 51% of the bacteria. There was a strong positive correlation between the antibiotic resistance rate and the presence of ARGs (r2 = .932). Gene encodes blaCTX‐M1, one of the extended spectrum beta‐lactamase enzymes, was first described in Aeromonas caviae, Photobacterium damselae, Pseudomonas luteola and Burkholderia cepacia. It was determined that 35% of the bacteria harboured at least one plasmid. Plasmid‐mediated ARGs were identified to be tetracyclines (tetA, tetB, tetC, tetD), sulphonamides (sul1, sul3) and β lactams (ampC, blapse). Thus, results suggest that ARG contamination situation deliberates resistance to tetracycline, aminoglycoside, chloramphenicol and sulphonamide. Therefore, the presence and activity of ARGs in fish and in environmental bacteria may play an important role in the spread of resistance genes among bacteria by transposition or integron gene cassettes.

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Section: Discussionmentioning

confidence: 99%

“…Frequently, bacteria develop one or more resistance genes to the antibiotics used in aquaculture facilities and in surrounding aquatic environments (Wang, Wang, & Wang, 2014). Residues and surplus of used antibiotics accumulate in surrounding sediments.…”

Section: Discussionmentioning

confidence: 99%

Determination and transferability of plasmid-mediated antibiotic resistance genes of the bacteria isolated from rainbow trout

et al. 2017

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“… 3 Different studies confirmed that the antibiotic resistance in bacterial pathogens can be induced and spread rapidly among bacterial species. 4 , 5 …”

Section: Introductionmentioning

confidence: 99%

Novel vancomycin–peptide conjugate as potent antibacterial agent against vancomycin-resistant <em>Staphylococcus aureus</em>

Jelínková¹,

Šplíchal²,

Jiménez³

et al. 2018

IDR

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BackgroundIncrease in vancomycin (Van)-resistant bacterial strains including vancomycin-resistant Staphylococcus aureus (VRSA) and lack of new effective antibiotics have become a formidable health problem.Materials and methodsWe designed a new conjugate composed of Van and a peptide Hecate (Hec; Van/Hec), and its potential antimicrobial activity was evaluated.ResultsResults from disk diffusion test, time-kill assay, determination of minimum inhibitory concentration (MIC), microscopy, and comet assay showed strong antimicrobial effects of Van/Hec against wild-type, methicillin-resistant Staphylococcus aureus (MRSA) and VRSA. Microscopy revealed that the exposure to Van/Hec results in disruption of bacterial cell integrity in all tested strains, which was not observed in case of Van or Hec alone.ConclusionOverall, we showed that the preparation of conjugates from antibiotics and biologically active peptides could help us to overcome the limitation of the use of antibiotic in the treatment of infections caused by multidrug-resistant bacteria.

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“…The pathogenic bacterium BV2 was found resistant to kanamycin and clindamycin while it was sensitive to all other antibiotics tested (Table 3), suggesting the possibility of employing chemotherapeutic agents to control this pathogen. However, selective pressure when using antibiotics may increase or even induce antibiotic resistance among bacteria (Alderman et al, 1998;Smith, 2008;Heuer et al, 2009;Wang et al, 2014;Wang et al, 2015) and hence use of antibiotics in aquaculture facilities should be controlled strictly. Other control methods such as the use of probiotics and prebiotics (Ravi et al, 2007), UV treatment of seawater, disinfection and frequent cleaning of facilities and also lowering stocking density to avoid stress to abalones can be tried preemptively before diseases occur.…”

Section: Sensitivity To Antibioticsmentioning

confidence: 99%

Isolation and characterisation of Vibrio harveyi as etiological agent of foot pustule disease in the abalone Haliotis discus hannai Ino 1953

Wang¹,

Yao²,

Liu³

et al. 2018

Indian J. Fish.

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Foot pustule disease is an important disease affecting abalones. In the present study, microbiological and histopathological investigations were carried out in the Japanese abalone Haliotis discus hannai, affected by foot pustule disease. Diseased abalones became lethargic, weak and eventually died. The remarkable symptoms were apparent foot pustules and atrophy of the foot muscle. A predominant bacterium designated as BV2 was isolated from the pustules of diseased abalones. Experimental infection by immersion challenge showed that BV2 was virulent to abalones and caused symptoms of foot pustule disease with median lethal dose value of ≈7.76×10 5 cfu ml -1 . BV2 was infectious to all tested abalones with high mortality. Histopathological investigations showed degeneration and collapse of foot muscles as well as connective tissues. Tissues in round pustules were necrotic and disorganised. The BV2 bacterium was identified as Vibrio harveyi based on the results of phenotypic and biochemical tests as well as 16S rRNA gene sequence analysis.. The bacterium was found resistant to kanamycin and clindamycin, and sensitive to other 11 antibiotics tested.

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Antibiotic resistance monitoring in heterotrophic bacteria from anthropogenic-polluted seawater and the intestines of oyster Crassostrea hongkongensis

Cited by 21 publications

References 25 publications

Determination and transferability of plasmid-mediated antibiotic resistance genes of the bacteria isolated from rainbow trout

Determination and transferability of plasmid-mediated antibiotic resistance genes of the bacteria isolated from rainbow trout

Novel vancomycin–peptide conjugate as potent antibacterial agent against vancomycin-resistant <em>Staphylococcus aureus</em>

Isolation and characterisation of Vibrio harveyi as etiological agent of foot pustule disease in the abalone Haliotis discus hannai Ino 1953

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Antibiotic resistance monitoring in heterotrophic bacteria from anthropogenic-polluted seawater and the intestines of oyster Crassostrea hongkongensis

Cited by 21 publications

References 25 publications

Determination and transferability of plasmid-mediated antibiotic resistance genes of the bacteria isolated from rainbow trout

Determination and transferability of plasmid-mediated antibiotic resistance genes of the bacteria isolated from rainbow trout

Novel vancomycin&ndash;peptide conjugate as potent antibacterial agent against vancomycin-resistant <em>Staphylococcus aureus</em>

Isolation and characterisation of Vibrio harveyi as etiological agent of foot pustule disease in the abalone Haliotis discus hannai Ino 1953

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Novel vancomycin–peptide conjugate as potent antibacterial agent against vancomycin-resistant <em>Staphylococcus aureus</em>