Antibiotic resistance profiles of representative wetland bacteria and faecal indicators following ciprofloxacin exposure in lab-scale constructed mesocosms

Effect of gold nanoparticles and ciprofloxacin on microbial catabolism: a community‐based approach

“…The types of mesocosms used have been previously described by Weber et al . The 4 mesocosm wetlands MW1, MW2, MW3, and MW4 all contained microbial communities that had demonstrated antibiotic tolerance, but the 4 mesocosm wetlands MW5, MW6, MW7, and MW8 did not contain induced antibiotic‐resistant microbial communities . These 8 different mesocosm wetland microbial communities were exposed to 0 µg/mL, 0.5 µg/mL, 1.0 µg/mL, or 2.0 µg/mL ciprofloxacin in triplicate in BIOLOG ECO plates, and their catabolic profile and function directly quantified.…”

Ecotoxicological assessment of antibiotics: A call for improved consideration of microorganisms

“…The Biolog-CLPP approach scores growth and respiration of bacteria in Biolog™ plates that provide a variety of different carbon sources (Garland, 1997) and has been frequently used as an endpoint for long-term studies of antibiotic effects in bacterial communities (Helt et al, 2012;Kong et al, 2006;Liu et al, 2014;Maul et al, 2006;Schmitt et al, 2004). Its main drawback is that only bacteria actively growing in the Biolog plates contribute to the result, thereby compromising the ecological relevance of the approach (Ros et al, 2008;Smalla et al, 1998).…”

Effects of solution and solid forms of 2-hydroxypropyl-3-piperazinyl-quinoline carboxylic acid methacrylate on antibacterial, physical and mechanical properties of polypropylene sheeting

“…The number of CFU of bacteria cells suspended in nutrient media were counted after contacting with the test specimen on nutrient agar, using ten-fold serial dilution method. 33,34 The test procedure followed ASTM E2149 (2001) for the determination of antimicrobial activity of an antimicrobial agent under dynamic contact conditions, as suggested by our previous studies. 9,19,25 The procedure began by preparing a bacterial cell suspension of E. coli (as the initial bacteria) at 10 8 CFU/cell in peptone solution media (1 g/L, pH 6.8-7.2).…”