Antibiotic Resistance, Phylogenetic Group, and Genotyping Investigation in Escherichia coli Strains of Gut Flora in Patients with Colorectal Cancer in Iranian Population

Mirzarazi, Mahsa; Bandehpour, Mojgan; Hashemi, Ali; Vahidi, Mahmoud; Taghavi, Afsoon; Bashiri, Soroor; Kazemi, Bahram

doi:10.4103/bbrj.bbrj_132_21

Cited by 2 publications

(3 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thus, stool samples of 50 (25 females and 25 males) healthy people were collected. It should be noted that only the E.coli strains were isolated from each sample [ 17 ].…”

Section: Resultsmentioning

confidence: 99%

“…Statistical analysis was conducted to compare the isolates of E.coli of people with colorectal cancer and the E.coli isolated from healthy people. Thus, similar to the CDC patients, stools were collected, cultured, and E.coli strains were isolated from 50 people without any symptoms or relevance to the disease [ 17 ].…”

Section: Methodsmentioning

confidence: 99%

“…The phylogenetic groups of all isolate E.coli bacteria were investigated with PCR performed for chuA (Outer membrane hemin receptor ChuA), YjaA (stress response protein) , and Tsp E4C2(Tail-specific protease)genes (Table 2 ) [ 35 , 51 , 52 ]. In addition, the EcoR62 strain of E.coli containing the chuA, yjaA, and TspE4C2 genes, as the positive control, was considered in this study [ 17 , 51 ].…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

The OmpA of commensal Escherichia coli of CRC patients affects apoptosis of the HCT116 colon cancer cell line

et al. 2022

Self Cite

View full text Add to dashboard Cite

Background Colorectal cancer ranks third globally among all types of cancers. Dysbiosis of the gut microbiota of people with CRC is one of the effective agents in the tumorigenesis and metastasis in this type of cancer. The population of Escherichia coli strains, a component of gut microbiota, is increased in the gut of people with CRC compared with healthy people. So, E.coli strains isolated from these patients may have a role in tumorigenesis. Because the most isolated strains belong to the B2 phylogenuetic group, there seems to be a linkage between the bacterium components and malignancy. Material and methods In this study, the proteomic comparison between isolated Ecoli from CRC patients and healthy people was assayed. The isolated spot was studied by Two-dimensional gel electrophoresis (2DE) and Liquid chromatography-mass spectrometry (LC–MS). The results showed that the expression of Outer membrane protein A (OmpA) protein increased in the commensal E.coli B2 phylogenetic group isolated from CRC patients. Additionally, we analyzed the effect of the OmpA protein on the expression of the four genes related to apoptosis in the HCT116 colon cancer cell line. Results This study identified that OmpA protein was overexpressed in the commensal E.coli B2 phylogenetic group isolated from CRC patients compared to the E.coli from the control group. This protein significantly decreased the expression of Bax and Bak, pro-apoptotic genes, as well as the expression of P53 in the HCT116 Cell Line, P < 0.0001. LC–MS and protein bioinformatics results confirmed that this protein is outer membrane protein A, which can bind to nucleic acid and some of the organelle proteins on the eukaryotic cell surface. Conclusions According to our invitro and insilico investigations, OmpA of gut E.coli strains that belong to the B2 phylogenetic group can affect the eukaryotic cell cycle.

show abstract

“…Thus, stool samples of 50 (25 females and 25 males) healthy people were collected. It should be noted that only the E.coli strains were isolated from each sample [ 17 ].…”

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

The OmpA of commensal Escherichia coli of CRC patients affects apoptosis of the HCT116 colon cancer cell line

et al. 2022

Self Cite

View full text Add to dashboard Cite

show abstract

Identification and Evaluation of Survival-associated Common Chemoresistant Genes in Cancer

Patel,

Singh,

Gandupalli

et al. 2024

Biomedical and Biotechnology Research Journal

View full text Add to dashboard Cite

Background: Chemoresistance is a major challenge in the effective treatment of cancer patients. This study aimed to identify common chemoresistance-associated genes that impact cancer survival. Methods: RNA-sequencing datasets for colorectal cancer (CRC) and ovarian cancer (OC) were analyzed using the gene expression omnibus (GEO) database and R Studio. Survival analysis was conducted using patient data from the cBioPortal database, where patients were stratified into high- and low-risk groups based on gene expression levels. Molecular dynamics simulations were performed to compare the binding energies of inhibitors targeting the identified genes. Results: Six common chemoresistance-associated genes were identified in both CRC and OC: cyclin-dependent kinase 2 (CDK2), inhibitor of differentiation (ID1), DUSP6, c-JUN, PLA2G2A, and SKP2. The expression of these genes was also evaluated in chemosensitive and chemoresistant oral cancer samples. Survival analysis revealed that high expression of CDK2 and ID1 significantly reduced the disease-free survival (DFS) of CRC and oral cancer patients with Grade II, III, or IV tumors. Conversely, low expression of ID1 was associated with reduced DFS in OC patients. Molecular simulations showed that the inhibitors dinaciclib (for CDK2) and vinblastine (for ID1) had higher binding energies compared to other inhibitors. Conclusion: The present study highlights the significance of CDK2 and ID1 as important mediators of chemoresistance and their potential as therapeutic targets to improve the survival of cancer patients exhibiting drug resistance.

show abstract

Antibiotic Resistance, Phylogenetic Group, and Genotyping Investigation in Escherichia coli Strains of Gut Flora in Patients with Colorectal Cancer in Iranian Population

Cited by 2 publications

References 25 publications

The OmpA of commensal Escherichia coli of CRC patients affects apoptosis of the HCT116 colon cancer cell line

The OmpA of commensal Escherichia coli of CRC patients affects apoptosis of the HCT116 colon cancer cell line

Identification and Evaluation of Survival-associated Common Chemoresistant Genes in Cancer

Contact Info

Product

Resources

About