Antibiotic Resistance Pattern and Biofilm Formation Ability of Clinically Isolates of Salmonella enterica Serotype typhimurium

Abstract

Background:The emergence of antimicrobial-resistant bacteria with biofilm formation ability may be a major threat to public health and food safety and sanitation. Objectives: The aim of this study was to determine antibiotic resistance patterns and biofilm production characteristics of Salmonella typhimurium isolated from different species of birds. Materials and Methods:The antibiotic resistance patterns of 38 pre-identified isolates were screened by standard Kirby-Bauer discdiffusion method performed on Mueller-Hinton agar to a panel of 17 antibiotics. The extent of biofilm formation was measured by Microtiter plate (MTP)-based systems. Results: The highest antimicrobial resistance was detected against nalidixic acid (97%), followed by doxycycline (86%), colistin (84%), streptomycin (84%) and tetracycline (84%). All isolates were sensitive to amikacin (100%) and 97% and 95% of the isolates were sensitive to ceftazidime and ceftriaxone, respectively. Twenty one different antibiotic resistance patterns were observed among S. typhimurium isolates. According to the results of the microtitre plate biofilm assay, there was a wide variation in biofilm forming ability among S. typhimurium isolates. Most of the isolates (60.52%) were not capable of producing biofilm, while 26.31%, 7.89%, and 5.26% isolates were weak, strong and moderate biofilm producers, respectively. Conclusions: It was concluded that nearly all S. typhimurium isolates revealed a high multiple antibiotic resistant with low biofilm forming capabilities which proposed low association between biofilm formation and antibiotic resistance of a major food important pathogen.

Antibiotic resistance and biofilm formation ability of Salmonella serotypes isolated from beef, mutton, and meat contact surfaces at retail

“…In the present study, biofilm formation abilities of Salmonella isolates, recovered from red meat (beef and mutton) and meat contact surfaces at retails, were studied. All isolates had the ability to form biofilms, most of them (75.86%) were moderate, and the rest were strong biofilm producers, which was more different from other reports that showed most of the tested isolates were weak biofilm producers (Díez‐García, Capita, & Alonso‐Calleja, 2012; Ghasemmahdi et al., 2015; Nair et al., 2015). Chuah et al.…”

Detection of Biofilm Formation and Antibiotic Resistance of Salmonella in Broiler Chicken

“…Also both positive and negative biofilm formation were showed same degree of mortality and morbidity, Signs of depression, diarrhea, Omphilitis in young chicks, perhepatitis, pericarditis, Oophritis, redness in brain. The obtained results were agree with (Wang et al, 2013) who reported that no significant correlation between antimicrobial resistance and biofilm production as well as agree with (Ghasemmahdi et al, 2015) who demonstrated that all Salmonella typhimurium isolates showed a high multiple antibiotic resistant with low biofilm formation capabilities which proposed low association between biofilm formation and antibiotic resistance of a major food important pathogen. As well as the results were agree with (Apellanis et al, 2017) who reported that no relationship was found between biofilm production and antimicrobial resistance in Salmonella enteritidis strains.…”

Molecular Detection, Virulence Genes, Biofilm Formation, and Antibiotic Resistance of Salmonella enterica Serotype enteritidis Isolated from Poultry and Clinical Samples

“…These results were inconsistent with those reported from Turkey (34); but in agreement with the current study findings, a study conducted in Brazil showed the resistance about 88.8% to nalidixic acid (35). Also, Ghasemmahdi et al showed the resistance (97%) to nalidixic acid (36).…”