Antibiotic resistance of retail food and human <i>Campylobacter</i> isolates on the island of Ireland from 2001–2002

McGill, Kevina; Cowley, D.; Moran, L.; Scates, Pam; O'Leary, A.; Madden, Robert H.; Carroll, C.; McNamara, Eleanor; Moore, John E.; Fanning, Séamus; Collins, J. D.; Whyte, Paul

doi:10.1017/s0950268806006200

Cited by 18 publications

(12 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…On the other hand, some EU MSs reported a relatively high level of resistance to erythromycin, e.g., 4% and 18% in Belgium for C. jejuni and C. coli, respectively or 39% of the Campylobacter isolates in the Netherlands (Anonymous 2012b). Furthermore, a relatively low level of resistance of the strains tested to streptomycin (22.8%) obtained during our study is similar to other data where the percentage of such strains was higher among C. coli than C. jejuni (McGill et al 2006;Bardon et al 2011). In the present investigation none of the Campylobacter strains was resistant to another antimicrobial from the aminoglycoside group -gentamicin.…”

Section: Discussionsupporting

confidence: 91%

Prevalence, antimicrobial resistance, and molecular characterization of Campylobacter jejuni and C. coli isolated from retail raw meat in Poland

Wieczorek¹,

Szewczyk²,

Osek³

2012

Vet. Med.

View full text Add to dashboard Cite

ABSTRACT:The study was conducted to investigate the presence of Campylobacter spp. in meat sold to consumers at a retail market in Poland. Antimicrobial resistance and the presence of putative virulence genes of the isolates were also examined. A total of 558 meat samples, including beef (n = 105), pork (n = 85), and poultry (n = 368) were collected over an almost three year study period. It was found that 321 samples, all of them originating from poultry, were contaminated with Campylobacter spp. Most of the obtained isolates were classified as C. coli (189 strains, 58.9%), whereas C. jejuni was identified in 132 (41.1%) samples. All Campylobacter strains were susceptible to gentamicin and all but one C. coli isolate to erythromycin. On the other hand, the highest level of resistance among Campylobacter tested was to ciprofloxacin (91% for C. jejuni and 86.1% for C. coli) and nalidixic acid (89.3% for C. jejuni and 85% for C. coli). Furthermore, resistance to two or more classes of antibiotics was found in the majority (60.9%) of Campylobacter spp. and among them one C. coli strain showed resistance to four different classes of antimicrobials. Identification of virulence genes in the isolated Campylobacter showed that all of them had the flaA and cadF genes. The iam marker was found more often in C. coli strains (88.8%) compared to C. jejuni isolates (53.8%). On the other hand, the virB11 gene was identified only in 4.2% of C. coli and in 6.1% of C. jejuni strains, respectively. Furthermore, the prevalence of the cdtA, cdtB, and cdtC genes among C. jejuni strains was 97.7%, 93.2%, 96.2%, respectively, and was significantly higher than for C. coli regarding the cdtC (66.7%) gene. The obtained results showed that the presence of Campylobacter in retail meat may represent a threat to public health.

show abstract

Section: Discussionsupporting

confidence: 91%

Prevalence, antimicrobial resistance, and molecular characterization of Campylobacter jejuni and C. coli isolated from retail raw meat in Poland

Wieczorek¹,

Szewczyk²,

Osek³

2012

Vet. Med.

View full text Add to dashboard Cite

show abstract

“…This result agrees with the previous studies which suggested that none of C. jejuni isolates from humans and companion animals was resistant [16,28]. However, other studies reported 3-6.8% of erythromycin resistance in C. jejuni isolated from humans [7,18].…”

Section: Discussionsupporting

confidence: 92%

“…isolates from humans and companion animals [16,18,30]. A higher rate of resistance to tetracycline was also observed, mainly in C. coli isolated from pigs and broilers (62 and 52%, respectively) [30].…”

Section: Discussionmentioning

confidence: 98%

“…However, C. jejuni isolates from three different raising groups of dogs were not statistically significant. Since tetracycline has been used as an alternative treatment for Campylobacter gastroenteritis, the tetracycline resistance in the isolates of humans has been increasing [18,20], moreover, the usage of tetracyclines is increasing due to inexpensive cost and their broad spectrum of activity.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Characterization of antimicrobial resistance and application of RFLP for epidemiological monitoring of thermophilic Campylobacter spp. isolated from dogs and humans in Korea

Cho¹,

Kim²,

Min³

et al. 2014

Korean Journal of Veterinary Research

View full text Add to dashboard Cite

: An antimicrobial susceptibility test was conducted to compare the resistance rates among Campylobacter spp. isolates from dogs (n = 50) raised under diverse conditions and humans (n = 50). More than 60% of Campylobacter (C.) jejuni from dogs and humans showed resistance to nalidixic acid, enrofloxacin and ciprofloxacin. C. jejuni isolates from humans showed higher resistance to tetracycline (83.3%) and ampicillin (91.3%) than those from dogs. None of the C. jejuni or Campylobacter coli isolates from humans or dogs were resistant to erythromycin. Overall, 85% of Campylobacter spp. isolates showed a multidrug resistant phenotype. Nucleotide sequencing analysis of the gryA gene showed that 100% of NA R /CIP R C. jejuni isolates from dogs and humans had the Thr-86 t h -Ile mutation, which is associated with fluoroquinolone resistance. flaA PCR restriction fragment length polymorphism (RFLP) typing to differentiate the isolates below the species level revealed 12 different clusters out of 73 strains. The human isolates belonged to eight different RFLP clusters, while five clusters contained dog and human isolates.

show abstract

“…It should be pointed out that thermophilic Campylobacter spp. are intrinsically resistant to cephalosporins, including ceftiofur (6,27,42), and these compounds often are included in the selective medium for the isolation of Campylobacter (6). Regardless, we included ceftiofur in this panel, since it is frequently used in food animal production for the treatment of diseases caused by other bacterial pathogens.…”

Section: Mlst Of C Jejuni Isolatesmentioning

confidence: 99%

Emergence of a Tetracycline-ResistantCampylobacter jejuniClone Associated with Outbreaks of Ovine Abortion in the United States

Şahin¹,

Plummer²,

Jordan³

et al. 2008

J Clin Microbiol

122

179

View full text Add to dashboard Cite

Campylobacter infection is one of the major causes of ovine abortions worldwide. Historically, Campylobacter fetus subsp. fetus was the major cause of Campylobacter-associated abortion in sheep; however, Campylobacter jejuni is increasingly associated with sheep abortions. We examined the species distribution, genotypes, and antimicrobial susceptibilities of abortion-associated Campylobacter isolates obtained from multiple lambing seasons on different farms in Iowa, Idaho, South Dakota, and California. We found that C. jejuni has replaced C. fetus as the predominant Campylobacter species causing sheep abortion in the United States. Most strikingly, the vast majority (66 of 71) of the C. jejuni isolates associated with sheep abortion belong to a single genetic clone, as determined by pulsed-field gel electrophoresis, multilocus sequence typing, and cmp gene (encoding the major outer membrane protein) sequence typing. The in vitro antimicrobial susceptibilities of these isolates to the antibiotics that are routinely used in food animal production were determined using the agar dilution test. All of the 74 isolates were susceptible to tilmicosin, florfenicol, tulathromycin, and enrofloxacin, and 97% were sensitive to tylosin. However, all were resistant to tetracyclines, the only antibiotics currently approved in the United States for the treatment of Campylobacter abortion in sheep. This finding suggests that feeding tetracycline for the prevention of Campylobacter abortions is ineffective and that other antibiotics should be used for the treatment of sheep abortions in the United States. Together, these results indicate that a single tetracycline-resistant C. jejuni clone has emerged as the major cause of Campylobacter-associated sheep abortion in the United States.

show abstract

Antibiotic resistance of retail food and human Campylobacter isolates on the island of Ireland from 2001–2002

Cited by 18 publications

References 35 publications

Prevalence, antimicrobial resistance, and molecular characterization of Campylobacter jejuni and C. coli isolated from retail raw meat in Poland

Prevalence, antimicrobial resistance, and molecular characterization of Campylobacter jejuni and C. coli isolated from retail raw meat in Poland

Characterization of antimicrobial resistance and application of RFLP for epidemiological monitoring of thermophilic Campylobacter spp. isolated from dogs and humans in Korea

Emergence of a Tetracycline-ResistantCampylobacter jejuniClone Associated with Outbreaks of Ovine Abortion in the United States

Contact Info

Product

Resources

About