Antibiotic-resistant Escherichia coli in deer and nearby water sources at Safari parks in Bangladesh

Sarker, Samun; Ahad, Abdul; Ghosh, Suparna; Mannan, Shahriar; Sen, Arup; Islam, Shariful; Bayzid,; Bupasha, Zamila Bueaza

doi:10.14202/vetworld.2019.1578-1583

Cited by 14 publications

(15 citation statements)

References 30 publications

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“…DNA from pure culture was extracted using conventional boiling method [ 47 ]. Briefly, each isolate cultured on nutrient agar medium and incubated overnight at 37 °C.…”

Section: Methodsmentioning

confidence: 99%

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Virulence and Antimicrobial Resistance Profiles of Salmonella enterica Serovars Isolated from Chicken at Wet Markets in Dhaka, Bangladesh

et al. 2021

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Virulent and multi drug resistant (MDR) Salmonellaenterica is a foremost cause of foodborne diseases and had serious public health concern globally. The present study was undertaken to identify the pathogenicity and antimicrobial resistance (AMR) profiles of Salmonellaenterica serovars recovered from chicken at wet markets in Dhaka, Bangladesh. A total of 870 cecal contents of broiler, sonali, and native chickens were collected from 29 wet markets. The overall prevalence of S. Typhimurium, S. Enteritidis, and untyped Salmonella spp., were found to be 3.67%, 0.57%, and 1.95% respectively. All isolates were screened by polymerase chain reaction (PCR) for eight virulence genes, namely invA, agfA, IpfA, hilA, sivH, sefA, sopE, and spvC. S. Enteritidis isolates carried all virulence genes whilst S. Typhimurium isolates carried six virulence genes except sefA and spvC. A diverse phenotypic and genotypic AMR pattern was found. Harmonic descending trends of resistance patterns were observed among the broiler, sonali, and native chickens. Interestingly, virulent and MDR Salmonella enterica serovars were found in native chicken, although antimicrobials were not used in their production cycle. The research findings anticipate that virulent and MDR Salmonella enterica are roaming in the wet markets which can easily anchor to the vendor, consumers, and in the food chain.

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“…DNA from pure culture was extracted using conventional boiling method [ 47 ]. Briefly, each isolate cultured on nutrient agar medium and incubated overnight at 37 °C.…”

Section: Methodsmentioning

confidence: 99%

“…DNA from pure culture was extracted using conventional boiling method [47]. Briefly, each isolate cultured on nutrient agar medium and incubated overnight at 37 • C. Few fresh colonies were harvested from overnight culture and suspended in nuclease free water.…”

Section: Dna Extractionmentioning

confidence: 99%

Virulence and Antimicrobial Resistance Profiles of Salmonella enterica Serovars Isolated from Chicken at Wet Markets in Dhaka, Bangladesh

et al. 2021

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“…Another drug resistance gene, AmpC, encodes AmpC β-lactamase that degrades penicillins [13]. aadB is responsible for the production of 2'-aminoglycoside nucleotidyltransferase enzyme that inactivates aminoglycoside antibiotics [14].…”

Section: Introductionmentioning

confidence: 99%

Advanced molecular characterization of enteropathogenic Escherichia coli isolated from diarrheic camel neonates in Egypt

Shahein¹,

Dapgh²,

Kamel³

et al. 2021

Vet World

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Background and Aim: Camels are important livestock in Egypt on cultural and economic bases, but studies of etiological agents of camelid diseases are limited. The enteropathogen Escherichia coli is a cause of broad spectrum gastrointestinal infections among humans and animals, especially in developing countries. Severe infections can lead to death. The current study aimed to identify pathogenic E. coli strains that cause diarrhea in camel calves and characterize their virulence and drug resistance at a molecular level. Materials and Methods: Seventy fecal samples were collected from diarrheic neonatal camel calves in Giza Governorate during 2018-2019. Samples were cultured on a selective medium for E. coli, and positive colonies were confirmed biochemically, serotyped, and tested for antibiotic susceptibility. E. coli isolates were further confirmed through detection of the housekeeping gene, yaiO, and examined for the presence of virulence genes; traT and fimH and for genes responsible for antibiotic resistance, ampC, aadB, and mphA. The isolates in the important isolated serotype, E. coli O26, were examined for toxigenic genes and sequenced. Results: The bacteriological and biochemical examination identified 12 E. coli isolates from 70 fecal samples (17.1%). Serotyping of these isolates showed four types: O26, four isolates, 33.3%; O103, O111, three isolates each, 25%; and O45, two isolates, 16.7%. The isolates showed resistance to vancomycin (75%) and ampicillin (66.6%), but were highly susceptible to ciprofloxacin, norfloxacin, and tetracycline (100%). The structural gene, yaiO (115 bp), was amplified from all 12 E. coli isolates and traT and fimH genes were amplified from 10 and 8 isolates, respectively. Antibiotic resistance genes, ampC, mphA, and aadB, were harbored in 9 (75%), 8 (66.6%), and 5 (41.7%), respectively. Seven isolates (58.3%) were MDR. Real-time-polymerase chain reaction of the O26 isolates identified one isolate harboring vt1, two with vt2, and one isolate with neither gene. Sequencing of the isolates revealed similarities to E. coli O157 strains. Conclusion: Camels and other livestock suffer various diseases, including diarrhea often caused by microbial pathogens. Enteropathogenic E. coli serotypes were isolated from diarrheic neonatal camel calves. These isolates exhibited virulence and multiple drug resistance genes.

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“…The collected rectal swab samples were enriched overnight in buffered peptone water (BPW) (Oxoid Ltd., Basingstoke, UK) [ 16 ]. A loopful of the enriched broth was inoculated onto MacConkey agar (Oxoid Ltd, Basingstoke, UK) and incubated for 24 h at 37°C.…”

Section: Methodsmentioning

confidence: 99%

Isolation of multidrug-resistant Escherichia coli, Staphylococcus spp., and Streptococcus spp. from dogs in Chattogram Metropolitan Area, Bangladesh

et al. 2020

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Antibiotic-resistant Escherichia coli in deer and nearby water sources at Safari parks in Bangladesh

Cited by 14 publications

References 30 publications

Virulence and Antimicrobial Resistance Profiles of Salmonella enterica Serovars Isolated from Chicken at Wet Markets in Dhaka, Bangladesh

Virulence and Antimicrobial Resistance Profiles of Salmonella enterica Serovars Isolated from Chicken at Wet Markets in Dhaka, Bangladesh

Advanced molecular characterization of enteropathogenic Escherichia coli isolated from diarrheic camel neonates in Egypt

Isolation of multidrug-resistant Escherichia coli, Staphylococcus spp., and Streptococcus spp. from dogs in Chattogram Metropolitan Area, Bangladesh

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